LOCUS       sequence-460674-       12511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A gateway compatible destination vector containing the murine 
            beta-MHC promoter for embryonic and postnatal cardiomyocyte 
            expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 12511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pBeta-MHC-promoter DV (JDW 26)
FEATURES             Location/Qualifiers
     source          1..12511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     721..737
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    752..1374
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     1156..1175
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     protein_bind    1395..1519
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1560..1865)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1662..1681)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2207..2866)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2781..2800
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2920..2926,2927..2944,2945..2950))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2975..3099)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(10270..10286)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(10323..10341)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10362..10378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10362..10378)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10375..10397)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10386..10402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10410..10416,10417..10434,10435..10440))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10455..10476
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10593..10610)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10764..11352)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10844..10863)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11523..12314,12315..12383))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12146..12165
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12384..12488)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgatcg
      661 atcgctccac cgcggtggcg gccgcccggg cgccacgtgt atactagttt aaacggccgc
      721 tctagaacta gtggatcccc cgggctgcag gaattcaaca ggcatctact gagtggaccc
      781 aacgcatgag aggacagtgc caagcaagca actcaaatgt cccaccggtt gggcatggcc
      841 aggtagccta tgctgtgtct ggacgtcctc ctgctggtat agttatttta aaatcagaag
      901 gacagggaag ggagcagtgg ttcacgcctg taatcccagc aatttgggag gccaaggtgg
      961 gtagatcacc tgagattagg agttggagac cagcctggcc aatatggtga aaccccgtct
     1021 ctaccaaaaa aacaaaaatt agctgagcct ggtcatgcat gcctggaatc ccaacaactc
     1081 gggaggctga ggcaggagaa tcgcttgaac ccaggaggcg gagattgcag tgagccaaga
     1141 ttgtgccact gcactccagc ttggttccca atagaccccg caggccctac aggttgtctt
     1201 cccaacttgc cccttgctcc ataccacccc cctccacccc ataatattat agaaggacac
     1261 ctagtcagac aaaatgatgc aacttaattt tattaggaca aggctggtgg gcactggagt
     1321 ggcaacttcc agggccagga gaggcactgg ggaggggtca cagggatgcc acccatcaag
     1381 ctcgacgcgt tatcaccact ttgtacaaga aagctgaacg agaaacgtaa aatgatataa
     1441 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
     1501 aacatatcca gtcactatgg tcgacctgca gactggctgt gtataaggga gcctgacatt
     1561 tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga
     1621 tcagccactt cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg
     1681 cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac
     1741 agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca
     1801 ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac
     1861 tgcatttcac cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc
     1921 gacctcagcc atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc
     1981 ttcattctgc atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc
     2041 aactgatagc tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt
     2101 ttgacatact tcgggtatac atatcagtat atattcttat accgcaaaaa tcagcgcgca
     2161 aatacgcata ctgttatctg gcttttagta agccggatcc acgcgtttac gccccgccct
     2221 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     2281 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     2341 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     2401 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     2461 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     2521 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     2581 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     2641 ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     2701 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     2761 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     2821 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     2881 ctcctgaaaa tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat
     2941 aaagtgtaaa gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt
     3001 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     3061 attttacgtt tctcgttcag cttttttgta caaacttgtg atactggctg agcctggggt
     3121 gaggagcagc tgttacctat atgccttccc gccccttggg ctctcccact cagtggcagc
     3181 tcccaaatcc ggtactgtgc tcagcactca ggagtttacc cttctccagg ctcaccccta
     3241 aaccagagag cagcctggca actgtgccaa ggcaaggggg cagctttact ttcattcact
     3301 tggggaactc tttgggtgtg tcctgaccgt ctttcgggga cagaactctg tatcaatctc
     3361 tgactgtcaa tagtccctgc ccctcactgg ccaggccatt gtctctgagc aggcagaaag
     3421 gaacctgcca ggcttcgggc ttggggcttt gggctttaac tatgtgcagt tttgccagat
     3481 ctcccaacat ttggactcca acttttctca ccttggattc tcaaacgtgt ctagtgatag
     3541 agaggtaagc ccaggcctgt aggagagctg gagaaaggga agagacggtg tcaggacagg
     3601 ctgtggccca gcctggcttc tggcacctcc ctcctctctg gctctgttct tcagcacgcg
     3661 gaagagtgat tagcagagct ggtcagcctt tctggtcact ggcatccttc cttttcaagc
     3721 tctatctcct ttctcccaaa gactaacaac ctccgagccc cagtcctccg cccttagggg
     3781 ctgttctacc cttacctgta ctcctctgct gaggcttcct ttctcggagc cacctacaac
     3841 acaacaatag tcacaatgca ctcttgctgt tcactctcac ggctgcccct tgggtacgtg
     3901 aggaagtgga ggctgaagcc ttacttttct cagagcacag gacaagtagc agagtccaga
     3961 tttcagactc gagcctcatg attccaacag ccctctgact accctcgcac accccagcct
     4021 cccctgcaca cgccctgata aatacacatg ccttctgctt gtgcccacac ttgacgtgaa
     4081 tgtaaacttc cttccatcct cagcacacat gctccccaca gtgttcatgt gcaaacagcc
     4141 cagcacatag gcaggctcct gtggctgcgt gttcttcagc atacgaagac cagagccccc
     4201 cacacctgca tgtctgtgaa ctgctatttc agagactaca cgcagggtca tatcctacat
     4261 agtaataacc cctctatgta cagtcagaca cttccccaaa cacaggcagg ctctagtgtt
     4321 cacagaactg gcttccttaa gagttattct ccatcattct gtccctcttc tgccaaaatg
     4381 cttgtaacca ggcatgaggt accttatgtg ataaaatata aactgatcct tacatttaag
     4441 atatgtgtgc ttgtacttgc acacgcacac atgcacgcac atgctcacac actgctattg
     4501 cattctgggc catatgcaca tatagatatc caccccccac cttggaacac ttgggctccc
     4561 acattcctac ggcacaagca tgcgtattta gatctgcaca ggagtgttca atgataacct
     4621 ggtactacat gccagaagcc cgctgctgtt ctagaggaat tacttcctga acctggagcc
     4681 ccttatccca gagggaagcc tccagctccc actcctacct gagggtagca agtaaggaaa
     4741 cagcaggaga atgctcagag ccagacctgg tctcaggggc tgtatatatg gggcagcagg
     4801 gtgccccatc ccccacctgc acccagtccc tagccagatt tagaaagggc tgttgtcact
     4861 aaaagcattt cccccccgac tctccccccc ttcccttgtc caaacagtac tcccccctcc
     4921 tcccccagct aggaaacaat tggaagtggt cgtcattgtt gtggcatggc atgttcaggg
     4981 tctgagttcc agggggtggg gcagcttaaa gtgaagcaaa aatatctcta acattccacg
     5041 gctcactaga gagtaagctg accacgacca ccacgacctc aagcaggcaa ggccctggct
     5101 ggcctgcaag agggtcctgc tgaccctggc attgctgccc ccttagaagc cttgccccca
     5161 gtgctagcct ggccctattt ccagtatcct ccccacactc ctggcttgag tcctgcatct
     5221 gtctgaggtg ggaaggggtt gggtcacagc agctgagagg ggcagggcag agttgggggc
     5281 atggctctgg tatggtggtt ggattggagc agatagaagg tagtaggttc ctgttaggac
     5341 cagagagttt ggacacagga attctggctc acacactgct ttccttgggc cagcaggcaa
     5401 tgtgtccctg ggatcatggt gcttcagagc caggcagatc tttagtgatc catttaagtg
     5461 ctttgcttta gggggtgggg gtgggagatg cttcctctaa gattgcacag cttcagcgaa
     5521 gcaaaagtgg ggaacctcag cagataggaa gttgatccca atcaggggag gagggctagg
     5581 gatgctaaga agtatgccaa agctgtttgg ggaggaggac cagttgctga tggtcctgct
     5641 ttagagccct ttcctgttct agcgctgccc tgcagatttt cgaggcgcaa ggcctgagcc
     5701 tcccagggtt gcccagggct gtagatgata tggctacggc tgcctggtcc acctggcctc
     5761 tgtctagccg tgggtgcaat ctttgttctt cctagtggag agagggacag gacaggagag
     5821 accttctctt cccctgcagg ccccgggagc aacgctggca cccagcctcg ttcttgttct
     5881 tccagcatgg gctatggaag cttcactgct cggtgtctag gcaacagact cagggtttag
     5941 tttgaaatcc cctttcttcc caaacacagc tctgagcgca tgcggattaa tccagttgat
     6001 tagatgtcag tttgaaggag agaggcaggc ctgggggatg ggctgccagc aaagcaggta
     6061 cattgtgaag gtggggtagg catggcaggg aaaggaaaaa tccaggaagg gcttctgtgg
     6121 ggtttcttgg taaccaaagc aggggtaagg atgctgagca gctgtgtgca gtgtgcaggg
     6181 ggaaggcagg gggaaggtgg ggggaggacc acagactgta ggggctgcac ctcaatgcag
     6241 ctgcagctcc gcagtcctca gaatgcagtt gcagttgctg aagaactggt cttgcgaccc
     6301 tgccaggtat taatagagca gcctccctag gaggaacctc cagggccgga ggaacctcca
     6361 gggccgaagg aaggctaaag ctctcccagg ctcttctttt tttggggggg gggtggtttg
     6421 agacagggtt tctctgtata gccctggctg tcctggaact cactctgtag accaggctgg
     6481 cctcgaactt agaaatccac ctgcctctgc ctcccaagtg ctgggattaa aggcgtacca
     6541 ccaggctctc ccaggctctt ctagctttcc caacagaaga aggtgccttc ccgccatgct
     6601 ctgggagagg gctcaggcac aggtgcacgc actgagatgg ataaagaaag cttgggactc
     6661 aggcgagcca ttttaaagcc agtgtctttg agtggggcca acctgtgtgg ctgtgggatt
     6721 aggtagtcac ctctacaatt tagccacaca ccctttcccc cttctttcag gtttggaggg
     6781 ctttggtcta aaggtgtttt tggagtattg ttctcaactc tgcaaggcag aatttaaaag
     6841 ccagtttcct aattttaaga tctctgtctc atatcccaga tgtttggcct cctgcttgct
     6901 gctgttggcg gttgggtggc tcttcccatc ctttcctatg tgagtattca ttgagggcca
     6961 actttgagct tggcactgat gtggtcaatg gagatacgta acaatgaacc aaagcaaaga
     7021 cccctgctcc gggagctttg tccagctgat tgtagcagtg gacccaggct tcagagcaca
     7081 gcttgtgctt tgcatgcgtg cgtgacattc ctagtggaga gaatgggggc ccgaacccat
     7141 gtgaggagtg ggtggtgatg tggagcacac tacagagtga caggagacaa gacaaagggg
     7201 cagcaagtgc tataaataaa aagggaggct gctttgctga ggtcgaccaa ataaggcctg
     7261 gacagaagga aggtgatctg cctcccgagc ctcccctccc caggcattgt gcctgctttg
     7321 tctgggctct gtcaccacac tgatcaaatg tcaggtccaa ataggagcca tttgctgtga
     7381 acatgccagt ggagaccagc ggaaagtttg ctaaaattac tgctgggaat tagggggagg
     7441 tgggggtctg cagtcttgaa ctaaaggaga actgagcatg gaaagagaat tctgaaagga
     7501 atctcaaaac ccaaagggag aggctcagga ggaggcagaa gaggtagggc gtctggccca
     7561 aggggaggag gaggccttag ttgtctgaga acaggagagg ggacccagga gtggtagcca
     7621 gcacttgccc ctagctgcct gtatcccctg accttggaga acctatccct ggctccacat
     7681 gggccaggat tagggagctg agctagtctc tctcaggttt ctctttacaa gcagctgctc
     7741 cttcttgtca ttcttttgta ctccgctggt tccaaaatgc tttgttctct gcatactcaa
     7801 aacaccgccc actcaataca ccaggcaccc caatctcgac tttctgatag acagggacca
     7861 gctgggtagg cagaccagtt tcaataagag tctattaggc aggtagatct ctccttcaag
     7921 agtgtttgga gagggcactg tgtggagtgg ccagtctaga aacttgaggg agcttaaagg
     7981 ggggctaccg gggagcacag agatcccctt tgagaccttg ctccacagtt tctagctgta
     8041 tggtcagtca cataactccc tgagacagtt ttgccatcaa tgtggttttt tgcaaggatt
     8101 atggagacta aacacaggtg agggacctag cacacatcag cacataacag acttcataat
     8161 ggtttttatc tccaggaaca ccaacaccag ggacccagac tgcagacacc agagttagtt
     8221 tttttggccc ctagcatttc cctcagccca ccaagtctca ttggttgaag acaagacctt
     8281 cacgtgatcc tagacctaac ccatctccac aggatagcct tgagtcagga gaccactgcc
     8341 ctgctgctac ctataaaact tttctcacga ccatggtgaa ccccaagccc accaaaagct
     8401 ggcccagcaa aatggcaaca ttgtcaggaa aacaaatgga agtggagggg aagtgaagag
     8461 ttagctaaat gctttttgtg tctggggacc acacagaaag ctcctggtag agatggggag
     8521 ccaggttata aggaggagac aggttaaact gtggccctca ggaggtggtc cggccaggga
     8581 cccttcagat atttctgatc ttagggtcag ccaaggatgc agagggtctg gaaacctctg
     8641 aaggagaatg ctagagtatt ctgatatgga aaaaaaatgg gcaaacttag cttggtatag
     8701 gtctttaatt ccagcactca ggagacagaa gcatgcagat ctctgagttt gaggctagcc
     8761 tggtctatac agagaatgcc aggtcagtca gggagacaaa gtaagaccct gccagtgaga
     8821 gagagtctca ctgtgggact agcctgttaa ggcacccggc tttaaggacc aagtagctac
     8881 cataatctca gctcttggct gtgattctga tgctgttact atcttcaagc tgacttagac
     8941 aattctttag tatatgtctt agggtttcta ctgttgtgat aaaacaccat gatcagaagc
     9001 aacttgggag gaaagggttt acttcagccc acaactctca ggtcatcctc tgacactgag
     9061 ggaagccagg acaagaactc aaggtaagag tctggtggag gcaggggaac taagtctgct
     9121 tactggcttg ctccccaatg gcttgcccag tctgattctt gtaacaccca agcactcttg
     9181 cttaggggtg gcaccacctg caatgggctg tgctgtcctg tatcaataat taattaaaaa
     9241 gccaatctga tggggacatt ttttttcagt tgaggttcct cttcctcctc atatgatcct
     9301 agctgtgttg agcgaacaac aacaaaacta accaggacag tatgcatatt cttaaaagtg
     9361 ggcaatctgg agaagagcaa aggatggggt tagagagatg gctcactgtt tagagcactt
     9421 gctgttcttg caggggaatg gaggtctgat tctcacaacc cacagggcag catacaacct
     9481 tatgtaattc cagttccgag agatcctgtt gcatgctggc ctgcggctta caaggaccgg
     9541 gtacaccagg gaggcaggct ggggctgaaa gagacttaga tggcaagaca tgattctgct
     9601 caaggctctg gcctccaggt gtgggggagc tggcttttag aattagcaaa aaggccagag
     9661 attctctgga agtggaggct gtgctctgaa ttaactttgc taggtaatta agatttttag
     9721 ctatcttcaa ttggaatctc taatattgga cttattacta gaccagtcca agattgagtt
     9781 agaataactg gtcacattga aggaaaaagg agaatcatta taattctttc ttatgactaa
     9841 tctttctaag tcagattcca cagtctttga tgttcttttt accataaggt taaaaagctt
     9901 gaaacaaggc agctttgttt gtccaaactg gaaaaatggc aagcaaggat gggtcaaaaa
     9961 catgtaccca attcagcttc cttgtcacta cagtcactac agtcagggca ctgagcgaac
    10021 tcacctgtct tgtcacagga attagccatc atgcttctgc tccgttctgt ggagagaacc
    10081 tatgcttttc cctttcctaa taaggcagct gttcaggatc aaccatatgc caataagtag
    10141 atccttttag gatactataa agcatttatt aagttctttg acatccaaat ttcaaaattc
    10201 taaaataaaa gtatgattta aataatgtgc atgggaatgc actaaataac atgcacgggg
    10261 atataattgg atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
    10321 tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg
    10381 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    10441 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    10501 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    10561 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    10621 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10681 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10741 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10801 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10861 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10921 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10981 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11041 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    11101 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    11161 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    11221 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11281 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11341 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11401 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    11461 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    11521 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    11581 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    11641 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    11701 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    11761 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    11821 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    11881 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    11941 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    12001 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    12061 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    12121 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    12181 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    12241 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    12301 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    12361 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    12421 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    12481 gttccgcgca catttccccg aaaagtgcca c
//
