LOCUS       sequence-459986-       16872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous production of ophiobolin C in Aspergillus oryzae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16872)
  TITLE     Aspergillus oryzae plasmids
REFERENCE   2  (bases 1 to 16872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHS201-ophi
FEATURES             Location/Qualifiers
     source          1..16872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10017..10033
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14651..14667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14664..14686)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14675..14691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14699..14705,14706..14723,14724..14729))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14744..14765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14882..14899)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15053..15641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15133..15152)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15812..16603,16604..16672))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16435..16454
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16673..16777)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     16845..16863
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accctgcact
      421 cagctttccg gaactaacgt gcaataggtg gcggtgcctg gttgggtggc caactctttt
      481 ctgctatggt catgcgccgt cccgcggaag tcttcctgaa cgagctgggt gttccttacg
      541 aagaggacgc aaaccccaac tacgttgtcg tcaagcacgc ctccctgttt acctcgacac
      601 tcatgtcgaa ggttctctcc ttccccaatg tcaagctctt caatgctacc gctgttgagg
      661 acttgatcac ccgtccgacc gagaacggca acccccagat tgctggtgtt gtcgtcaact
      721 ggacgctggt cacccttcac cacgatgatc actcctgcat ggaccccaac actatcaacg
      781 ctcctgtcat catcagtacc actggtcacg atgggccatt cggcgccttc tgtgcgaagc
      841 gcttggtgtc catgggcagc gtcgacaagc taggtggcat gcgtggtctc gacatgaact
      901 cggccgagga tgccatcgtc aagaacaccc gcgaggttac taagggcttg atcatcggcg
      961 gtatggagct gtctgaaatt gatggcttta accgcatggg ccctaccttc ggtgccatgg
     1021 ttctcagtgg tgtcaaggct gccgaggagg cattgaaggt gttcgacgag cgtcagcgcg
     1081 agtgtgctga gtaaatgact cactacccga atgggttcag tgcatgaacc ggatttgtct
     1141 tacggtcttt gacgataggg gaatgatgat tatgtgatag ttctgagatt tgaatgaact
     1201 cgttagctcg taatccacat gcatatgtaa atggctgtgt cccgtatgta acggtggggc
     1261 attctagaat aattatgtgt aacaagaaag acagtataat acaaacaaag atgcaagagc
     1321 ggctcatcgt caccccatga tagtcatact cattccaact tctgcccagt tccaccactt
     1381 ttctcgacta attctttcgc attccgccac ttgtcccgcg gcacagagcc cactacccga
     1441 atcgatagaa ctactcattt ttatatagaa gtcagaattc atggtgtttt gatcatttta
     1501 aatttttata tggcgggtgg tgggcaactc gcttgcgcgg gcaactcgct taccgattac
     1561 gttagggctg atatttacgt aaaaatcgtc aagggatgca agaccaaagt agtaaaaccc
     1621 cggagtcaac agcatccaag cccaagtcct tcacggagaa accccagcgt ccacatcacg
     1681 agcgaaggac cacctctagg catcggacgc accatccaat tagaagcagc aaagcgaaac
     1741 agcccaagaa aaaggtcggc ccgtcggcct tttctgcaac gctgatcacg ggcagcgatc
     1801 caaccaacac cctccagagt gactaggggc ggaaatttaa agggattaat ttccactcaa
     1861 ccacaaatca cagtcgtccc cggtattgtc ctgcagaatg caatttaaac tcttctgcga
     1921 atcgcttgga ttccccgccc ctggccgtag agcttaaagt atgtcccttg tcgatgcgat
     1981 gtatcacaac atataaatac tagcaaggga tgccatgctt ggaggatagc aaccgacaac
     2041 atcacatcaa gctctccctt ctctgaacaa taaaccccac agaaggcatt tcccatggcc
     2101 tgcaagtact cgacactcat cgactcctcc ctgtacgaca gggaaggtct ttgccccgga
     2161 attgatctca ggagacatgt cgccggtgag cttgaagagg tcggtgcttt cagggcccaa
     2221 gaagactggc gccgtttggt tggtcccctt ccaaagcctt atgcgggcct cttaggaccc
     2281 gactttagct tcataaccgg cgcggtgcca gagtgtcacc cagatagaat ggagatcgtc
     2341 gcttatgcgc tggagtttgg tttcatgcat gacggtatgt gctgactgtc tggagttgcc
     2401 aaacgaggct cattcaaaca gatgtcatcg atacggatgt caaccatgcc tcattggatg
     2461 aggtgggaca taccttggat caaagtcgaa ctggcaaaat cgaagacaag ggctccgatg
     2521 gaaagcgcca aatggtcact caaatcatcc gcgaaatgat ggcaattgat ccagagagag
     2581 cgatgactgt agcgaagagc tgggcctccg gcgtccgaca ttcaagcaga cggaaggagg
     2641 acacgaactt taaggcactt gagcagtata taccctacag ggccctcgac gtcgggtaca
     2701 tgctctggca cggcctggtc acctttggct gcgcaattac aattcccaac gaagaagaag
     2761 aagaggcaaa gaggctcatc atacctgcgt tagtccaagc gtcgctgctg aacgaccttt
     2821 tctccttcga gaaggaaaag aacgacgcta atgtccagaa cgctgtcttg attgtcatga
     2881 atgagcatgg gtgtagcgaa gaagaagcaa gagatatcct caagaaacgc atccgccttg
     2941 aatgtgccaa ctacctccgc aatgtcaaag agaccaatgc gcgggcggat gtcagtgatg
     3001 agttgaagag gtacatcaat gtcatgcagt ataccctttc cggcaacgca gcctggagta
     3061 cgaattgccc gcggtacaac ggaccaacca agtttaatga gttgcagttg ctgagaagcg
     3121 agcacggcct ggcaaaatac ccgtcaaggt ggtcacagga gaacagaacc agcggcctcg
     3181 ttgagggtga ttgccacgaa tccaagccaa acgagctcaa gaggaagagg aatggcgtca
     3241 gtgtagatga cgaaatgagg acgaatggca ctaatggcgc caagaagcca gcgcatgtct
     3301 cgcaaccaag cacggattcg attgttctag aggatatggt gcagttggcg cgtacttgcg
     3361 atttaccgga cttgagtgat acagtgcgtt agatgtattc ctgcgctgac gcagctgtca
     3421 ctgacggtga ttccaggtta ttctccaacc ataccggtac cttacctccc tcccctctaa
     3481 gggtttccga gaccaagcca tagactccat caataaatgg ctgaaggtgc ccccgaagtc
     3541 ggtgaagatg atcaaagacg tcgtcaagat gctgcatagt gcatctctca tgtgagtcct
     3601 tgccctcttt cttttatcgc gcttttctag ggtctgacaa ccccaggctc gatgatctcg
     3661 aagacaactc tccattacgt cgtggcaagc cctctaccca tagtatctac ggcatggccc
     3721 agacagtcaa tagcgcaacg taccaataca tcacagctac agatataacc gcccaactcc
     3781 agaactcaga aacctttcat atcttcgttg aagagttaca gcagctgcac gtggggcaga
     3841 gctacgacct ctactggacg cacaacacgc tctgcccaac catcgctgag tatttgaaaa
     3901 tggttgacat gaagacgggc ggtctatttc gcatgttgac gcggatgatg atcgccgaga
     3961 gcccggtcgt cgataaggtt cccaacagtg atatgaattt gtttagttgc ctcattggac
     4021 gcttcttcca gatccgcgac gactatcaaa atctcgcttc agctgactac gcaaaggcga
     4081 aggggttcgc cgaggatctc gacgaaggga aatattcctt cacgctgatc cactgcattc
     4141 agactctgga gtcaaagccc gagctcgcag gggagatgat gcagttgcgg gcattcctta
     4201 tgaaaagaag gcatgaaggc aaacttagcc aagaggctaa gcaagaggtg ttagtaacca
     4261 tgaagaaaac agaaagcttg caatacacgc tcagcgttct gcgggaactg cacagcgagt
     4321 tggagaagga agttgaaaat ttagaggcga agtttggcga ggagaacttc actcttagag
     4381 tgatgctaga gttgctgaag gtgtaacccg atctgtagta gctcgtgaag ggtggagagt
     4441 atatgatggt actgctattc aatctggcat tggacagtga gtttgagttt gatgtacagt
     4501 tggagtcgtt actgctgtca tccccttata ctcttcgatt gtttttcgaa ccctaacgcc
     4561 aagcacgcta gtctattata ggaaaggatc cactacccga atcgatagaa ctactcattt
     4621 ttatatagaa gtcagaattc atggtgtttt gatcatttta aatttttata tggcgggtgg
     4681 tgggcaactc gcttgcgcgg gcaactcgct taccgattac gttagggctg atatttacgt
     4741 aaaaatcgtc aagggatgca agaccaaagt agtaaaaccc cggagtcaac agcatccaag
     4801 cccaagtcct tcacggagaa accccagcgt ccacatcacg agcgaaggac cacctctagg
     4861 catcggacgc accatccaat tagaagcagc aaagcgaaac agcccaagaa aaaggtcggc
     4921 ccgtcggcct tttctgcaac gctgatcacg ggcagcgatc caaccaacac cctccagagt
     4981 gactaggggc ggaaatttaa agggattaat ttccactcaa ccacaaatca cagtcgtccc
     5041 cggtattgtc ctgcagaatg caatttaaac tcttctgcga atcgcttgga ttccccgccc
     5101 ctggccgtag agcttaaagt atgtcccttg tcgatgcgat gtatcacaac atataaatac
     5161 tagcaaggga tgccatgctt ggaggatagc aaccgacaac atcacatcaa gctctccctt
     5221 ctctgaacaa taaaccccac agaaggcatt tatgagtttg ggacccggtg gcttcgaggc
     5281 tacccccaac agtgtgatgc cttccgacgc ttcactacac aatcagtccc accacaccga
     5341 cagcctcaca aacaacgaca gcatcgccag taccgaacag agggaaaaag aggtccatca
     5401 gctcgccagg aagtacacac aaaacagcgt ctactcaact accagccaga acccttttgc
     5461 agctgaacct ggatctaaac ttgaccccaa tggcggcaat ttcagtgcgc gcgcttgggc
     5521 aaaggccatg ctgcagacac ataccgacga caaccaggca catcctctcc gcacactagg
     5581 tgtatccttt agcaacctga atgtgtacgg tttcggattc gacaccgact accagaagag
     5641 cgttggcaac atctggctag aggcggtcgg cctcgtcagg aagctcatgg gccaacgcga
     5701 gcgcaagatc gagatcctac gcgatttgga gggactggtc gaggctggag agatgcttgt
     5761 cgtccttggt cctcccggag caggatgctc aaccttcttg aagaccctta ctggacaaac
     5821 tcacggcttc tacgtcgatg ataaatcgaa cctcaattac caaggtaagg cttagatgcc
     5881 ttttgaactc gctgcctccc actgacacgc ttcaggtgtg actcccaagc agctgatcaa
     5941 gaacttccga ggagaagcca tctataccgc tgaagtcgac gttcacttcc ccaacatcac
     6001 tgttggagat actcttttct tcgctgctcg cgcgcgagcc ccgcgtcata ttcctggagg
     6061 cgctaccatc gaccagtatg ccgaacacat gcgcgacgtc attatggcca gcttcggcat
     6121 cagccacacg aagaacacca ttgtcggaaa cgatttcatt cgcggagtat ctggaggaga
     6181 gcgcaagcgt gtcagtattt ccgaagcctg tctcagccag gcgccgctgc aatgttggga
     6241 caactcgacc cgtggtcttg acagcgccaa tgctatcgag ttttgcaaga cgctacgcat
     6301 gcaaaccgaa atcaacggag ctaccgcctg tgttgccatc taccaagcac ctcaagctgc
     6361 ctacgactac ttcgacaaag tactggtttt gtacaagggt cgccagattt actttggccc
     6421 taccgcccag gccaagcagt acttcttgaa catgggtttc gtctgccccg atcgacaaac
     6481 cgatgccgat ttcctcactt ccatgaccag ccatcttgag cgtgttgtgc agcctgggta
     6541 tgagaaccag gttccgcgaa cgccagatga gttcgctgct cggtggaagg cctcgcgcga
     6601 acgcgcagag ctcctgaacc agattgagat gtacaactcg aaatacgcaa ccggtggcga
     6661 gcatctggaa cgattcaaag aatcccgacg agcccagcaa gccaaggccc agcgtgtatc
     6721 gtcgccgtat actctgtcat acactcagca aatcaagctg tgcctctggc gcagctgggt
     6781 ccgattaaag ggcgatccta gcattaccat ctcttcagcc atgggaaatg ccatcattgc
     6841 tctgattatc tcgagtatgt tctttaacct caaggacgat acttcgagct ttttccagcg
     6901 cggctctctc ctcttctttg ccattgtcat caacgccttc agttccggtc tcgaaatgct
     6961 taccctctac gctcaaagac cgattgtcga aaagcactct cgatttgctc tctaccatcc
     7021 gtctgccgag gccatcgcgt ctatgctgat ggacctgccg tacaagaccc tcaacgctat
     7081 ctcatctaac ctaattctct acttcatgac gaacttgcgg agagagcctg gaaatttctt
     7141 cttcttcgtc tttacctcgt ttgtcctcac cctgaccatg tccatgtttt tccgatccat
     7201 cgcatccctg accaggtcgc tcgtcgaggc cctgccattt gccgcgattt tgatcacggg
     7261 tctcacaatg tacactggct ttaccattcc aacatcttac atgcccggct ggtctcgctg
     7321 gatggcctac attgatccca tcgcatacgg attcgagtcc atcatggtca acgagttctc
     7381 cggccgcgaa ttcctttgcg tcaactacgt tcctgcgggt cccagctaca atgttggtgg
     7441 caacaaccgt gtgtgttcca ccgtcggatc cgttcccgga cagccctttg tcctcggcga
     7501 cgactacatc aggtctacat acggctatga ggccagccgc aagtggagga acgtcggcat
     7561 catctttgct ttcatggtca ttctctgcgc catctacctg gttgcttcag actttatcac
     7621 cgagaagaag tccaagggtg aaatcctcgt cttccgccgt ggacacaaga acctcgacag
     7681 gagcactggc caggatgatg tcgaaggcgg cagcgagcgc accactactg cggcaaacac
     7741 caagtccgac gacattgcca ttattgagca acaaactgcc attttccagt ggaaggacat
     7801 ttgctacgat atccagatcc aaaaggaacg ccgtaggatt cttgatcacg ttgacggatg
     7861 ggtcaaacca ggtaccttga cggccctcat gggtgtctct ggagccggaa agactacgct
     7921 tttggatgtt ctcgctagtc gtaccaccat gggtgttatc tctggcgaaa tgctggttga
     7981 tggcaaggag cgagacgatt cgttccagcg caagactggt tatgcccaac aacaggatct
     8041 ccacttgagc actgctaccg ttcgcgaggc tcttaccttc tccgctcttc ttcgccagcc
     8101 cgctcacatt cctcgcgaag agaagattgc ctatgttact gaagtcatta agctccttga
     8161 gatgaccgag ttcgccgacg ctgttgttgg tatccccggc gagggcctca atgttgaaca
     8221 acgtaaacga ctgacaatcg gtgtcgagct tgccgctcgg ccagctctgc ttctcttcct
     8281 cgacgaaccc acttctggtc ttgattctca gacatcatgg gcgattctcg acctcctgga
     8341 caagctcaag aaaaacggtc aggccattct ttgcaccatc caccagccgt cggccatgct
     8401 gttccagcga ttcgatcgcc tgctcttcct caagtccggt ggccagactg tgtactacgg
     8461 cgacgttggc gagaactcca agatcctcat cgactacttc acgcgcaacg gcggacctcc
     8521 atgcccgcct gccgccaacc ccgcagaatg gatgctcgag gttatcggcg cagctcccgg
     8581 atcccacact gacattgact ggcacgacac ctggcgcaag tcgcccgaat acgcatacgt
     8641 tcaagcgcac ctcgctgagc tcaaggaaga gcgctctcgc atgacagacc taagccgcac
     8701 cgcctcccgg caggcacacg acgccgccag cttccgagag ttcgctgcgc ccttttgggc
     8761 gcaattctac gaggtgcaac tgcgcgtgtt ccagcagctc tggcgcacac cgacgtacat
     8821 ctactccaaa gccttcctct gcgtcagcac gtccctctac gttggcttct cgctctacaa
     8881 cacgcccaac acgctgcagg gcctgcaaaa ccaaatgttc gccatcttca ccctcttctt
     8941 cctctttggc cagttcatcc agcaaatcat gccgcacttt gtcgcccagc gcgcgctgta
     9001 cgaggcgcgc gaacggccct ccaagacgta ctcgtggaaa gccttcatca tgtccaacat
     9061 cattgtcgag ctcccctgga acacgctcat gtccgtcctg ctcttcctct gctggtacta
     9121 ccccatcggg ttgtcgcaca acgccgaggc caccgactcg acggcgctgc gcggcgccca
     9181 aatgtggctc ttcgtctggg tcttcctcat gttcgcctcg acctttgccc actttatgat
     9241 tgccgccctg gacacggccg agaacgcggg caacatgggc aacctgctct tcacgctctg
     9301 cgtcatcttc tgcggcatcc tcaccacccc cgagcagatg ccgcgcttct ggatcttcat
     9361 gtaccgcgtc tcgcccttta cctatctcgt cggcggcatg atggccgtcg gcgtcgccga
     9421 ctcgtccgtt acctgtgccg caaacgagta tctgcatttc gaccccgtca acggcacctg
     9481 cggcgagtac atggcacagt accaggccat ggccggtggc tacgtccaga accccagtgc
     9541 cacgtccaac tgcgctttct gccccatggg cgacaccaac gtcttcctca agacggtcca
     9601 tgcagagtac tcgaatgtct ggcgcaactt tggcctcatg tgggtctttg tcgttttcaa
     9661 cgcctttgcc gcttgtggct tgtactactg ggcccgtgtg cccaagcgcc cgacgattga
     9721 gaaggaggcg gccccgattc gcgcagaggg ttcgctagat agcgagacgg tggttggaag
     9781 gacttctgct gtcgagcagc aggatgctga gaagagagct gcattttagc cggggatctg
     9841 tagtagctcg tgaagggtgg agagtatatg atggtactgc tattcaatct ggcattggac
     9901 agtgagtttg agtttgatgt acagttggag tcgttactgc tgtcatcccc ttatactctt
     9961 cgattgtttt tcgaacccta acgccaagca cgctagtcta ttataggaaa ggatcccagg
    10021 aaacagctat gaccatgatt acgatatcat ggtgttttga tcattttaaa tttttatatg
    10081 gcgggtggtg ggcaactcgc ttgcgcgggc aactcgctta ccgattacgt tagggctgat
    10141 atttacgtaa aaatcgtcaa gggatgcaag accaaagtag taaaaccccg gagtcaacag
    10201 catccaagcc caagtccttc acggagaaac cccagcgtcc acatcacgag cgaaggacca
    10261 cctctaggca tcggacgcac catccaatta gaagcagcaa agcgaaacag cccaagaaaa
    10321 aggtcggccc gtcggccttt tctgcaacgc tgatcacggg cagcgatcca accaacaccc
    10381 tccagagtga ctaggggcgg aaatttaaag ggattaattt ccactcaacc acaaatcaca
    10441 gtcgtccccg gtattgtcct gcagaatgca atttaaactc ttctgcgaat cgcttggatt
    10501 ccccgcccct ggccgtagag cttaaagtat gtcccttgtc gatgcgatgt atcacaacat
    10561 ataaatacta gcaagggatg ccatgcttgg aggatagcaa ccgacaacat cacatcaagc
    10621 tctcccttct ctgaacaata aaccccacag caagctccga attcgaatcg atttgagcta
    10681 gatgtcttct atacagttgc aaaagtacct tgatctcctg cctgagaatg ccattctcaa
    10741 cgcaggtatc gccatcgcag gactcagcgc agcctatgct attggcttgg tgatctaccg
    10801 cctttacctc tctcccatct ccaagttccc cggtcccaag atcgctgcag caaccttctg
    10861 gtacgagctg tactacgatg tcatccacaa gggacagtac ttccacaaga tcgaggagat
    10921 gcacgaaaag tatggtacgt accacgacag ctttccccga taccccggat cctcgttgtg
    10981 caaacccctc tacccggtag accgtaaatt acacgaattc taacgtcaat gcttaggccc
    11041 gattgtaagg atcaaccccc acgagctgtc catccgcgat cccgactact acgatgagct
    11101 ctacgtctct ggctctgtcc gccccagcga ccgttacgag ggtttcgtca atggtgtcgt
    11161 cgacttcgag ggctctcact tggccactgt ggcccacgag cttcaccgca agaggagaaa
    11221 gcctctcgac ccttacttct cccgcgccgg tgtcaacagg ctggagccca tggtcgccga
    11281 tctcaccgag gagctcgttg tcaagcgttt tgaagagttc aagggcactg gcaaggtcgt
    11341 caggctcgac cacgccttca ctgcctactc tggcgatatc atcagcgctc tctgcatcga
    11401 cgaaccaccc cacttcacct ccacccctga cttcactccc agctggttcg atcttttcca
    11461 cagtggtgtc gttacactcc ctctcttcat gggtctccct tggcttatcc agtaaggaac
    11521 atcccacacc cgcaaagcct acatgtgcta attagtatag ccttatccgt cttatccccg
    11581 agtctatcct cgctgtcatc gaccctggtg cgcagaactg gaacaccttc aggatggtac
    11641 gttcagttcc tcaaccacaa gtccgaatat tgcagccata ctaactgttt ggcagatgtg
    11701 ctatgacagc atcaaggaca ccaagcgtga gaagggcgcc cagcccacca aggatacctc
    11761 gcttctcggc cgccctaccc tcttccgtca ccttgtcaac agcgatctcc ccgcctccga
    11821 gctttccgat gagcgtctcc tccgtgaggc tcaggtcctc atcggctccg gcaccatgac
    11881 caccgctggt accatgtgct tccttgtcta ctacatcaag tccaaccccg agatccaccg
    11941 ccgtctgacc gaagagctca agcccatcat ggagggatac ccccacaaga agcctagctg
    12001 ggctgagatc gaaaaggccg agtacctcca agccgttctc aaggaaggtc ttcggtacgt
    12061 ccctccccca caagatgatc cacacccaaa cacacacata gatgattgga aaactaacac
    12121 actacactaa tagtctgagc ttcggtacca ttcaccgccg cccacgtgtc tctcccaacc
    12181 agcccctgca gttcaaggag tgggtcatcc ccgctggcgt ccccgtcggc atgtccgcct
    12241 acttccagca cacggacccc aagatcttcc ccaaccccca cgagttcaac cccgacaggt
    12301 ggctcagcaa cgtcactcct gccatgaaga agaactacgt tcccttctcc aagggctccc
    12361 gccactgcct cggaatgaag taagtttttt cctctccctt ctttctctct cacatacaac
    12421 gaaactaacg agcattttcc agtcttgcct actgcgaact caactacatc atcgccacca
    12481 tgttccgtcc tggtgccgtc gacttcgacc tcttcgagac caccgagttg gatgtcaagc
    12541 ccacacacga catggttgtc cctctgccca gcctcaagtc caagggcttc aaggtcaagt
    12601 tcaactaggg gttgccttaa tctggtgtgg gatggacttt gaatcgtttc acaattaaat
    12661 tgtagatact tatagaatac gtggcgagaa aaactcaagt cgtcgcctct tctcgtctgt
    12721 aggcctgctg tcaagtatcc catcataggt tgtctcctca actgcacatc ctcaatccca
    12781 atacagtgca ggtcaaagaa gatttggaat aaattcttcg agcatcgaga tctagcttag
    12841 cccagttgtg aggttatgta tagcgtttcg tcttgatata tttgtaattc cacggtcaat
    12901 taattcattg agctgtcatt catctcgcga accgaaccga taccatgcca ctctgcatac
    12961 taacgggatg cttcgacctt tttcagttcc tccctgcagt caaaccaaca atattagtac
    13021 catgtctcga cgtaatatca acagccacaa tggcaccgaa cgcctagcaa ctgctggagc
    13081 tgtggccacc acggccacca taggggacta atacgcactt caactgcttc gagaagtcct
    13141 cagcggcatc atcgtcatcc cagctctctt cccaaaggtg accattagcg ccgtttgctg
    13201 atccagaagc ttgttcggtt tcttcttgtg gccagtctgt agatggaaac cgtcggtcag
    13261 cgcaactctc atctcacgaa gcaatcgaag cttccagtaa tgcaatcagg tcgctggtct
    13321 tccttgctat tttcaccggg cggaatccac tattattgtc tgaccttcaa cggggaaatc
    13381 ctcgaattca tcgtcttcct ccaacacggg gggcttctgc tgttggggtt gctgtgaagc
    13441 ttcgggctta tcgtgggatt gtgttgagtt cgacatggtg ggtgggggtg agagaacgat
    13501 gcgtttggag tattaggcga tagaccagat atgttttcaa aagtggatat tcaggaaggg
    13561 ctcgaatgtg agagagccga agctagggtg agccaccgcc gtgaggctgg ttgacatgaa
    13621 tagcccgcca atcaatccat ctagaatccc agctccgaag ctcaaggtac tgaccaaaac
    13681 caagatgaac gccacatacg gcagatcacc cgtccaccaa acgccgacaa agcagccgat
    13741 ggatacaaac accgagataa taaagtgagc caggcgccaa cgccgctgag accgttcgcg
    13801 ttgaacatag gtgtgaatca tgtcgatgat acgatcatag gtgtctggag atagggctgt
    13861 attcggaacc acggactggc gaagtttatt gtctgtcaag ttgtccgctt cagagggacg
    13921 cggagagcgg gtgatggtgg cttcgacggc aaaatggtcg ctcagcgagc aacggagcgt
    13981 gggatgacgc tgtgtcatgc cgatattgac ggactcgacg gaccattggg gcgcagggaa
    14041 ggcaggcgga tatccgccgt ctccgacaaa gatataatcc agccgttttc ccctcggaca
    14101 gggagcatcc ttgtcgacag cgatctcctc ccccttctct agccgtttgc gttcctcctt
    14161 ggtccagcgc caggtgttga atttgccgtc gcatgtggcg ccgttcacgt ggaggttgaa
    14221 ctcggctgac ggggtcggtt tgcctcgttt ctgttcaatg gggtcgatgg gggcggccac
    14281 ggacgagtct gggtatatag cctgccatac atcctggacg ggactatggg cgcggataag
    14341 ctggtgtgcg aacgacgacg gcaccatatt aaagtcgccc agtccaatgg caaggtggcc
    14401 tctctctgcg gcgccgcgca tcaactttgc gatttcccaa gcttgcgcgg tgcgatggca
    14461 gatatacgaa tcattcggtt ccttctcgta aggggcgtgg agatgcgtgc agaacacttc
    14521 ggcgacgtcg ctggctccgg gaccgaagcg tacacgggca caggcaacac ccttcccgac
    14581 gtaccagtcg ccgcggaaga ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg
    14641 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    14701 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    14761 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    14821 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    14881 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    14941 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    15001 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    15061 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    15121 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    15181 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    15241 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    15301 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    15361 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    15421 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    15481 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    15541 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    15601 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    15661 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    15721 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    15781 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    15841 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    15901 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    15961 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    16021 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    16081 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    16141 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    16201 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    16261 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    16321 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    16381 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    16441 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    16501 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    16561 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    16621 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    16681 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    16741 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    16801 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    16861 ggccctttcg tc
//
