LOCUS       sequence-460106-        1884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-TIR with 5 
            TRAF6 binding motifs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-TIR-5xT6BMs
FEATURES             Location/Qualifiers
     source          1..1884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1168..1170,1171..1884)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1210..1231)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1471..1490)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1818..1839
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgccacaaa caaaggaact gggaggcatc accacccttg atgaccccct aggacaaacg
       61 ccggaacttt tcgatgcctt tatctgctac tgccccaacg atatcgagtt tgtgcaggag
      121 atgatccggc aactagaaca gacagactat cggcttaagt tgtgtgtgtc cgaccgtgac
      181 gtcctgccgg gcacctgtgt ctggtccatt gccagcgagc taattgagaa aaggtgtcgc
      241 cgcatggtgg tggttgtttc tgacgattat ctacagagca aggaatgtga cttccagacc
      301 aagtttgcac tcagcctgtc tccaggtgtc caacagaagc gactgattcc tattaaatac
      361 aaggcgatga agaaggactt tcccagtatc ctgcggttca tcactatatg cgactatacc
      421 aacccttgca ccaagtcctg gttctggacc cgccttgcca aggctttgtc cctgcccgga
      481 ggtagtggcg ggtcccctcc ttctccccaa gaaaactcct atgtcagctc cactggtagg
      541 gctcactccg gagccgcacc atggcagcca ctcgctgcac catcaggtgc ttcagcccag
      601 gccgcagagc aactgcaacg gggacccaac cagccagtcg agtccgacga aagtcttgga
      661 gggctgagcg ctgctctgcg gtcctggcac cttacaccaa gttgtcccct tgatcctgct
      721 cctctgcggg aggctgggtg cccccaaggt gacacagcag gggaatcctc atggggttca
      781 ggacctggga gccggccaac cgctgttgaa ggcctggctt tgggctcctc tgcatcatca
      841 tcatcagaac ctccccagat tatcatcaat ccagctcgcc agaaaatggt tcagaaattg
      901 gctttgtatg aggatggtgc cctggattcc ttgcaattgc tgtcatcttc ttctcttccc
      961 ggacttggac ttgaacaaga cagacaaggt ccagaggagt ccgacgagtt ccagggtggc
     1021 agtcctaagc aggaacctca agaaatcgac ttcggtgggg gctcaggtgg aactgggtca
     1081 ggtggcggtt ctggaggtcc aaaacaagaa ccacaggaga tagactttag cggaggttcc
     1141 gggggtactg ggggtagtgg aggaagtgtg agcaagggcg aggagctgtt caccggggtg
     1201 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1261 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1321 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     1381 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1441 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1501 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1561 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1621 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1681 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1741 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
     1801 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1861 ggcatggacg agctgtacaa gtaa
//
