LOCUS       sequence-460086-        1962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-5xA which 
            contains only 5 TRAF6 binding motifs with a alanine mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-5xA
FEATURES             Location/Qualifiers
     source          1..1962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(907..909,910..1620)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(949..970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1210..1229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1557..1578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgtctgcgg gagacccccg cgtgggatcc gggtccctgg actccttcat gttctccata
       61 cccttggtcg cgcttaacgt gggagtgagg cgccgcctat cgctgttctt gaaccctcgg
      121 acgcccgtgg cggccgactg gaccttgctg gcggaggaga tgggcttcga gtacttggag
      181 atccgagagc tggaaacgcg ccctgacccc actcgcagtt tgttggatgc ctggcagggg
      241 cgctctggcg cgtctgtcgg caggctgcta gagctgctgg ccttgttaga ccgtgaggat
      301 atactgaagg agctgaagtc gcgcatcgag gaggactgcc agaaatactt aggtaagcag
      361 cagaaccagg agtccgagaa gcctttacag gtggccagag tggaaagcag tgtcccacaa
      421 acaaaggaac tgggaggcat caccaccctt gatgaccccc taggacaaac gccggaactt
      481 ttcgatgcct ttatctgcta ctgccccaac gatatcgagt ttgtgcagga gatgatccgg
      541 caactagaac agacagacta tcggcttaag ttgtgtgtgt ccgaccgtga cgtcctgccg
      601 ggcacctgtg tctggtccat tgccagcgag ctaattgaga aaaggtgtcg ccgcatggtg
      661 gtggttgttt ctgacgatta tctacagagc aaggaatgtg acttccagac caagtttgca
      721 ctcagcctgt ctccaggtgt ccaacagaag cgactgattc ctattaaata caaggcgatg
      781 aagaaggact ttcccagtat cctgcggttc atcactatat gcgactatac caacccttgc
      841 accaagtcct ggttctggac ccgccttgcc aaggctttgt ccctgcccgg aggtagtggc
      901 gggtccgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      961 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1021 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1081 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     1141 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1201 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1261 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1321 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     1381 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1441 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1501 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     1561 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1621 ggtggaagtc ctaaacaaga acctcaagcg atcgatttcg gaggaggcag tggcggcaca
     1681 ggatccggcg ggggcagcgg tgggcccaaa caggaaccac aggcaattga tttcggcggc
     1741 gggtctggag gtacaggtag cgggggcact ggaggctctc caaagcagga gcctcaggcg
     1801 atagactttg gcggggggag cggcggtact ggatctggcg gtggcagtgg tggccctaag
     1861 caggagcccc aagcaatcga ctttggggga ggcagtggtg ggaccggatc aggaggaact
     1921 gggggtagtc ccaagcaaga accacaagcg atcgactttt aa
//
