LOCUS       sequence-460102-        1932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-17xTA with 
            alanine mutation in the TRAF6 binding motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-17xTA-3xAla
FEATURES             Location/Qualifiers
     source          1..1932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1216..1218,1219..1932)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1258..1279)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1519..1538)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1866..1887
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgacagcca ctgcaaccgc aacagctaca gctaccgcca cagcaaccgc aacagcaacc
       61 gcaactgcta ctgcaacagc aacagcaacc gccacagcaa cagccggagg atcaggcgga
      121 tcatccgaaa agcctttaca ggtggccaga gtggaaagca gtgtcccaca aacaaaggaa
      181 ctgggaggca tcaccaccct tgatgacccc ctaggacaaa cgccggaact tttcgatgcc
      241 tttatctgct actgccccaa cgatatcgag tttgtgcagg agatgatccg gcaactagaa
      301 cagacagact atcggcttaa gttgtgtgtg tccgaccgtg acgtcctgcc gggcacctgt
      361 gtctggtcca ttgccagcga gctaattgag aaaaggtgtc gccgcatggt ggtggttgtt
      421 tctgacgatt atctacagag caaggaatgt gacttccaga ccaagtttgc actcagcctg
      481 tctccaggtg tccaacagaa gcgactgatt cctattaaat acaaggcgat gaagaaggac
      541 tttcccagta tcctgcggtt catcactata tgcgactata ccaacccttg caccaagtcc
      601 tggttctgga cccgccttgc caaggctttg tccctgcccg gaggtagtgg cgggtcccct
      661 ccttctcccc aagccaactc ctatgtcagc tccactggta gggctcactc cggagccgca
      721 ccatggcagc cactcgctgc accatcaggt gcttcagccc aggccgcaga gcaactgcaa
      781 cggggaccca accagccagt cgcgtccgac gaaagtcttg gagggctgag cgctgctctg
      841 cggtcctggc accttacacc aagttgtccc cttgatcctg ctcctctgcg ggaggctggg
      901 tgcccccaag gtgacacagc aggggaatcc tcatggggtt caggacctgg gagccggcca
      961 accgctgttg aaggcctggc tttgggctcc tctgcatcat catcatcaga acctccccag
     1021 attatcatca atccagctcg ccagaaaatg gttcagaaat tggctttgta tgaggatggt
     1081 gccctggatt ccttgcaatt gctgtcatct tcttctcttc ccggacttgg acttgaacaa
     1141 gacagacaag gtccagaggc gtccgacgag ttccagagcg gaggttccgg gggtactggg
     1201 ggtagtggag gaagtgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1261 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     1321 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1381 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     1441 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1501 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1561 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1621 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1681 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1741 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1801 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1861 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1921 ctgtacaagt aa
//
