LOCUS       sequence-460512-        4877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            mScarletI3 with Kan selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4877)
  TITLE     Teusink Lab yeast expression plasmids
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-ymScarletI3-Kan
FEATURES             Location/Qualifiers
     source          1..4877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     125..143
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(181..203)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     303..322
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        403..421
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     403..420
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1211..1398
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1266..1290)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            1473..2829
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1473..1816
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1817..2626
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1884..1903)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2632..2829
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2933..2952)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2934..2952)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3039..3056)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3210..3798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3290..3309)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3969..4760,4761..4829))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4592..4611
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(4830..4877,1..57))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
       61 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
      121 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
      181 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
      241 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      301 tgcggcatca gagcagattg tactgagagt gcaccatatg gacatattgt cgttagaacg
      361 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
      421 aacgcggccg ccagctgaag cttcgtacgc tgcaggtcga cggatcggtg acggtgctgg
      481 tttaattaac atggattcta ctgaagctgt tattaaggaa ttcatgagat ttaaggttca
      541 tatggaaggt tctatgaatg gtcatgaatt tgaaattgaa ggtgaaggtg aaggtagacc
      601 atatgaaggt actcaaactg ctaagttgaa ggtcactaag ggtggtccat tgccattctc
      661 ttgggatatt ttgtctccac aattcatgta cggttctaga gcttttatta agcatccagc
      721 tgacattcca gattactgga agcaatcttt cccagaaggt tttaaatggg aaagagttat
      781 gatctttgaa gatggtggta ctgtttctgt tactcaagat acttctttgg aagatggtac
      841 tttgatttac aaggttaagt tgagaggtgg taacttccca ccagatggtc cagttatgca
      901 aaagagaact atgggttggg aagcctctac tgaaagattg tacccagaag atgttgtttt
      961 aaagggtgat attaagatgg ctttgagatt gaaggatggt ggtagatact tggctgattt
     1021 taagactact tataaggcta agaagccagt tcaaatgcca ggtgctttca acattgatag
     1081 aaagttggat attacttctc ataacgaaga ttacactgtt gttgaacaat acgaaagatc
     1141 tgttgctaga cattctactg gtggttctgg tggttcttaa gcggccgcct cgaggtccac
     1201 ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     1261 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
     1321 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg tcgctcttat
     1381 tgaccacacc tctaccggca gatccgctag ggataacagg gtaatataga tctgtttagc
     1441 ttgcctcgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat accctccttg
     1501 acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac atttagccca
     1561 tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg gcgcgaagca
     1621 aaaattacgg ctcctcgctg cagacctgcg agcagggaaa cgctcccctc acagacgcgt
     1681 tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat ttgccactga
     1741 ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct cacatcacat
     1801 ccgaacataa acaaccatgg gtaaggaaaa gactcacgtt tcgaggccgc gattaaattc
     1861 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     1921 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     1981 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2041 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     2101 caccactgcg atccccggca aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     2161 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     2221 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2281 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     2341 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg
     2401 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     2461 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     2521 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     2581 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag tactgacaat
     2641 aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat tgtagttgtt
     2701 ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat catctgccca
     2761 gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc
     2821 gctatactgc tgtcgattcg atactaacgc cgccatccag tttaaacgag ctcgaattca
     2881 tcgatgatat cagatccact agtggcctat gcggccgcgg atctgccggt ctccctatag
     2941 tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg ccaacgcgcg
     3001 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     3061 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3121 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3181 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3241 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3301 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3361 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3421 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3481 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3541 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3601 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     3661 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3721 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3781 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3841 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3901 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3961 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4021 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4081 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4141 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4201 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4261 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4321 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4381 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4441 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4501 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4561 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4621 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     4681 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4741 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4801 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4861 tatcagggtt attgtct
//
