LOCUS       sequence-459802-       15287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CVS N2c RVdG genome plasmid, utilized to generate negative control 
            virus to benchmark the utility of MS2 tagging for capture of 
            barcodes using snRNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15287)
  AUTHORS   Shin D, Urbanek ME, Larson HH, Moussa AJ, Lee KY, Baker DL, 
            Standen-Bloom E, Ramachandran S, Bogdanoff D, Cadwell CR, Nowakowski
            TJ
  TITLE     High-Complexity Barcoded Rabies Virus for Scalable Circuit Mapping 
            Using Single-Cell and Single-Nucleus Sequencing.
  JOURNAL   bioRxiv [Preprint]. 2024 Dec 11:2024.10.01.616167. doi: 
            10.1101/2024.10.01.616167.
  PUBMED    39713304
REFERENCE   2  (bases 1 to 15287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Dec 11:2024.10.01.616167. doi: 
            10.1101/2024.10.01.616167."
COMMENT     Sequence Label: CVS-N2c-RVdG-MCP:oScarlet-KASH_NoMBS
FEATURES             Location/Qualifiers
     source          1..15287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    43..164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(80..99)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     134..153
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(213..229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(213..229)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(226..248)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    237..253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(261..267,268..285,286..291))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    306..327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(444..461)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(615..1203)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(695..714)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1374..2165,2166..2234))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1997..2016
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2235..2339)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2414..2433)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2605..2984
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2985..3188
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3139..3159
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3233..3252
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3233..3251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6641..6650
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9701..9712
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(11504..11515)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(14714..14734)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     terminator      14905..14952
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(14961..14981)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(14966..15287,1..8)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      15146..15281
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     15208..15227
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 tttgcaaaaa gcgatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
       61 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      121 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
      181 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      301 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      361 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      421 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      481 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      541 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      601 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      661 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      721 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      781 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      841 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      901 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      961 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1021 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1081 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1141 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1201 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1261 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1321 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1381 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1441 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1501 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1561 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1621 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1681 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1741 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1801 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1861 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1921 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1981 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2041 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2101 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2161 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     2221 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2281 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2341 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     2401 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     2461 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     2521 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     2581 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     2641 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2701 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2761 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2821 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2881 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2941 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     3001 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     3061 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     3121 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     3181 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
     3241 ctcactatag ggataataga cccaagctgg ctagattaag cgtctgatga gtccgtgagg
     3301 acgaaacccg gcgtaccggg tcacgcttaa caacaaaacc agagaagaaa aagacagcgt
     3361 caattgcaaa gcaaaaatgt aacaccccta caatggatgc cgacaagatt gtgttcaaag
     3421 tcaataatca ggtggtctct ttgaagcctg agattatcgt ggatcaatat gagtacaagt
     3481 accctgccat caaggatttg aaaaagcctt gtatcaccct agggaaagcc cccgacttga
     3541 acaaagcata caaatcagtt ttatcaggca tgaatgccgc caaacttgat ccggatgatg
     3601 tatgctccta cttggcagca gcaatgcagt tctttgaggg gacatgtccg gaagactgga
     3661 ccagctatgg aatcctgatt gcacgaaaag gagataggat caccccaaac tctctagtgg
     3721 agataaagcg tactgatgta gaagggaatt gggctctgac aggaggcatg gaattgacaa
     3781 gggaccccac tgtctctgaa catgcatctt tagtcggtct tctcctgagt ctgtacaggt
     3841 tgagcaaaat atcaggacag aacactggta actataagac aaacattgca gataggatag
     3901 agcagatttt cgagacagca ccttttgtta agatcgtgga acaccatacc ctaatgacaa
     3961 ctcacaagat gtgtgctaat tggagtacta taccgaactt cagatttttg gccggaacct
     4021 acgacatgtt tttctcacgg attgagcatc tatattcggc aatcagagtg ggcacagtcg
     4081 tcaccgctta tgaagactgc tcaggactgg tatcgtttac agggttcata aagcagatca
     4141 atctcaccgc aagggaagca atactatatt tcttccacaa gaactttgag gaagagataa
     4201 gaagaatgtt cgagccaggg caagagacag ctgttcctca ctcttatttc atccacttcc
     4261 gttcactagg cttgagtggg aagtctcctt attcatcgaa tgctgtcggt catgtgttca
     4321 atctcattca ctttgttgga tgctacatgg gtcaagtcag atctctaaat gcgacggtta
     4381 ttgctgcatg tgcccctcat gagatgtctg ttctaggggg ctatttggga gaggaattct
     4441 tcggaaaagg gacatttgaa agaaggttct tcagagacga gaaagaactt caagaatatg
     4501 aggcggctga actaacaaag tccgacgtgg cactggcaga tgacggaacc gtcaactctg
     4561 atgacgagga ctatttctct ggtgaaacca gaagtccaga agctgtctat actcgaatca
     4621 tgatgaatgg aggtcgactg aagagatctc atatacggag atatgtctca gtcagttcca
     4681 atcatcaagc ccgtccaaac tcattcgccg aatttttaaa caagacgtat tcgaatgact
     4741 cataaggagt tgattgacag ggtgccagaa atctatagat tgtatatatc catcatgaaa
     4801 aaaactaaca ctcctccttt caaaccatcc caaatatgag caagatcttt gttaatccga
     4861 gtgcaatcag agccggtctg gccgatcttg agatggccga agagactgtt gatctgatca
     4921 acagaaacat agaagacaat caggctcatc tccagggaga acccatagaa gtggacaacc
     4981 tccctgagga catgaagcga cttcacctgg acgatgaaaa atcgtccaac cttggtgaga
     5041 tggttagggt gggagaaggc aagtatcgag aggactttca gatggatgag ggagaggacc
     5101 ccaacctcct gttccaatcg tacctggata atgttggagt ccaaatagtc agacaaatga
     5161 ggtcaggaga gagattcctc aagatatggt cacagaccgt agaggaaatt gtatcctatg
     5221 tcacggtcaa ctttcctaac cctccaagaa ggtcttcgga ggataaatca acccagacta
     5281 ctggcagaga gctcaagaag gagacaacgt ctgctttctc tcagagagaa agccaacctt
     5341 cgaaagctag gatggtggct caagttgccc ctggtcctcc agcccttgaa tggtcagcca
     5401 ccaatgaaga agatgatcta tcagtagagg ctgagatcgc tcatcagatt gctgaaagct
     5461 tttccaagaa gtacaagttt ccctcccgat cttcaggaat attcttgtat aattttgagc
     5521 aactgaagat gaaccttgat gacatagtta aagaggcaaa aaatgtaccg ggcgtgaccc
     5581 gtctggccca tgatggatcc aaaatccccc tgagatgcgt actgggatgg gtcgctttgg
     5641 ctaattccaa aaaattccaa ttactagtcg aggctgacaa gctaagcaaa atcatgcaag
     5701 atgatttgaa tcgctataca tcctgctaac cgagttttcg aactcagtcc ctccagataa
     5761 tgaaaactga gatgttatgg agtagacatg aaaaaaacag gcaacaccac tgataaaatg
     5821 aacgttctac gcaagatagt gaaaaaatgt agggatgagg acactcaaaa gccctctcct
     5881 gtgtcagccc ctccgtatga cgatgacctg tggcttccac ctcctgaata tgtcccgctg
     5941 aaagaactca caagcaagaa gaacatgagg aacttttgtg tcaacgggga ggttaaagcg
     6001 tgtagcccaa atggttactc attcaggatt ttgcggcaca ttctgagatc attcaacgag
     6061 atatactctg ggaatcatag gatgattggg ttagtcaaaa ttgttgttgg actagcttta
     6121 tcaggagctc cagtacctga gggcatgaac tgggtataca aattgaggag aacccttata
     6181 ttccagtggg ctgattccag gggccctctt gaaggggagg agttagatta ctctcaagag
     6241 atcacttggg atgatgatac tgaattcgtc ggattgcaaa taagagtgag cgcaagacaa
     6301 tgtcatattc aaggcaggat ctggtgtatc aacacgaact cgagggcatg tcaactatgg
     6361 tctgacatgt ctcttcagac acaaaggtct gaagaggaca aagactcttc tctgcttcta
     6421 gaataatcag attatatcct gcaagtgtat cacttgttta cctctggagg agagagcata
     6481 caggcttgac tccgatcctt gggagcaata gaacaaaaaa acacacgtta tggtgccgtt
     6541 aaatcgctgc attttatcaa agtcaagttg ataaccttta cattttgagc ctcttggatg
     6601 tgaaaaaaac tattaacatc cctcaaaaga ccccgggaaa gccaccatgg cttctaactt
     6661 tactcagttc gttctcgtcg acaatggcgg aactggcgac gtgactgtcg ccccaagcaa
     6721 cttcgctaac gggatcgctg aatggatcag ctctaactcg cgttcacagg cttacaaagt
     6781 aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa tacaccatca aagtcgaggt
     6841 gcctaaaggc gcctggcgtt cgtacttaaa tatggaacta accattccaa ttttcgccac
     6901 gaattccgac tgcgagctta ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc
     6961 gattccctca gcaatcgcag caaactccgg catctacgga ggatcgggcg gaatggtgag
     7021 caagggcgaa gccgttatta aggaatttat gcgttttaaa gttcatatgg aaggttctat
     7081 gaatgggcat gaatttgaaa ttgaagggga aggagaaggt cggccatatg aaggaacaca
     7141 aacggctaaa ctcaaagtca ctaaaggcgg accattgccg ttttcttggg atatcttgag
     7201 cccacaattt atgtatggta gtcgcgcatt tacgaaacat ccagcagata tacctgatta
     7261 ttacaaacaa tcatttcctg aaggatttaa atgggatcgg gttatgaatt ttgaagatgg
     7321 tggtgcagtt acggtaacac aagatacttc acttgaagat ggtacactta tttataaagt
     7381 caaactccgg ggtacaaatt ttcccccaga tggacctgtc atgcaaaaga aaactatggg
     7441 ttgggaagcc tcaactgaaa gactttatcc tgaagatggt gtcctcaaag gggatatcaa
     7501 aatggctttg agattaaaag atggtggccg gtatcttgcc gattttaaaa ctacctataa
     7561 agccaagaaa ccagtccaaa tgccaggtgc atataatgtt gaccggaaat tagatattac
     7621 atcccacaat gaagattaca cagttgtaga gcaatatgag cggagcgaag gtagacattc
     7681 tactggcgga atggatgaac tgtacaagtc cggtttgagg tcaagagaag aagaggagga
     7741 gactgactcc cgaatgccgc acttggactc accagggagt agtcagccta gacgcagttt
     7801 tctgagccgc gtgataagag ccgccctgcc ccttcagttg ctgcttctcc tgcttctctt
     7861 gctcgcgtgt ctgctccccg catctgaaga tgattactcc tgtacccagg ctaacaactt
     7921 cgcacgatca ttttatccta tgttgagata taccaacgga ccccctccta cataagcggt
     7981 agcttttcag tcgagaaaaa aactgtagac caaaagaaca actagcaaca cttctcatcc
     8041 agagacccat atcaagatgc tagatccggg agaggtttat gatgacccta ttgatccaat
     8101 tgagtcagag gctgaaccca gaggaacccc cactgtcccc aacatcttga ggaactccga
     8161 ctacaatctc aattctcctt tgatagagga tcctgccaaa ctaatgttag aatggttgaa
     8221 gacagggaac agaccttatc ggatgacttt gacagacaat tgctccaggt cttacaaagt
     8281 tttgaaagat tatttcaaga aagtagattt gggttctctc aaagtgggcg gaactgctgc
     8341 acagtcaatg gtttctctct ggttgtgtgg tgcccactct gaatcaaaca ggagccggag
     8401 atgtataacc gacttggccc atttctattc caagtcatcc cccatagaga agctattgaa
     8461 ttgtacgcta ggaaacagag gcctgagaat cccaccagag ggggtgttaa attgcctcga
     8521 gagggtcaat tatgacaagg catttgggag gtatctggcc aacacgtatt cctcttactt
     8581 gtttttccat gtaatcacct tatacatgaa tgccttagac tgggaagagg aaaagaccat
     8641 cctggcatta tggaaagata taacctcagt ggataccgag aaggacttgg tcaaattcaa
     8701 agatcaaata tggggactgt tgattgtgac aaaggacttt gtttactctc agagttcaaa
     8761 ctgtcttttt gacagaaact acacactgat gctaaaggat cttttcttgt ctcgattcaa
     8821 ctccttaatg attctgcttt ctccccctga gccccgatac tcagatgact taatatctca
     8881 gctgtgccag ctatacatcg ctggggatca agtcttgtcc atgtgtggga actccggcta
     8941 tgaagtcatc aaaatattgg agccatatgt cgtgaacagt ttggtccaga gggcagagaa
     9001 gtttaggcct ctcatccacc ccttgggaga ctttcctatg ttcataaaag acaaggtgaa
     9061 tcaacttgaa gggacttttg gtcccagtgc aaagaggttt tttagggttt tagatcaatt
     9121 cgacaacata catgacttag tatttgtgta tggctgttac agacattggg ggcaccccta
     9181 tatagattat cggaagggtc tgtcgaaact atatgatcaa gttcacatta agaaagtaat
     9241 agataagtcc taccaggagt gtttagcaag tgacttggcc agaaggatcc tcagatgggg
     9301 atttgacaag tactccaaat ggtatctaga ttcgagattc cttgcccgag accacccctt
     9361 gactccttat gtcaagaccc aaacatggcc acccaaacat atagtagact tggtggggga
     9421 cacatggcat aagctcccga tcacgcagat ctttgaaatt cctgaatcaa tggacccgtc
     9481 agagatactg gatgacaaat cacattcttt caccagaaca aggttagctt cttggctgtc
     9541 agagaaccga ggggggcctg ttcctagcga gaaggtcatt atcacggccc tgtctaagcc
     9601 acctgtcaat ccccgagagt ttttgaaatc tatagacctc ggaggattgc cagatgagga
     9661 tttgataatt ggcctcaaac caaaggaacg ggagttgaag atcgagggcc gattctttgc
     9721 tctaatgtca tggaatctaa gattatattt tgtcatcacc gaaaagctct tggccaacta
     9781 cattttgcca ctttttgacg cactgactat gacagacaac ctgaacaagg tgttcaaaaa
     9841 gttgatcgac agggtcaccg ggcaagggct tttggactat tctagggtca catacgcatt
     9901 tcacctggac tatgagaaat ggaacaatca tcaaagattg gagtcaacag aggatgtatt
     9961 ctctgtccta gatcaggtgt ttggattgaa gagggtgttt tctagaacac acgagttttt
    10021 tcagaagtcc tggatctatt attcagacag atcagacctc attgggttat gggaggacca
    10081 aatatattgc ttggatatgt ctaacggccc aacctgctgg aatggccaag atggcgggct
    10141 agagggcttg cggcagaagg gctggagtct agtcagttta ttaatgatag atagagaatc
    10201 tcaaaccagg aacacaagaa ccaagatact agctcaagga gacaaccagg ttctgtgtcc
    10261 tacatacatg ttgtcaccgg gattgtctca agaggggctt ctctatgagt tagagagcat
    10321 atcaaggaat gcactctcaa tataccgagc tatcgaggaa ggagcatcta agctggggct
    10381 gatcatcaag aaggaagaga ccatgtgtag ttatgacttt ctcatatatg ggaagacccc
    10441 cttatttcga ggcaacatat tggtgcctga atccaaaaga tgggcccgag tctcttgcat
    10501 ctctaacgac caaatagtca acctcgccaa tataatgtcg acagtatcca ccaatgcgct
    10561 gacagtggca caacactctc aatctctgat caaacctatg agggattttc tgctcatgtc
    10621 agtacaggca gttttccact acctgttgtt tagcccaatc ctaaaaggca gagtttataa
    10681 gattctgagt gctgaagggg agagctttct cctagccatg tcgcggataa tctacctaga
    10741 tccttctttg ggaggggtgt ctggaatgtc tctcgggagg ttccatatac gtcagttctc
    10801 agaccctgtc tctgaagggt tgtcattctg gagagagatc tggttaggct ctcatgagtc
    10861 ctggattcac gcgttgtgtc aggaggccgg gaaccccgat cttggagaga gaacactcga
    10921 gagcttcact cgccttttag aagatcctac taccttaaat atcaaaggag gggccagccc
    10981 taccattcta ctcaaggatg ctatcagaaa ggctctgtac gacgaggtgg acaaggtgga
    11041 aaattcagag tttcgagagg caatcctgct gtccaagacc catagagata actttatact
    11101 ctttttaaaa tctgttgagc ctctgttccc tcgatttctc agtgagctct tcagttcgtc
    11161 tttcttggga ataccggagt caatcattgg actgatacaa aactcccgga caataagaag
    11221 gcagtttaga aagagtctct caagaacttt agaagagtcc ttctacaact cagagatcca
    11281 cgggattaat cggataaccc agacacctca aagggtcgga agggtgtggc cttgctcttc
    11341 agagagggca gatctactta gggagatctc ttgggggagg aaagtggtag gcacgacagt
    11401 tcctcaccct tccgagatgt tggagttgtt tcccaaatcc tccatttcct gcacttgtgg
    11461 agcaacaggg ggaggcaatc ctagagtctc tgtatcagta ctcccgtcct tcgatcagtc
    11521 atttttctca cggggccccc taaagggata cttgggctcg tccacctcca tgtcaaccca
    11581 gctattccat gcatgggaaa aagtcactaa tgttcatgtg gtgaaaaggg ctctatcgtt
    11641 aaaagaatct ataaactggt tcatcaatag gaattccaat ttggctcaaa ctctaattgg
    11701 aaacatcatg tctctgacag gccctgattt ccctctagaa gaggcccctg ttttcaaacg
    11761 gacagggtca gccttgcata ggttcaagtc tgccagatac agcgaaggag ggtattcttc
    11821 tgtttgccct aaccttctct cccatatctc tgttagtaca gacactatgt ctgatttgac
    11881 ccaaaacggg aagaactatg atttcatgtt tcagccattg atgctttatg cgcaaacatg
    11941 gacatcggaa ctggtacaga gggatacaag acttagagac tccacgtttc actggcacct
    12001 tcggtgcaac agatgtgtga ggcccattga tgatataaca ctggaaactt ctcagatctt
    12061 cgagttcccg gatgtgtcaa aaaggatatc caggatggtt tctggagctg tccctcaatt
    12121 tcagaagctt cctgatatcc gtctaagacc aggtgatttt gaatccctaa gtggtagaga
    12181 aaagtctcgc catatagggt cagctcaggg gctcttatac tcaatcttag tagcaattca
    12241 cgactcagga tacaatgatg ggaccatctt ccctgtcaac atatacggca aagtttcccc
    12301 cagagactat ttgagagggc ttgcaagagg gatcttgata gggtcctcga tttgcttctt
    12361 gacacgaatg acaaatatta acattaacag acctcttgaa ttgatctcag gggtaatttc
    12421 ctatattctc ctgaggctgg ataatcatcc ctctctgtat ataatgctta gagaaccgtc
    12481 tcttagagga gaaatattct ctatccctca gaaaatcccc gccgcttacc caaccactat
    12541 gagagaaggc aacagatcga ttttgtgtta cctccaacac gtgctacgct atgagcgaga
    12601 ggcaatcacg gcgtccccgg agaatgactg gctgtggatc ttctcagact tcagaagtgt
    12661 gaaaatgacg tacttgaccc tcattaccta ccagtctcac ctcctactcc agagggttga
    12721 gagaaacttg tctaagagta tgagagctac tctgcgacaa atgagttcct taatgaggca
    12781 agtgctgggt gggcacggag aagatacctt ggagtcagac gatgacattc aacgattact
    12841 aaaagactct ttgcgaaaga caaggtgggt ggatcaagag gtgcgccatg cagctagaac
    12901 catgaatgga gattacagcc ccgacaagaa agtatcccac aaggcaggat gttcagaatg
    12961 ggtctgctct gctcaacaga ttgccgtctc cacctcagcc aacccggccc ctgtctcaga
    13021 gcttgacatt agggccctct ctaagaggtt tcaaaacccc ttgatctcgg gcctgagagt
    13081 ggttcagtgg gcaaccggtg cccattataa gcttaagcct attctagatg atctaaatgt
    13141 tttcccatct ctctgtcttg taattggaga cgggtcaggg ggaatatcaa gggcagttct
    13201 caacatgttt ccagattcta agcttgtgtt caacagccta ttggaggtga atgatctgat
    13261 ggcttccgga acacatccac tgcctccttc agcaatcatg agtggaggag atgacatcat
    13321 ctccagagtg atagactttg actcaatatg ggagaaaccg tccgacctga ggaactcggc
    13381 cacctggaga tacttccagt cggttcaaaa acaggtcaac atgtcgtatg acctcattat
    13441 ttgtgatgca gaagttactg atattgcatc tatcaaccgg ataactctgt tgatgtctga
    13501 tttcgcattg tctatagatg gaccacttta tctggtcttc aaaacttacg ggactatgct
    13561 agtaaacccg gactataaag ctattcaaca tctgtcaaga gcgttccctt cggtcacagg
    13621 gtttgtaacc caagtaactt catccttttc ttctgagcta tacctccggt tctctaaacg
    13681 aggaaagttt ttcagggacg ccgagtactt gacctcttcc acccttcgag agatgagcct
    13741 tgtgttgttc aattgcagca gccccaaaag tgagatgcag agagctcgtt ccttaaacta
    13801 tcaagacctg gtaaggggat ttcctgaaga gatcatatca aatccttaca acgaaatgat
    13861 cataactctg attgacaatg atgtagagtc cttcctagtc cacaagatgg tggatgatct
    13921 tgagctacag aggggaactc tgtctaaagt ggctatcatt atatccatca tgatcgtttt
    13981 ttccaataga gtcttcaaca tttccaaacc tttgactgac cccttgttct accccccatc
    14041 tgatcctaaa atcctgaggc acttcaacat atgttgcagt actatgatgt atctatctac
    14101 cgctttaggc gacgtcccta acttcgcaag acttcatgac ctgtataaca gacctataac
    14161 ttgttacttc agaaaacaag ttattcgagg gaatatttat ctatcttgga gttggtccga
    14221 tgatacccca gtgttcaaga gagtagcctg taattctagc ttgagtctgt catctcactg
    14281 gatcaggttg atctacaaga tagtgaagac taccagactc attggcagca taaaagacct
    14341 atcaggagag gtagaacgac atcttcatgg gtataacaga tggatcaccc tcgaggatat
    14401 ccgatctaga tcatccctac tagactacag ttgcctgtga accggatact cctggaagcc
    14461 tgcccatgct aagactcttg tgtgatgtat cttgaaaaaa acaagatcct aaatctgaac
    14521 ctttggttgt ttgattgttt ttctcatttt tgttgtttat ttgttaagcg tgggtcggca
    14581 tggcatctcc acctcctcgc ggtccgacct gggcatccga aggaggacgc acgtccactc
    14641 ggatggctaa gggagagcca aatcaacctc tggattacaa aatttgtgaa agattgactg
    14701 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
    14761 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag
    14821 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
    14881 tgttgggcac tgacaattcc gtggctagca taaccccttg gggcctctaa acgggtcttg
    14941 aggggttttt tgaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
    15001 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
    15061 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    15121 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    15181 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
    15241 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggct
//
