LOCUS       sequence-459220-        8352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of poly H35S SUMO1 protein under T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Kim J, Qin S, Zhou HX, Rosen MK
  TITLE     Surface Charge Can Modulate Phase Separation of Multidomain 
            Proteins.
  JOURNAL   J Am Chem Soc. 2024 Feb 7;146(5):3383-3395. doi: 
            10.1021/jacs.3c12789. Epub 2024 Jan 23.
  PUBMED    38262618
REFERENCE   2  (bases 1 to 8352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.3c12789"; journalName: "J Am Chem Soc"; date: 
            "2024-02-7- 7"; volume: "146"; issue: "5"; pages: "3383-3395"
COMMENT     Sequence Label: poly H35S SUMO1
FEATURES             Location/Qualifiers
     source          1..8352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      658..667
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     775..798
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             850..861
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             874..894
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2542..2562
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2563..2580
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2593..2610)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2593..2609)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2602..2624)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2788..2805)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2958..3044
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3136..3163
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3182..3273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3274..3342,3343..4134)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3492..3511)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4176..4689)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4371..4392)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4583..4602
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      4800..5388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5289..5308
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5542..5559
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5665..5684)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5825..5847
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5841..6032)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        6528..6605
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6606..7688
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6625..6644)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(7931..7936,7937..7952,7953..7959)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    7967..7983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7972..7994
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             join(8053..8352,1..801)
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
ORIGIN
        1 aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa
       61 gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg
      121 aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg
      181 ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa
      241 gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt
      301 aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa
      361 ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa
      421 gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt
      481 ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc
      541 ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg
      601 ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc
      661 accatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc
      721 tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa
      781 gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac
      841 aacctcggga tcgagggaag gatttcagaa tttgaaaacc tgtattttca gggccatatg
      901 ggaggcggct caggtggttc tatgtccgat caagaggcaa aaccaagcac cgaggactta
      961 ggagacaaaa aggagggaga atatattaag ctgaaggtaa tcgggcagga ttcgtcagag
     1021 atcagcttta aagtgaaaat gacaactcat ttgaagaagt taaaggagtc ttactgccag
     1081 cgtcagggtg ttcctatgaa cagcctccgg ttcctctttg agggacagcg gatcgccgat
     1141 aatcacacgc cgaaggagtt ggggatggaa gaagaagatg taatcgaggt ctaccaggag
     1201 cagacagttg taggtggctc cggtggaagc gggggtagtg gtggctctat gtcggaccaa
     1261 gaagcgaagc cttcgaccga ggatttgggc gataagaagg agggtgagta tattaagctc
     1321 aaagtgatcg gacaggatag ctctgaaatt tccttcaaag tcaaaatgac tacacacttg
     1381 aaaaaactca aggaaagcta ctgtcagcgg caaggcgtcc caatgaacag tttgcgtttc
     1441 cttttcgaag gccagcggat tgccgataac cacacaccta aggaacttgg catggaggaa
     1501 gaagatgtca tcgaagtcta tcaagaacag acagtcgtgg gcggtagcgg tgggagtggg
     1561 ggatcaggcg gaagcatgtc tgaccaagaa gctaaaccgt ccactgaaga tttgggggac
     1621 aaaaaggaag gtgaatatat caagttaaag gttatcggcc aagattcctc ggagatttcg
     1681 ttcaaagtaa aaatgacaac ccacctcaaa aagttgaaag aatcttattg tcaacgtcaa
     1741 ggggtcccaa tgaattcact tcgctttctt tttgagggac aacgtatcgc ggacaaccat
     1801 acccctaaag aactcggaat ggaagaagag gacgtgatcg aggtatacca agaacaaaca
     1861 gttgtcggag gctcgggggg atctggtggt agtggaggat caatgagtga tcaggaagca
     1921 aaaccatcga ctgaggatct cggtgacaag aaagaggggg agtacattaa gcttaaggta
     1981 attggacagg actctagtga gatctccttt aaggttaaga tgacgacgca cctgaagaaa
     2041 ttaaaagagt cctactgcca acggcagggt gtgcctatga atagccttcg ttttctgttc
     2101 gagggtcaac gcatcgctga caatcatact ccaaaggagc tcggaatgga agaggaagac
     2161 gtgattgaag tttaccagga acagacggtc gttggcgggt caggcggatc gggtggttcc
     2221 ggaggctcaa tgtcagacca ggaggctaaa cctagtaccg aagaccttgg ggataaaaaa
     2281 gaaggcgaat acatcaaact gaaagtgatt gggcaagact ccagcgaaat ctcattcaag
     2341 gtcaagatga ctactcatct caaaaaactt aaagagagtt attgccaacg ccagggcgta
     2401 ccgatgaact ccctgcggtt cttgtttgaa ggccaacgta tcgccgacaa ccatacaccg
     2461 aaagagttag gtatggaaga agaagatgtt attgaggtgt accaagagca gaccgtggta
     2521 ggcggctcct gtggtggatc cgaaaacctg tattttcagg gccatcatca tcatcatcac
     2581 tgaaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     2641 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     2701 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc agcttggctg
     2761 ttttggcgga tgagataaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
     2821 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
     2881 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
     2941 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     3001 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     3061 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
     3121 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
     3181 ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3241 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3301 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3361 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3421 aacagcggta agatccttga gagttttcgc cccgaagaac gtttcccaat gatgagcact
     3481 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     3541 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3601 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3661 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3721 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3781 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3841 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3901 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3961 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4021 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4081 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4141 gaccaagttt actcatatat actttagatt gatttacccc ggttgataat cagaaaagcc
     4201 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa
     4261 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     4321 aaatccctta taaatcaaaa gaatagcccg agatagggtt gagtgttgtt ccagtttgga
     4381 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     4441 agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc
     4501 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     4561 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     4621 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     4681 agggcgcgta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4741 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4801 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4861 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4921 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4981 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5041 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5101 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5161 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5221 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5281 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5341 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5401 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5461 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5521 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     5581 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatgtggtg cactctcagt
     5641 acaccgcatg tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     5701 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     5761 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5821 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     5881 ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac agatgtctgc ctgttcatcc
     5941 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     6001 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     6061 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     6121 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     6181 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
     6241 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
     6301 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     6361 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     6421 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     6481 acgatcatgc gcacccgtgg ccaggaccca acgctgcccg aaattccgac accatcgaat
     6541 ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg
     6601 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
     6661 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
     6721 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
     6781 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
     6841 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
     6901 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
     6961 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
     7021 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
     7081 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
     7141 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
     7201 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
     7261 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
     7321 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
     7381 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
     7441 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     7501 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     7561 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     7621 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     7681 ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcaca attctcatgt
     7741 ttgacagctt atcatcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg
     7801 aagctgtggt atggctgtgc aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc
     7861 actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg
     7921 aaatgagctg ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa
     7981 caatttcaca caggaaacag ccagtccgtt taggtgtttt cacgagcact tcaccaacaa
     8041 ggaccataga ttatgaaaac tgaagaaggt aaactggtaa tctggattaa cggcgataaa
     8101 ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc
     8161 accgttgagc atccggataa actggaagag aaattcccac aggttgcggc aactggcgat
     8221 ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg
     8281 ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat
     8341 gccgtacgtt ac
//
