LOCUS       sequence-460770-       14956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses components of the LOOMINA optogenetic transcriptional 
            control system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14956)
  AUTHORS   Muench P, Fiumara M, Southern N, Coda D, Aschenbrenner S, Correia B,
            Graff J, Niopek D, Mathony J
  TITLE     A modular toolbox for the optogenetic deactivation of transcription.
  JOURNAL   Nucleic Acids Res. 2024 Dec 16:gkae1237. doi: 10.1093/nar/gkae1237.
  PUBMED    39676667
REFERENCE   2  (bases 1 to 14956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Dec 16:gkae1237. doi: 10.1093/nar/gkae1237."
COMMENT     Sequence Label: dCas9-AsLOV2x3-NLSx3
FEATURES             Location/Qualifiers
     source          1..14956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(37..56)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        228..607
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        609..807
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     762..782
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             825..1005
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1052..1177
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1670..1903
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2088..2132
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2430..2547
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2672..3850
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2903..3841
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3798..3818
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3869..3889
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3899..3925
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             3935..3955
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3965..3988,3989..3991,3992..6478,6479..6481,
                     6482..8065)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3965..8065
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    9474..10062
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9527..9547)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9945..9956)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10065..10081)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10066..10082)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10587..10767
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10793..10810)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10799..11023
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11233..11661
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11320..11339)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11530..11551
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11670..11690)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11675..12004
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11855..11990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11917..11936
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12052..12099
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12118..12492
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12622..12743
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12659..12678)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12713..12732
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12792..12808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12792..12808)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12805..12827)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12816..12832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12840..12846,12847..12864,12865..12870))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12885..12906
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13023..13040)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13194..13782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13274..13293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13953..14744,14745..14813))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14576..14595
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14814..14918)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
       61 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
      121 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
      181 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      241 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      301 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      361 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      421 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      481 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      541 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      601 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      661 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      721 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      781 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      841 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      901 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      961 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     1021 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     1081 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     1141 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     1201 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     1261 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     1321 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1381 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1441 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1501 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1561 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1621 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1681 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1741 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1801 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1861 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1921 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1981 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     2041 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     2101 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     2161 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     2221 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     2281 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     2341 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     2401 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2461 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2521 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2581 tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg
     2641 gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg
     2701 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
     2761 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     2821 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     2881 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
     2941 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct
     3001 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc
     3061 cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat
     3121 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaatttt
     3181 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga
     3241 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca
     3301 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta
     3361 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc
     3421 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc
     3481 cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga
     3541 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac
     3601 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc
     3661 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac
     3721 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt
     3781 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca
     3841 ggtgtcgtga cgtacggcca ccatgagccc caagaagaag aggaaagtcg gctctggccc
     3901 tgccgctaag agagtgaagc tggacggaag cggacccaag aagaagagaa aggtggaggc
     3961 cagcgacaag aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt
     4021 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
     4081 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga
     4141 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
     4201 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
     4261 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
     4321 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
     4381 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
     4441 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
     4501 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
     4561 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
     4621 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggcaa
     4681 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
     4741 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
     4801 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
     4861 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc
     4921 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg
     4981 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc
     5041 cggctacatt gacggcggag ccagccagga agagttctac aagttcatca agcccatcct
     5101 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg
     5161 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca
     5221 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat
     5281 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag
     5341 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga
     5401 agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa
     5461 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt
     5521 gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct
     5581 gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac
     5641 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat
     5701 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat
     5761 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt
     5821 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc
     5881 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg
     5941 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct
     6001 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga
     6061 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct
     6121 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac
     6181 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt
     6241 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag
     6301 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc
     6361 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg
     6421 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc
     6481 tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag
     6541 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa
     6601 gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct
     6661 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca
     6721 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa
     6781 cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc
     6841 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa
     6901 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa
     6961 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa
     7021 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag
     7081 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg
     7141 gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt
     7201 tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt
     7261 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat
     7321 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc
     7381 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt
     7441 gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga
     7501 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa
     7561 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact
     7621 gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag
     7681 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga
     7741 acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt
     7801 gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa
     7861 gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc
     7921 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa
     7981 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat
     8041 cgacctgtct cagctgggag gcgacggttc tggaagtgga tccggaagcg gacttgctac
     8101 aactttggag cggattgaga agaatttcgt cataactgat cccaggttgc ctgataatcc
     8161 tataatattc gcctccgata gttttcttca acttacggag tactccagag aggagatact
     8221 gggaaggaat tgcagattcc tccaaggtcc agaaactgat agagctactg tgaggaagat
     8281 acgggatgcc attgacaatc aaactgaagt gacagtgcaa cttatcaatt acaccaagtc
     8341 agggaaaaaa ttctggaacc tgttccattt gcaacccatg agggaccaaa agggcgatgt
     8401 gcaatatttc ataggcgttc aacttgatgg tactgaacac gtcagagatg cggcggagcg
     8461 agaaggagtg atgctgatta agaaaacagc agagaatatc gacgaagcgg cgaaagagtt
     8521 gagcgctgga ggaggtggaa gcggacttgc tacaactttg gagcggattg agaagaattt
     8581 cgtcataact gatcccaggt tgcctgataa tcctataata ttcgcctccg atagttttct
     8641 tcaacttacg gagtactcca gagaggagat actgggaagg aattgcagat tcctccaagg
     8701 tccagaaact gatagagcta ctgtgaggaa gatacgggat gccattgaca atcaaactga
     8761 agtgacagtg caacttatca attacaccaa gtcagggaaa aaattctgga acctgttcca
     8821 tttgcaaccc atgagggacc aaaagggcga tgtgcaatat ttcataggcg ttcaacttga
     8881 tggtactgaa cacgtcagag atgcggcgga gcgagaagga gtgatgctga ttaagaaaac
     8941 agcagagaat atcgacgaag cggcgaaaga gttgggttct ggaagtggat ccggaagcgg
     9001 acttgctaca actttggagc ggattgagaa gaatttcgtc ataactgatc ccaggttgcc
     9061 tgataatcct ataatattcg cctccgatag ttttcttcaa cttacggagt actccagaga
     9121 ggagatactg ggaaggaatt gcagattcct ccaaggtcca gaaactgata gagctactgt
     9181 gaggaagata cgggatgcca ttgacaatca aactgaagtg acagtgcaac ttatcaatta
     9241 caccaagtca gggaaaaaat tctggaacct gttccatttg caacccatga gggaccaaaa
     9301 gggcgatgtg caatatttca taggcgttca acttgatggt actgaacacg tcagagatgc
     9361 ggcggagcga gaaggagtga tgctgattaa gaaaacagca gagaatatcg acgaagcggc
     9421 gaaagagttg taaagcgaag tcaatcccag aattcgatat caagcttatc gataatcaac
     9481 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     9541 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     9601 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     9661 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     9721 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     9781 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     9841 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     9901 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     9961 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
    10021 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
    10081 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
    10141 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
    10201 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
    10261 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
    10321 accacacaca aggctacttc cctgattggc agaactacac accagtgcca gggatcagat
    10381 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
    10441 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
    10501 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
    10561 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
    10621 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    10681 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    10741 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
    10801 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
    10861 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
    10921 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
    10981 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
    11041 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcr
    11101 kkykkggkgg tkgggygggg caggacagca agggggagga ttgggaagac aatagcaggc
    11161 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
    11221 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
    11281 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
    11341 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    11401 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
    11461 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    11521 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
    11581 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
    11641 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
    11701 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
    11761 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    11821 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    11881 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    11941 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
    12001 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
    12061 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
    12121 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
    12181 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
    12241 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
    12301 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
    12361 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
    12421 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
    12481 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
    12541 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    12601 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
    12661 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    12721 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
    12781 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    12841 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    12901 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    12961 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    13021 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    13081 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    13141 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    13201 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    13261 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    13321 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    13381 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    13441 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    13501 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    13561 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    13621 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    13681 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    13741 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    13801 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    13861 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    13921 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    13981 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    14041 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    14101 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    14161 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    14221 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    14281 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    14341 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    14401 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    14461 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    14521 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    14581 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    14641 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    14701 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    14761 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    14821 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    14881 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    14941 cctgacgtcg acggat
//
