LOCUS       sequence-458135-       11590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 11590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NPHS1-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..11590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        3105..3484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3485..3688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3639..3659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3685..3709
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3919..3937
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3919..3936
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     3950..3969
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7195..7230
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             7240..7263
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8923..8967
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8968..8994
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(9134..9151)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9140..9251
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9369..9389)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9374..9703
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9554..9689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9616..9635
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9751..9798
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9817..10416
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9817..9836)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10313..10333
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10546..10667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10583..10602)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10637..10656
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(10720..11511,11512..11580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11343..11362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11581..11590,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 gctgagagat cccctcataa tttccccaaa gcgtaaccat gtgtgaataa attttgagct
     1681 agtagggttg cagccacgag taagtcttcc cttgttattg tgtagccaga atgccgcaaa
     1741 acttccatgc ctaagcgaac tgttgagagt acgtttcgat ttctgactgt gttagcctgg
     1801 aagtgcttgt cccaaccttg tttctgagca tgaacgcccg caagccaaca tgttagttga
     1861 agcatcaggg cgattagcag catgatatca aaacgctctg agctgctcgt tcggctatgg
     1921 cgtaggccta gtccgtaggc aggacttttc aagtctcgga aggtttcttc aatctgcatt
     1981 cgcttcgaat agatattaac aagttgtttg ggtgttcgaa tttcaacagg taagttagtt
     2041 gctagaaccc atggctcctt tgccgacgct gagtagattt taggtgacgg gtggtgacaa
     2101 tgagtccgtg tcgagcgctg attttttcgg cctttagagc gagatttata caatagaatt
     2161 tggcatgaga ttggattgct tttagtcagc ctcttatagc ctaaagtctt tgagtgacta
     2221 gatgacatat catgtaagtt gctgataggt ttccagtttt ccgctcctag gtctgcatat
     2281 tgtacttttc ctcttactcg acttaaccag taccaaccca gcttctcaac ggatttatac
     2341 catggcactt taaagccagc atcactgaca atgagcggtg tggtgttact cggtagaatg
     2401 ctcgcaaggt cggctagaaa ttggtcatga gctttctttg aacattgctc tgaaagcggg
     2461 aacgctttct cataaagagt aacagaacga ccgtgtagtg cgactgaagc tcgcaatacc
     2521 ataagtcgtt tttgctcacg aatatcagac cagtcaacaa gtacaatggg catcgtattg
     2581 cccgaacaga taaagctagc atgccaacgg tatacagcga gtcgctcttt gtggaggtga
     2641 cgattaccta acaatcggtc gattcgtttg atgttatgtt ttgttctcgc tttggttggc
     2701 aggttacggc caagttcggt aagagtgaga gttttacagt caagtaatgc gtggcaagcc
     2761 aacgttaagc tgttgagtcg ttttaagtgt aattcggggc agaattggta aagagagtcg
     2821 tgtaaaatat cgagttcgca catcttgttg tctgattatt gatttttcgc gaaaccattt
     2881 gatcatatga caagatgtgt atccacctta acttaatgat ttttaccaaa atcattaggg
     2941 gattcatcag tgcacagtgg cgttgactca atattggcca ttagccatat tattcattgg
     3001 ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa
     3061 tatgtacatt tatattggct catgtccaat atgaccgcca tgttggcatt gattattgac
     3121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     3181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     3661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     3721 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg
     3781 ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag
     3841 agtctatagg cccaccccct tggcttcgtt agaacgcggc tacaattaat acataacctt
     3901 atgtatcata cacatacgat ttaggtgaca ctatagaata acatccactt tgcctttctc
     3961 tccacaggtg tccactccca ggtccaactg cacctcggtt ctatcgaaaa cgcgcctgct
     4021 agcgccacca tgaagaccat cattgccctg agctacatct tctgcctggt gttcgctgcg
     4081 gccgcccaac ttgctattcc tgcttccgtg cctagaggct tttgggccct gcctgagaat
     4141 ctgacagtgg tggaaggcgc cagcgtggaa ctgagatgcg gagtgtctac acctggatct
     4201 gccgtgcagt gggccaaaga tggactgctg cttggccccg atcctagaat ccctggcttc
     4261 cccagataca gactggaagg cgatcctgcc agaggcgagt tccatctgca catcgaggcc
     4321 tgcgatctga gcgacgatgc cgagtatgag tgccaagtgg gcagatctga gatgggccct
     4381 gagctggtgt ctcccagagt gatcctgtct atcctggtgc ctcctaaact gctgctgctg
     4441 acacctgagg ccggcacaat ggttacatgg gtggccggac aagagtacgt ggtcaattgt
     4501 gtgtctggcg acgccaagcc tgctcctgat atcacaatcc tgctgagcgg ccagaccatc
     4561 agcgacatca gcgccaatgt gaatgagggc agccagcaga aactgttcac cgtggaagcc
     4621 accgccagag tgacacccag aagcagcgat aacagacagc tgctcgtgtg cgaagccagc
     4681 tctcctgctc tggaagcccc tatcaaggcc agcttcaccg tgaacgtgct gttccctcct
     4741 ggacctcctg tgattgagtg gcctggactg gatgagggac acgttcgagc tggacagagc
     4801 ctggaactgc cttgtgtggc tagaggcgga aatcctctgg ctaccctgca gtggctgaag
     4861 aatggccagc ctgtgtctac cgcctgggga acagaacata cacaggccgt ggccagatcc
     4921 gtgctggtca tgacagttag acctgaggat cacggcgccc agctgagctg tgaagcccat
     4981 aattctgtgt ccgccggcac acaagagcac ggcatcaccc tgcaagtgac ctttccacct
     5041 agcgccatca tcatcctggg cagcgcctct cagaccgaga acaagaatgt gaccctgtcc
     5101 tgcgtgtcca agagcagcag acctagagtg ctgctgagat ggtggcttgg atggcgtcag
     5161 ctgctgccca tggaagagac agtgatggat ggcctgcacg gcggccacat ctctatgagc
     5221 aatctgacct tcctggccag aagagaggac aacggcctga cactgacatg cgaggccttc
     5281 tccgaggcct ttaccaaaga gacattcaag aagtccctga tcctgaacgt gaagtacccc
     5341 gctcagaagc tgtggatcga aggccctcct gagggacaga aactgagagc tggaacaaga
     5401 gtgcgcctcg tgtgcctggc cattggagga aatcctgagc ctagcctgat gtggtacaag
     5461 gacagcagaa ccgtgaccga gagcagactg ccccaagagt ccagaagagt gcacctgggc
     5521 tctgtggaaa agagcggcag caccttcagc agagaactgg tgctggttac aggcccctcc
     5581 gacaaccagg ccaagtttac ctgtaaagcc ggccagctga gtgcctccac acagctggct
     5641 gttcagttcc cacctaccaa cgtgacaatc ctggccaacg cttctgctct gaggcctggg
     5701 gatgctctga atctgacttg cgtgtccgtg tccagcaatc ctccagtgaa tctgagctgg
     5761 gacaaagagg gcgagagact cgaaggtgtt gctgcccctc ctagaagggc cccttttaaa
     5821 ggatctgcag ccgctagaag cgtgctgctc caggtgtcat ccagagatca cggccagaga
     5881 gtgacctgta gagcccattc tgccgagctg agagaaaccg tgtctagctt ctaccggctc
     5941 aatgtgctgt acagacccga gttcctgggc gagcaggttc tggtggttac agctgttgaa
     6001 cagggcgaag ctctgctgcc cgtgtctgtg tctgctaatc ccgctcctga ggccttcaac
     6061 tggaccttca gaggctacag actgagccct gctggcggac ccagacacag gattctttct
     6121 agcggagccc tgcacctgtg gaacgtgacc agagccgatg atggcctgta tcagctgcac
     6181 tgccagaata gcgaaggcac agccgaagcc agactgagac tggatgtgca ctacgcccct
     6241 accatcagag cactgcagga ccccaccgaa gtgaatgtcg gcggatctgt ggatatcgtg
     6301 tgcaccgtgg acgctaaccc tatcctgcct ggcatgttca actgggagag actgggcgaa
     6361 gatgaggaag atcagtccct ggacgacatg gaaaagatca gcagaggccc taccggcagg
     6421 ctgagaattc atcatgccaa actggcccag gcaggcgcct accagtgtat cgttgataat
     6481 ggcgtggccc ctcctgctcg gagactgctg agactcgttg tcagatttgc ccctcaagtg
     6541 gaacacccca cacctctgac aaaagtggcc gctgccggcg atagcacatc ttctgctaca
     6601 ctgcattgca gagccagggg cgtgcccaac atcgtgttta cctggaccaa gaacggcgtg
     6661 cccctggacc tgcaagatcc tagatacacc gagcacacct accaccaagg cggagtgcat
     6721 agcagcctgc tgaccattgc taacgtgtca gccgctcagg actacgccct gtttacatgc
     6781 acagccacaa acgccctggg ctccgaccag acaaatatcc agctcgtgtc catcagcaga
     6841 cccgatcctc catctggcct gaaggtggtg tctctgacac ctcactctgt gggcctcgag
     6901 tggaagcctg gatttgatgg tggactgcct cagcggttct gcatcagata tgaggccctg
     6961 ggcacccctg gctttcacta tgttgatgtg gtgccacctc aggctaccac attcaccctg
     7021 acaggcctgc agcctagcac aagatacaga gtgtggctgc tggcctctaa tgccctggga
     7081 gattctggac tggccgacaa gggaacccag ctgcctatta caacacccgg actgcaccag
     7141 cctagcggcg aacctgaaga tcagctgcca accgagccac cttctggcgc gccggaagat
     7201 caagttgatc caagattgat tgatggcaag ggcggatctc tggaggtgct gttccaggga
     7261 ccaggcggaa gcggcaatcc cggcggcgat ctggcccccc agatgctgcg cgagctgcag
     7321 gagaccaatg ccgccctgca ggacgtgcgc gagctgctgc gccaccgcgt gaaggagatc
     7381 accttcctga agaacaccgt gatggagtgc gacgcctgcg gtatgcagcc ctctagaggc
     7441 tccggctcca tcatcccagt tgaggaggag aacccggact tctggaaccg cgaggcagcc
     7501 gaggccctgg gtgccgccaa gaagctgcag cctgcacaga cagccgccaa gaacctcatc
     7561 atcttcctgg gcgatgggat gggggtgtct acggtgacag ctgccaggat cctaaaaggg
     7621 cagaagaagg acaaactggg gcctgagata cccctggcca tggaccgctt cccatatgtg
     7681 gctctgtcca agacatacaa tgtagacaaa catgtgccag acagtggagc cacagccacg
     7741 gcctacctgt gcggggtcaa gggcaacttc cagaccattg gcttgagtgc agccgcccgc
     7801 tttaaccagt gcaacacgac acgcggcaac gaggtcatct ccgtgatgaa tcgggccaag
     7861 aaagcaggga agtcagtggg agtggtaacc accacacgag tgcagcacgc ctcgccagcc
     7921 ggcacctacg cccacacggt gaaccgcaac tggtactcgg acgccgacgt gcctgcctcg
     7981 gcccgccagg aggggtgcca ggacatcgct acgcagctca tctccaacat ggacattgac
     8041 gtgatcctag gtggaggccg aaagtacatg tttcgcatgg gaaccccaga ccctgagtac
     8101 ccagatgact acagccaagg tgggaccagg ctggacggga agaatctggt gcaggaatgg
     8161 ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca ctgagctcat gcaggcttcc
     8221 ctggacccgt ctgtgaccca tctcatgggt ctctttgagc ctggagacat gaaatacgag
     8281 atccaccgag actccacact ggacccctcc ctgatggaga tgacagaggc tgccctgcgc
     8341 ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg agggtggtcg catcgaccat
     8401 ggtcatcatg aaagcagggc ttaccgggca ctgactgaga cgatcatgtt cgacgacgcc
     8461 attgagaggg cgggccagct caccagcgag gaggacacgc tgagcctcgt cactgccgac
     8521 cactcccacg tcttctcctt cggaggctac cccctgcgag ggagctccat cttcgggctg
     8581 gcccctggca aggcccggga caggaaggcc tacacggtcc tcctatacgg aaacggtcca
     8641 ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg agagcgagag cgggagcccc
     8701 gagtatcggc agcagtcagc agtgcccctg gacgaagaga cccacgcagg cgaggacgtg
     8761 gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg gcgtgcagga gcagaccttc
     8821 atagcgcacg tcatggcctt cgccgcctgc ctggagccct acaccgcctg cgacctggcg
     8881 ccccccgccg gcaccaccga cgccgcgcac ccgggtggcg gaggactgaa cgacatcttc
     8941 gaggctcaga aaatcgaatg gcacgaacat caccatcacc atcaccatca ccattaaggc
     9001 cggcctgtag tcactggcac aacgcgttac ccggtaagcc aatcgggtat acacggtcgt
     9061 catactgcag acagggttct tctactttgc aagatagtct tgagtagtaa aataaataga
     9121 tagagaaaat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     9181 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     9241 gaaattgcat cacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     9301 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     9361 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     9421 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     9481 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     9541 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9601 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     9661 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     9721 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     9781 ggcatagtat aatacgacaa ggtgaggaac taaaccatga ccgagtacaa gcccacggtg
     9841 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     9901 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     9961 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
    10021 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
    10081 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
    10141 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
    10201 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
    10261 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga catccgcgcc ccgcaacctc
    10321 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
    10381 cgcacctggt gcatgacccg caagcccggt gcctgacacg tgctacgaga tttcgattcc
    10441 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
    10501 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
    10561 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    10621 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
    10681 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat taccaatgct taatcagtga
    10741 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10801 gtagataact acgatacggg agggcttacc atctggcccc agcgctgcga tgataccgcg
    10861 agaaccacgc tcaccggctc cggatttatc agcaataaac cagccagccg gaagggccga
    10921 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10981 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca tcgctacagg
    11041 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    11101 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    11161 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    11221 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    11281 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11341 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11401 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11461 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11521 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11581 attcttcctt
//
