LOCUS       sequence-460046-       10879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMVP expression vector for human SEC24C (G220C mutation, closed, 
            resistant to sgSEC24C #1 and #2) (Blasticidin selection marker).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Schiantarelli J, Benamar M, Park J, Sax HE, Oliveira G, Bosma-Moody 
            A, Campbell KM, Liu D, Johnson DB, Rodig S, Wu CJ, Hodi FS, Ribas A,
            Van Allen E, Haq R
  TITLE     Genomic mediators of acquired resistance to immunotherapy in 
            metastatic melanoma.
  JOURNAL   Cancer Cell. 2025 Feb 10;43(2):308-316.e6. doi: 
            10.1016/j.ccell.2025.01.009.
  PUBMED    39933900
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2025.01"; journalName: "Cancer Cell"; date: 
            "2025-02-10- 10"; volume: "43"; issue: "2"; pages: "308-316.e6"
COMMENT     Sequence Label: pMVP-SEC24C-G220C
FEATURES             Location/Qualifiers
     source          1..10879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1981..2005
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        2161..2540
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2541..2744
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2695..2715
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(2773..2793)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6086..6106
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6118..6159
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6187..6204)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6193..6400
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(6409..6433)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6485..6801
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6652..6787
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6849..6896
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6915..7313
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7400..7633
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7705..7826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7742..7761)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7796..7815
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7866..8001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8021..8040)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8022..8040)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8050..8067)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8050..8066)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8059..8081)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8208..8663
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8295..8314)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8505..8526
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8689..8793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8794..8862,8863..9654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9012..9031)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9825..10413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10314..10333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10567..10584
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10701..10722
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10737..10742,10743..10760,10761..10767)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10775..10791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10780..10802
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10799..10815
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10799..10815
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10834..10854
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10836..10854
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgtctaga ggatccacta gtccagtgtg gtggaattct gcagatatca
     1981 acaagtttgt acaaaaaagc aggcttacga tctatacatt gaatcaatat tggcaattag
     2041 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     2101 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
     2161 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     2221 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     2281 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     2341 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     2401 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     2461 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
     2521 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
     2581 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2641 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
     2701 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     2761 agatccggca cccaactttt ctatacaaag ttggccaaca tgaacgtcaa ccagtcagtt
     2821 ccacctgtgc caccatttgg gcagccccag cccatctacc cagggtatca tcagtccagc
     2881 tatggtgggc aatcagggtc cacagccccc gccattccct atggagccta caatggccca
     2941 gtaccaggct atcagcaaac acctccccaa ggtatgtcaa gagccccacc ttcctcggga
     3001 gcacctccag cctcaacagc acaggctcct tgtggccagg ctgcatatgg ccagtttggc
     3061 caaggagatg tacagaatgg gccaagctcc actgttcaga tgcaaaggct gcctgggtct
     3121 cagccatttg ggtccccatt ggcacctgtg ggcaaccagc cacctgtgct tcagccctat
     3181 ggccctcccc cgacaagtgc acaggtggct acgcagctgt ctggaatgca gatcagcggt
     3241 gctgtggccc cagcccctcc ttcttcaggg ctgggctttg gcccaccaac atcgctggct
     3301 tcagcctcag gaagtttccc taactctggt ctgtatggct cctatcctca gggccaggct
     3361 cctcccctta gccaggccca aggtcatcct gggatccaga ctccccagcg atctgcccca
     3421 tcacaggcct ccagcttcac acccccagct tcagggtgtc ctcggctgcc ttcgatgact
     3481 ggtccactcc tgcctggaca gagttttgga gggccctcag tgagccagcc caaccatgtg
     3541 tcttcacctc ctcaagctct gccccctggc acccagatga ctgggcccct gggaccactg
     3601 ccacctatgc actccccgca gcagccaggc tatcagcccc aacaaaatgg ttccttcgga
     3661 ccagcccggg gccctcagtc taattatgga ggcccctacc cagcagcacc cacctttggc
     3721 agtcagcctg ggcctcctca gccactgcct cctaagcgcc tggaccctga tgccatccca
     3781 agccctattc aggtcattga agatgacagg aacaaccggg gtacagagcc atttgttact
     3841 ggagtacggg gccaggtgcc acccttagtc actaccaact tcctggtgaa agaccaaggg
     3901 aatgcaagtc cccgatacat ccgatgtaca tcctataata tcccttgcac atctgacatg
     3961 gctaagcagg ctcaggtgcc cctggcagca gtcatcaaac cgctggcaag gctgccccca
     4021 gaggaggctt caccgtatgt tgtggaccat ggggaatctg gccctttgcg ctgcaaccgc
     4081 tgcaaagcat acatgtgtcc cttcatgcag ttcattgaag gagggaggcg tttccagtgc
     4141 tgtttttgca gctgtatcaa tgatgttccc ccccagtatt ttcagcacct ggatcatacc
     4201 ggcaaacgtg tggatgctta tgaccgccct gagctatccc tgggctctta tgaattcttg
     4261 gccactgtag attactgcaa gaacaataag ttccccagcc ctcctgcctt tatcttcatg
     4321 attgacgtct cctacaatgc catcaggact ggtcttgtta ggctcctctg tgaggagctc
     4381 aagtcactgt tagactttct acctagggag ggtggggcag aagagtcagc aatccgcgtt
     4441 ggctttgtca cctacaataa ggtgctccac ttctataatg tgaagagctc attggcccag
     4501 ccacagatga tggttgtgtc tgatgtggct gacatgtttg tgccactgct ggatggcttc
     4561 ctggtcaacg tcaatgagtc tcgggcagtt atcaccagct tattggatca gattccagaa
     4621 atgtttgcag acacaaggga aacagagaca gtatttgtac cagttatcca ggctggaatg
     4681 gaggctctga aggctgctga gtgtgcaggg aagctctttc tattccatac atccctgccc
     4741 attgcagagg ccccagggaa actgaagaac agagatgaca ggaagctgat caatacagac
     4801 aaggagaaga ctctgttcca gcctcagaca ggtgcctatc agaccctggc caaagagtgt
     4861 gtggcccaag gctgctgtgt agatctcttt ctcttcccta accagtatgt ggatgtggcc
     4921 acactctctg ttgtgcccca gctcactggt ggctctgtct acaaatatgc ttcctttcag
     4981 gtggagaacg accaggagcg gttcctgagt gacctgcgtc gtgatgtcca gaaggttgtt
     5041 ggctttgatg ctgtgatgcg ggtccggaca agcactggta tccgtgctgt agatttcttt
     5101 ggagctttct acatgagcaa cacgacagat gtggagctgg ctgggctaga tggggacaaa
     5161 acagtgactg tggagttcaa gcatgacgat cggctcaatg aagagagcgg agctctcctg
     5221 cagtgtgccc tgctttacac cagctgtgca gggcagcgtc ggctccgcat ccataatctg
     5281 gccctgaact gctgcaccca gctggctgat ctatatcgaa actgtgagac tgacacgctc
     5341 atcaactaca tggccaagtt tgcatatcgg ggagtcctga atagccctgt gaaggctgtt
     5401 cgtgacacgc tcatcaccca gtgtgcccag atcctggcct gttacagaaa gaactgtgct
     5461 agcccctcct ctgcaggaca gttgatcctt cctgagtgca tgaagctact cccagtttac
     5521 ctgaactgtg tgttgaagag tgatgtcctg cagcctggag ctgaagtcac tactgatgac
     5581 cgtgcctatg tccgacagct agttacctcc atggatgtga ctgagaccaa tgtcttcttc
     5641 taccctcggc tcttaccttt gacaaagtct cccgttgaga gtactaccga accaccagca
     5701 gttcgagcct ctgaagagcg tctaagcaat ggggatatat atttactgga gaatgggctc
     5761 aacctcttcc tctgggtggg agcaagcgtc caacagggtg ttgtccagag ccttttcagc
     5821 gtctcctcct tcagtcagat caccagtggt ttgagtgttc tgccagttct ggataatcca
     5881 ctgtccaaga aggttcgagg cctcattgat agcttacggg cacagagatc ccggtacatg
     5941 aagcttaccg tggtgaaaca ggaagacaag atggagatgc tgttcaagca cttcctggtg
     6001 gaagacaaga gtctgagtgg gggagcatct tatgtggact ttctctgtca tatgcacaag
     6061 gagattcggc agctactgag ctaaacaact ttgtataata aagttggtgg cggatccggt
     6121 aagcctatcc ctaaccctct cctcggtctc gattctacgt aagaattcct agagctcgct
     6181 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     6241 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     6301 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     6361 agggggagga ttgggaagag aatagcaggc atgctgggga ggtacctgac ccagctttct
     6421 tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag gtggcgatgg
     6481 ttccggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6541 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6601 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6661 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6721 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6781 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     6841 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     6901 tgaggaacta aaccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     6961 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     7021 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     7081 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     7141 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     7201 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     7261 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagcacaat
     7321 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7381 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     7441 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7501 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7561 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7621 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7681 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     7741 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7801 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     7861 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7921 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7981 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     8041 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8101 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8161 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     8221 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8281 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8341 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8401 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8461 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8521 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8581 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     8641 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     8701 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8761 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8821 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8881 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8941 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9001 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9061 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9121 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9181 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9241 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9301 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9361 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9421 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9481 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9541 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9601 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9661 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9721 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9781 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9841 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9901 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9961 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10021 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10081 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10141 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10201 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10261 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10321 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10381 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10441 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10501 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10561 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10621 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    10681 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    10741 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    10801 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
    10861 aagctggagc tgcaagctt
//
