LOCUS       sequence-459836-        9611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral control vector expressing miniTurboNLS without dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Cenik BK, Aoi Y, Iwanaszko M, Howard BC, Morgan MA, Andersen GD, 
            Bartom ET, Shilatifard A
  TITLE     TurboCas: A method for locus-specific labeling of genomic regions 
            and isolating their associated protein interactome.
  JOURNAL   Mol Cell. 2024 Dec 19;84(24):4929-4944.e8. doi: 
            10.1016/j.molcel.2024.11.007.
  PUBMED    39706164
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.11"; journalName: "Mol Cell"; date: 
            "2024-12-19- 19"; volume: "84"; issue: "24"; pages: "4929-4944.e8"
COMMENT     Sequence Label: Lenti-miniTurbo-NLS-Blast
FEATURES             Location/Qualifiers
     source          1..9611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..61
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             62..460
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    485..1073
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(538..558)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(956..967)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1076..1092)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1077..1093)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1598..1778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1804..1821)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1810..2034
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2080..2508
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2167..2186)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2377..2398
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2517..2537)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2682..2816
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2743..2762
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2878..2925
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2944..3318
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3448..3569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3485..3504)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3539..3558
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3618..3634)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3618..3634)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3631..3653)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3642..3658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3666..3672,3673..3690,3691..3696))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3711..3732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3849..3866)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4020..4608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4100..4119)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4779..5570,5571..5639))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5640..5744)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5819..5838)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6010..6389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6391..6589
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6544..6564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6607..6787
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6834..6959
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7452..7685
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7870..7914
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8212..8329
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8422..8633
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             8662..8688
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8692..8718
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8722..8748
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8755..9525
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             9532..9552
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9556..9576
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9580..9600
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cggcgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
       61 gatggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
      121 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
      181 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
      241 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
      301 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
      361 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
      421 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gaattcgata tcaagcttat
      481 cggtaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
      541 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
      601 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
      661 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
      721 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
      781 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
      841 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
      901 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
      961 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     1021 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     1081 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     1141 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     1201 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     1261 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     1321 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     1381 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     1441 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     1501 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     1561 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     1621 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1681 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1741 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     1801 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     1861 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     1921 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     1981 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     2041 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     2101 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2161 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2221 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2281 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2341 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2401 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2461 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     2521 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     2581 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     2641 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     2701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     2761 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     2821 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     2881 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     2941 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     3001 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     3061 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     3121 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     3181 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     3241 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     3301 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     3361 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     3421 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     3481 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     3541 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     3601 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3661 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3721 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3781 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3841 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3901 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3961 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4021 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4081 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4141 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4201 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4261 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4321 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4381 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4441 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4501 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4561 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4621 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4681 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4741 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4801 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4861 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4921 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4981 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5041 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5101 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5161 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5221 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5281 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5341 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5401 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5461 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5521 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5581 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5641 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5701 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5761 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     5821 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     5881 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     5941 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     6001 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     6061 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     6121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     6181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     6241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     6301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     6361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     6421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     6481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     6541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc
     6601 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6661 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6721 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6781 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     6841 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     6901 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     6961 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     7021 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     7081 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     7141 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     7201 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     7261 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     7321 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     7381 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     7441 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     7501 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     7561 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     7621 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     7681 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     7741 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     7801 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     7861 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     7921 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     7981 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     8041 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     8101 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     8161 tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag
     8221 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     8281 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     8341 cagggacagc agagatccag tttggttaat tagctagcta ggtcttgaaa ggagtgggaa
     8401 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     8461 ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa
     8521 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     8581 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggaccggt
     8641 tctagagcgg ccgccaccat gtacccgtat gatgttccgg attacgctgg ctatccctac
     8701 gacgtgcccg actatgccgg gtacccctat gacgtcccag actacgcagc tagcatcccg
     8761 ctgctgaacg ctaaacagat tctgggacag ctggacggcg ggagcgtggc agtcctgcct
     8821 gtggtcgact ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat
     8881 gcttgcattg cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct
     8941 ccttttggag ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca
     9001 atcggcctgg gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca
     9061 gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc
     9121 atcctggtgg agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg
     9181 attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg
     9241 caggaagcag ggattaacct ggacaggaat actctggccg ctatgctgat ccgagagctg
     9301 cgggcagccc tggaactgtt cgagcaggaa ggcctggctc catatctgtc acggtgggag
     9361 aagctggata acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc
     9421 gggattagcc gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc
     9481 aaaccctgga tgggcggaga aatcagtctg cggtctgccg aaaagtgtac acccaagaag
     9541 aagaggaagg tggaccccaa gaagaagagg aaggtggacc ccaagaagaa gaggaaggtg
     9601 tgtacaggat c
//
