LOCUS       sequence-46061-e        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Saini DK, Kalyanaraman V, Chisari M, Gautam N
  TITLE     A family of G protein betagamma subunits translocate reversibly from
            the plasma membrane to endomembranes on receptor activation.
  JOURNAL   J Biol Chem. 2007 Aug 17;282(33):24099-108. Epub 2007 Jun 20.
  PUBMED    17581822
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-17"; volume: "282(33)"; pages: "24099-108. 
            Epub 2007 Jun 20"
COMMENT     Sequence Label: YFP Gamma 2
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(269..982)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(269..982)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(309..329)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     924..952
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattcttaa aggatagcac
       61 agaaaaactt cttctcccta aatgggtttt ctgaagccgg aacaggtgtc aggaggggat
      121 cttccttggc atgcgcttca cagtaggcca tcaaatctgc agctgccttg gacaccttta
      181 tcctatcgat gttggcttcc atcttcagct gttctaccag tttcctggct tgtgctatgc
      241 tggcggtgtt gttgctggcc atactagttt tgtatagttc atccatgcca tgtgtaatcc
      301 cagcagctgt tacaaactca agaaggacca tgtggtctct cttttcgttg ggatctttcg
      361 aaagcttaga ttgataggac aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa
      421 ttggagtatt ttgttgataa tggtctgcta gttgaacgct tccatcttca atgttgtgtc
      481 taattttgaa gttaactttg attccattct tttgtttgtc tgccatgatg tatacattgt
      541 gtgagttata gttgtactcc aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa
      601 taccttttaa ctcgattcta ttaacaaggg tatcaccttc aaacttgact tcagcacgtg
      661 tcttgtagtt cccgtcatct ttgaaaaata tagttctttc ctgtacataa ccttcgggca
      721 tggcactctt gaaaaagtca tgctgtttca tatgatctgg gtatcttgca aagcacataa
      781 gaccataacc gaaagtagtg acaagtgttg gccatggaac aggtagtttt ccagtagtgc
      841 aaataaattt aagggtaagt tttccgtatg ttgcatcacc ttcaccctct ccactgacag
      901 aaaatttgtg cccattaaca tcaccatcta attcaacaag aattgggaca actccagtga
      961 aaagttcttc tcctttactc atggntccga nctcggtacc aagcttaagt ttaaacgcta
     1021 gccagct
//
