LOCUS       sequence-459221-        8349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of polySUMO1 protein under T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Kim J, Qin S, Zhou HX, Rosen MK
  TITLE     Surface Charge Can Modulate Phase Separation of Multidomain 
            Proteins.
  JOURNAL   J Am Chem Soc. 2024 Feb 7;146(5):3383-3395. doi: 
            10.1021/jacs.3c12789. Epub 2024 Jan 23.
  PUBMED    38262618
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.3c12789"; journalName: "J Am Chem Soc"; date: 
            "2024-02-7- 7"; volume: "146"; issue: "5"; pages: "3383-3395"
COMMENT     Sequence Label: polySUMO1
FEATURES             Location/Qualifiers
     source          1..8349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      660..669
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     777..800
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             852..863
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             876..896
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2541..2561
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2562..2579
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2592..2609)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2592..2608)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2601..2623)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2787..2804)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2957..3043
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3135..3162
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3181..3272
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3273..3341,3342..4133)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3491..3510)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4175..4688)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4370..4391)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4582..4601
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      4799..5387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5288..5307
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5541..5558
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5664..5683)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5824..5846
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5840..6031)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        6527..6604
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6605..7687
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6624..6643)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(7930..7935,7936..7951,7952..7958)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    7966..7982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7971..7993
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             join(8052..8349,1..803)
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
ORIGIN
        1 acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg atttataaca
       61 aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac
      121 tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc
      181 cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag tacgacatta
      241 aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg gttgacctga
      301 ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct gcctttaata
      361 aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc gacaccagca
      421 aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg
      481 ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg gcaaaagagt
      541 tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa gacaaaccgc
      601 tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca cgtattgccg
      661 ccaccatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt
      721 tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag actgtcgatg
      781 aagccctgaa agacgcgcag actaattcga gctcgaacaa caacaacaat aacaataaca
      841 acaacctcgg gatcgaggga aggatttcag aatttgaaaa cctgtatttt cagggccata
      901 tgggaggcgg ctcaggtggt tctatgagtg atcaggaagc caaaccctca acggaggatc
      961 ttggtgataa aaaagaaggg gagtatatta aactgaaagt gattggacag gacagtagcg
     1021 agattcattt caaagttaag atgactacgc atctgaaaaa gctgaaggaa tcgtattgcc
     1081 agcgccaggg tgttccgatg aacagcttac gcttcctgtt cgaaggccag cgcatcgcgg
     1141 acaaccatac tcctaaagag ctgggtatgg aagaggagga cgtgattgaa gtataccagg
     1201 aacagaccgt cgtaggcggc tcaggtggaa gtggcgggtc tggggggtcc atgtctgatc
     1261 aggaagctaa gccatctacc gaagatttag gtgataaaaa agagggagaa tatatcaaat
     1321 taaaagttat tgggcaggat agtagcgaga ttcattttaa agttaaaatg acgacccact
     1381 tgaaaaaact caaagaatcc tattgccaac gccagggcgt gccgatgaat agtttacgtt
     1441 ttctgtttga aggacagcgt atcgctgata accatactcc aaaagagctg ggcatggaag
     1501 aggaggacgt gatcgaggtc tatcaggagc aaacggtagt tggtggcagc ggcggcagcg
     1561 ggggctctgg cggaagcatg tcggaccaag aggcaaaacc gagtacggaa gatttggggg
     1621 ataaaaaaga aggggaatat attaagctga aagtcatcgg acaggactcg tccgaaattc
     1681 attttaaagt gaagatgacc acccatctga aaaaattaaa ggagtcatac tgtcaacgtc
     1741 aaggtgtgcc gatgaacagt ctgcgttttc tgtttgaagg ccagcgcatt gcagataacc
     1801 atacccccaa agagctgggc atggaagaag aagatgtcat cgaagtgtat caagagcaga
     1861 ctgtcgtcgg cggaagcgga ggctccggtg gcagcggtgg ttccatgtca gaccaagaag
     1921 ctaagccatc cacagaagac ctgggtgata aaaaagaagg tgagtacatt aaactgaaag
     1981 tcattggcca ggattcctcg gagatccatt ttaaagtgaa aatgaccaca cacttgaaaa
     2041 agctgaaaga gagttattgt caacgtcaag gggttccaat gaactccctt cgctttctct
     2101 tcgaaggtca acgcatcgct gataaccata ctcctaagga attaggtatg gaagaggaag
     2161 atgtaatcga agtttatcaa gaacaaaccg tggtgggcgg aagcggcggg agcggtggct
     2221 caggtggaag catgagcgat caggaggcga aacctagcac cgaggacctg ggtgacaaaa
     2281 aagagggaga atatattaag ctgaaagtta ttggtcagga cagcagtgaa attcacttta
     2341 aagtgaaaat gacgacgcac cttaaaaaac tgaaagaaag ttactgccag cgccaggggg
     2401 taccgatgaa tagtctgcgc ttcctgtttg aggggcagcg tattgcggat aatcacaccc
     2461 cgaaagagct cggcatggaa gaggaggatg tcatcgaggt atatcaggaa cagacagttg
     2521 tcggcggaag cggtggatcc gaaaacctgt attttcaggg ccatcatcat catcatcact
     2581 gaaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2641 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2701 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggca gcttggctgt
     2761 tttggcggat gagataagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     2821 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     2881 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     2941 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     3001 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     3061 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     3121 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     3181 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3241 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3301 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3361 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3421 acagcggtaa gatccttgag agttttcgcc ccgaagaacg tttcccaatg atgagcactt
     3481 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     3541 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3601 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3661 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3721 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3781 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3841 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3901 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3961 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4021 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4081 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4141 accaagttta ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc
     4201 caaaaacagg aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa
     4261 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4321 aatcccttat aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa
     4381 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4441 gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg
     4501 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4561 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4621 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4681 gggcgcgtaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4741 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4801 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4861 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4921 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4981 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5041 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5101 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5161 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5221 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5281 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5341 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5401 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5461 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5521 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     5581 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catgtggtgc actctcagta
     5641 caccgcatgt ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     5701 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     5761 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     5821 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc
     5881 tgcggtaaag ctcatcagcg tggtcgtgca gcgattcaca gatgtctgcc tgttcatccg
     5941 cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca
     6001 tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt
     6061 tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg
     6121 atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc
     6181 gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg
     6241 ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg
     6301 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg
     6361 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt
     6421 cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca
     6481 cgatcatgcg cacccgtggc caggacccaa cgctgcccga aattccgaca ccatcgaatg
     6541 gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt
     6601 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac
     6661 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga
     6721 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa
     6781 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat
     6841 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt
     6901 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt
     6961 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc
     7021 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat
     7081 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca
     7141 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc
     7201 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga
     7261 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc
     7321 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga
     7381 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag
     7441 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
     7501 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     7561 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
     7621 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     7681 gcagtgagcg caacgcaatt aatgtaagtt agctcactca ttaggcacaa ttctcatgtt
     7741 tgacagctta tcatcgactg cacggtgcac caatgcttct ggcgtcaggc agccatcgga
     7801 agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc tcaaggcgca
     7861 ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca aatattctga
     7921 aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac
     7981 aatttcacac aggaaacagc cagtccgttt aggtgttttc acgagcactt caccaacaag
     8041 gaccatagat tatgaaaact gaagaaggta aactggtaat ctggattaac ggcgataaag
     8101 gctataacgg tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca
     8161 ccgttgagca tccggataaa ctggaagaga aattcccaca ggttgcggca actggcgatg
     8221 gccctgacat tatcttctgg gcacacgacc gctttggtgg ctacgctcaa tctggcctgt
     8281 tggctgaaat caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg
     8341 ccgtacgtt
//
