LOCUS       sequence-460015-        9027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP localized to mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Bagnoli E, Lin YE, Burel S, Jaimon E, Antico O, Themistokleous C, 
            Nikoloff JM, Squires S, Morella I, Watzlawik JO, Fiesel FC, Springer
            W, Tonelli F, Lis P, Brooks SP, Dunnett SB, Brambilla R, Alessi DR, 
            Pfeffer SR, Muqit MMK
  TITLE     Endogenous LRRK2 and PINK1 function in a convergent neuroprotective 
            ciliogenesis pathway in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Feb 4;122(5):e2412029122. doi: 
            10.1073/pnas.2412029122. Epub 2025 Jan 28.
  PUBMED    39874296
REFERENCE   2  (bases 1 to 9027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-02-4"; volume: "122(5):e2412029122. 
            doi"; pages: " 10.1073/pnas.2412029122. Epub 2025 Jan 28"
COMMENT     Sequence Label: pMCB306-GFP-mito
FEATURES             Location/Qualifiers
     source          1..9027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             651..831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1340..1356
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1341..1357
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1360..1948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1466..1477
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1875..1895
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    2004..2037
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(2189..2899,2900..2902,2903..2905))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2231..2252)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2580..2599
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2839..2860
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2902..2911
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2918..2971)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2981..3577)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3061..3081)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3558..3577
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     intron          3598..4536
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3621..3641)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(4537..4748)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(4993..5013)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    5017..5050
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5303..5420
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5718..5762)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5947..6180
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6677..6802
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6849..7029
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7047..7245)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7072..7092)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7247..7626
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7798..7817
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7892..7996
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7997..8065,8066..8857)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8215..8234)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg
     1381 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     1441 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     1501 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     1561 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     1621 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     1681 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     1741 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     1801 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     1861 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     1921 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
     1981 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
     2041 gtccctcgac gaattctcaa gaccgtggca ggagcttgta tttgtacagc acaaacccca
     2101 gggcagttac tgatgtggaa aatccaatga tcttcagcag gccagaaaca gagggcaggt
     2161 tcctttccca gaaggcggcc gcacctccct tgtacagctc gtccatgccg agagtgatcc
     2221 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     2281 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     2341 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     2401 ggatcttgaa gttcgccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     2461 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     2521 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     2581 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     2641 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     2701 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     2761 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccagacacgc
     2821 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     2881 acagctcctc gcccttgctc accatggtgg cgaccggtgg gccaggattc tcctcgacgt
     2941 caccgcatgt tagcagactt cctctgccct ctcccgatcc ggcaccgggc ttgcgggtca
     3001 tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc
     3061 gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc
     3121 gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt
     3181 cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca
     3241 ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg
     3301 ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca
     3361 ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga
     3421 agagttcttg cagctcggtg acccgctcga tgtggcggtc cgggtcgacg gtgtggcgcg
     3481 tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggcccggggg acgtcgtcgc
     3541 gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt gggctagctc acgacacctg
     3601 aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa
     3661 ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc taacttcagt
     3721 ctccacccac tcagtgtggg gaaactccat cgcataaaac ccctcccccc aacctaaaga
     3781 cgacgtactc caaaagcgcg agaactaatc gaggtgcctg gacggcgccc ggtactccgt
     3841 ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc
     3901 acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag cagggccggg
     3961 aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg ccgcccaggg
     4021 cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag cttgagacta
     4081 cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg
     4141 ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag tgtgcagatc
     4201 ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag gtcatcaaaa
     4261 attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt gccaccagat
     4321 tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag gcgaaggggc
     4381 tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct cgggatcaag
     4441 aatcacgtac tgcagccagg tggaagtaat tcaaggcacg caagggccat aacccgtaaa
     4501 gaggccaggc ccgcgggaac cacacacggc acttacctgt gttctggcgg caaacccgtt
     4561 gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg
     4621 aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta
     4681 ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg
     4741 ctctgcccac tgacgggcac atgcatggcg gtaatacggt tatccacgcg gccgcctaat
     4801 ggatcctagt actcgagaaa aaaagcaccg actcggtgcc actttttcaa gttgataacg
     4861 gactagcctt atttaaactt gctatgctgt ttccagctta gctcttaaac gggtggtgcc
     4921 catcctggtc caacaaggtg gttctccaag ggatacttat agtctcaaaa cacacaatta
     4981 ctttacagtt agggtgagtt tccttttgtg ctgtttataa cttcgtataa tgtatgctat
     5041 acgaagttat agaaatccaa gcctatcatg taaaatgtag ctagtattaa aaagaacaga
     5101 ttatctgtct tttatcgcac attaagcctc tatagttact aggaaatatt atatgcaaat
     5161 taaccggggc aggggagtag ccgagcttct cccacaagtc cgtgcgaggg ggccggcgcg
     5221 ggcctagaga tggcgcgtcg gatctctaga gcgggcccgg tactaaccaa actggatctc
     5281 tgctgtccct gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt
     5341 tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa
     5401 tccccccttt tcttttaaaa ttgtggatga atactgccat ttgtctgcag aattggcgca
     5461 cgcagtgccg atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct
     5521 tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat
     5581 aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt
     5641 aaacctacca agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt
     5701 atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc
     5761 ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta
     5821 atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta
     5881 ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa
     5941 atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc
     6001 tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc
     6061 aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc
     6121 attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct
     6181 attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact
     6241 cctaatggtt caatttttac tactttatat ttatataatt cacttctcca attgtccctc
     6301 atatctcctc ctccaggtct gaagatcagc ggccggccgc ttgctgtgcg gtggtcttac
     6361 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
     6421 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
     6481 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
     6541 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
     6601 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
     6661 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
     6721 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
     6781 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
     6841 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     6901 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     6961 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     7021 gagagaccca gtacaggcaa aacgcgctgc ttatatagac ctcccaccgt acacgcctac
     7081 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     7141 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     7201 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     7261 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     7321 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     7381 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     7441 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     7501 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     7561 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
     7621 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
     7681 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
     7741 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
     7801 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
     7861 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7921 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7981 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     8041 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     8101 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     8161 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     8221 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8281 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8341 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8401 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     8461 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8521 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8581 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8641 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8701 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8761 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8821 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8881 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8941 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     9001 ccccgtagaa aagatcaaag gatcttc
//
