LOCUS       sequence-459099-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  TITLE     other plasmids
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVTRE3G eGFP Neo (w821-1)
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2220..2446
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2447..2627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2674..2799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3292..3525
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3710..3754
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4021..4138
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4165..4543
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    4173..4191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4209..4227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4245..4263
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4281..4299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4317..4335
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4353..4371
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4389..4407
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4452..4476
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4589..4613
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4642..4694
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      4706..4715
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4712..4714,4715..4717,4718..5431)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4712..4714,4715..4717,4718..5428)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4757..4778)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5018..5037)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5365..5386
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5456..5480)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5511..6099
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5564..5584)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5982..5993)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6142..6641
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6177..6200)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6574..6593
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6655..7458
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6718..6737)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7328..7347
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7549..7782
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7854..7975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7891..7910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7945..7964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8015..8150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8170..8189)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8171..8189)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8199..8216)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8199..8215)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8208..8230)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8357..8785
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8444..8463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8654..8675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2641 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     2701 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     2761 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     2821 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     2881 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     2941 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     3001 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     3061 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     3121 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     3181 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     3241 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     3301 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     3361 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     3421 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     3481 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     3541 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     3601 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     3661 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     3721 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     3781 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3841 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3901 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3961 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac
     4021 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4081 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttat
     4141 cgatcacgag actagcctcg agttggcttt actccctatc agtgatagag aacgtatgaa
     4201 gagtttactc cctatcagtg atagagaacg tatgcagact ttactcccta tcagtgatag
     4261 agaacgtata aggagtttac tccctatcag tgatagagaa cgtatgacca gtttactccc
     4321 tatcagtgat agagaacgta tctacagttt actccctatc agtgatagag aacgtatatc
     4381 cagtttactc cctatcagtg atagagaacg tataagcttt aggcgtgtac ggtgggcgcc
     4441 tataaaagca gagctcgttt agtgaaccgt cagatcgcct ggagcaattc cacaacactt
     4501 ttgtcttata ccaactttcc gtaccacttc ctaccctcgt aaaccggttc gtcgactagt
     4561 ccagtgtggt ggaattctgc agatatcaac aagtttgtac aaaaaagcag gctttaaagg
     4621 aaccaattca gtcgactgga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
     4681 cgggcccggg atccaccggc cggtcgccac catggtgagc aagggcgagg agctgttcac
     4741 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4801 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     4861 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     4921 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4981 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5041 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     5101 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     5161 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     5221 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     5281 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     5341 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     5401 cactctcggc atggacgagc tgtacaagta aagcggccgc actcgagata tctagaccca
     5461 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgac aatcaacctc
     5521 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5581 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5641 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5701 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5761 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5821 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5881 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     5941 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6001 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6061 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattctg cagatatccg
     6121 gactagtgat ctaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     6181 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     6241 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     6301 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     6361 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     6421 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     6481 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     6541 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     6601 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc caatatggga
     6661 tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     6721 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6781 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6841 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     6901 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     6961 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     7021 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     7081 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     7141 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     7201 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     7261 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     7321 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     7381 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     7441 cttgacgagt tcttctgagg ggatccgtcg agggcccggt acctttaaga ccaatgactt
     7501 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag
     7561 ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7621 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7681 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7741 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     7801 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     7861 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7921 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7981 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8041 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8101 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     8161 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     8221 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8281 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     8341 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8401 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8461 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8521 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     8581 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8641 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     8701 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     8761 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat t
//
