LOCUS       sequence-460843-       10834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gene trap RMCE plasmid with LexA[LexA(DBD)-linker-QFAD]-act-4 
            3'UTR::SEC cloned in the destination vector pHW1133 to swap driver 
            via RMCE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Khan H, Huang X, Raj V, Wang H
  TITLE     A versatile site-directed gene trap strategy to manipulate gene 
            activity and control gene expression in Caenorhabditis elegans.
  JOURNAL   PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 
            10.1371/journal.pgen.1011541. eCollection 2025 Jan.
  PUBMED    39841730
REFERENCE   2  (bases 1 to 10834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2025-01-22"; volume: "21(1):e1011541. doi"; pages: "
            10.1371/journal.pgen.1011541. eCollection 2025 Jan"
COMMENT     Sequence Label: pHW1223
FEATURES             Location/Qualifiers
     source          1..10834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     43..65
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     62..78
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     62..78
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     97..117
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        99..117
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    185..218
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             259..282
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2425..2458)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2468..2497
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(4314..4637)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6555..6579
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6582..7607
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6582..7607
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7608..7632)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7985..8018)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(8033..8066)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(8084..8100)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8085..8101)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(8125..8144)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8126..8144)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8154..8171)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8154..8170)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8163..8185)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8312..8767
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8399..8418)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8609..8630
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8793..8897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8898..8966,8967..9758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9116..9135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9929..10517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10418..10437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10671..10688
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10798..10819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10834,1..5,6..23,24..30)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
      121 caaaagctgg agctccaccg cggtggcggc cgctctagaa gggctgcagg aattcgatgc
      181 tagcgaagtt cctattctct agaaagtata ggaacttcta actaatctga tttaaatttt
      241 cagggtaccg gcggaagcga ctacaaggac gacgacgaca aggagccaac cacttgacta
      301 agtgataagc tagctaagct gtctcatcct actttcacct agttaactgc ttgtcttaaa
      361 atctatgctt ctctttagta tctaaaattt tcctagaagc ttacaagtat ataaatggtc
      421 tcttctcaat aaaggttgta tatttattca tcttattgaa tctgccattt cctcgttttt
      481 gcgagtttat ataccttcca attttctttc tattgtattt tcaacttcta attttaattc
      541 agggaaactg cttcaacgca tcaaaaatga aggccctcac cgcccgtcaa caagaggtct
      601 tcgacctcat ccgtgaccac atctcccaaa ccggaatgcc accaacccgt gccgagatcg
      661 cccaacgtct cggattccgt tccccaaacg ccgccgagga gcacctcaag gtaagtttaa
      721 acatatatat actaactaac cctgattatt taaattttca ggctctcgct cgtaagggag
      781 tcatcgagat cgtctccgga gcctcccgtg gaatccgtct cctccaagag gaggaggagg
      841 gactcccact cgtcggacgt gtcgctgctg gagagccact cctcgcccaa caacacatcg
      901 agggacacta ccaagtcgac ccatccctct tcaagccaaa cgccgacttc ctcctccgtg
      961 tctccggaat gtccatgaag gacatcggaa tcatggacgg agatctcctc gccgtccaca
     1021 agacccaaga cgtccgtaac ggacaagtcg tcgtcgcccg tatcgacgac gaggtcaccg
     1081 tcaagcgtct taaggtaagt ttaaacatga tattactaac taactaatct gatttaaatt
     1141 ttcagaagca aggaaacaag gtcgagctcc tcccagagaa ctccgagttc aagccaatcg
     1201 tcgtcgacct ccgtcaacaa tccttcacca tcgagggact cgccgtcgga gtcatccgta
     1261 acggagactg gctcgagctc gcgggttctg gatctggatc tggaggttcc ggtcgtcaac
     1321 ttgagctccc accaaccgcc accgccaccg ccagtatcat gccacacgtc atggagcaac
     1381 cactctccac ctccatcaac ccagtcaacg accgtttcaa cggaatacca aacccaaccc
     1441 catacaactc cgacgccgcc ctcgacgcca tcacccaaac caacgactac ggatccgtca
     1501 acacccacgg aatcctctcc acctacccac caccagccac ccacctcaac gaggcctccg
     1561 tcgccctcgc accaggagga gcaccaccac gtccaccacc accatacgtc gactccacca
     1621 ccaaccaccc accataccat tccaacctcg tcccaatggc caacttcgga tactccaccg
     1681 tcgactacga cgccatggtc gacgacctcg cctccatcga gtacaccgac gccgtcgacg
     1741 tcgacccaca attcatgacc aacctcgggt tcgtcccagg atgcaacttc tccgacatca
     1801 acacctatga gcaataaaag taaatttttt gccccttcca cccacaacgc tcaaaagaag
     1861 ttcgtattat cccgcaaccc atccagacca tccttttaca ttttttttat tttctctttt
     1921 ttttttcttc accctctccg tgtacattgc tagtaatagt tccaacttct ctagccgact
     1981 gcattatgct tcccattctc acccaaatct tcattggtaa cttctcttgt atttgttcaa
     2041 tactattccg gtagtaatct aggcatgtat aactaggttc tctgtgtatc tttggtttga
     2101 agttgaggtt taatgcaatc acttttgttg ttgtacccta ttactgccga tgacccccat
     2161 tttctcatta caaaattgac attttaaacc attttttctc tgaatcatgc tcattatttg
     2221 agccacattc ataataaagc ttttaataag acaaattgat caagtttatc taatgtaaaa
     2281 aatacaaata aagaaaattc aacccggagg agagatgaag tgagtagctg ttacggaaca
     2341 cagattattt gccttgtttg tttccgaaga tgtctccgac gcggtccaaa attgagccaa
     2401 tactgagcgg aaggtaagtt taaaataact tcgtatagca tacattatac gaagttattt
     2461 tcagggagag caaaagctta tctccgagga ggacctctaa ggatgatcga cgccaacgtc
     2521 gttgaatttt caaattttaa atactgaata tttgtttttt ttcctattat ttatttattc
     2581 tctttgtgtt ttttttcttg ctttctaaaa aattaattca atccaaatct aaacattttt
     2641 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag
     2701 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa
     2761 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc
     2821 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaacta
     2881 gctgccctcg attgtctatc ttccattctc accaagtttc aacaacttta ttcagtcatt
     2941 cttctctggt tcatttccca accaatcttc ccgaataaag cccatctgtc ctttcggcca
     3001 cattcctcac ctacatccgt actccttatc tcccgttctt atccagtctt ttatcgtctc
     3061 tgcgtctcgc acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact
     3121 tgcggaaaga tttcctaata aaaggagacg aatccggtag gaaatcgaca agtctctgct
     3181 tctcgaagac taggtgagtt acatttcttt ggccgaatta ttttgaaatc ctaaatttga
     3241 taatttcaga gatgtctgta aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca
     3301 ctcttatggt atgcttcatg accatgtcaa ccatctactc ggaggttgat ggattcagag
     3361 agaagcttga taccgagatg aatgtgttca gagtgagtta aattatattt tgaattttaa
     3421 ataattttta attttctagc aatctaccaa tggattgtgg aaggacatag ttgtcatcgg
     3481 aagatctagc aagcgtgtcc gttgtcaata tgaagagacc aacgctaccc caactccaca
     3541 tgctgatgga tccccatctg ctccaccagg tcaaccacca gcagttccac cagtcttcaa
     3601 ccagccaaag actccaaatg gagccaatgg aaatggacca acctgcaact gcaatgctga
     3661 taacaagtgc ccagctggac catccggacc aaagggagtt ccaggagttc caggactcga
     3721 cggagttcca ggacttgacg gtgttccagg agttggagct gatgatatcg ctccacaacg
     3781 cgagtctgtc ggatgcttca cttgcccaca aggaccagtt ggaccaccag gagctcttgg
     3841 aagaccagga ccacgtggac ttccaggacc aagaggacaa aatggaaacc caggaagaga
     3901 tggacaacca ggacatccag gagagcaagg atcatccggt caaatcggaa agatcggaga
     3961 gccaggacca ccaggagaga agggacgcga cgccgagcat ccaatcggaa gaccaggacc
     4021 aaagggacca agaggagatc aaggaccaac aggaccagct ggacagaacg gtcttcacgg
     4081 accaccagga gagccaggaa ccgttggacc agaaggacca tctggaaagc aaggacgtca
     4141 aggaccagac ggaacccagg gagagactgg accagacgga agaccaggaa aggatgccga
     4201 gtactgccag tgcccagaca agtctccacc atcagaggct gtcaacgcca accgtggata
     4261 cagaaatatc taaattgttg gtgttttcta ataaaatatt tgagattcag tgagactttt
     4321 ttcttggcgg cacaataaag tttcctcttt ttgttgggtc actcaacgaa cacaatacaa
     4381 atatttgagc gctcgcattt ttctcaacct ttcgggttta aagcacttca aaatggaatt
     4441 gaagagagat tgtgaaagta catcagacag aatcacattt tgatagcatt cacttcactc
     4501 agatgcaaac ttttcagtga ctataaaaag tcataaaata ataacattaa aaaagcaaat
     4561 aaattagcga gagaattttt tgacaaaaag aaagaagagt gatagagaag aagggaatgc
     4621 ttgaaaggat tttgcattta tcttaatcac cgtcttcgag aagacgaacc atggcgccag
     4681 tttcagaatc caagtttcta atatagttca tcacaatgtt gacgttggtc caacctccgg
     4741 cttgcataat ctctgggatg gaaactccag cgcgagccat gtcacgggca gctccgacac
     4801 gggcggaatg tccagaccaa gccaagtaac gttgtccgga atcatccttg gctccgtaga
     4861 tgagacggtg ggtagcttcg aagattcctg aaaatttatt ttcagcttat tcttagctaa
     4921 tatgttgttt aaacttacct tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg
     4981 cagcaactcc gttcttgcga acacgacaga acaggtagtt gtttggatca tcggcgactc
     5041 cggaaaccga aatccagcgc tcaacgagct tggtaactcc aagggagagc gctttttcga
     5101 ctccggcagt cgagaccagc gtttttgtac gtccgatgtg gatgagcatt cttcctccgt
     5161 ctgtgcgact aatatccttg acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt
     5221 tgtaagcaat tccgaggaag gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca
     5281 taagagaacg gacctgatcg aagtcggttc tgaaaatttc gtcaagtctt aggattgaat
     5341 acattaatat ttaaacttac ctttcgaagg cgagggcttg cttggctctc tctccagcat
     5401 caacattttc cttgcggata cgtctcatta cgagagatac ggcattagaa tctgatgggc
     5461 gtggaagtcc agatcggcga tggagcatat tgagctgtcc gagatgttgt tggatcgtct
     5521 tcaccgccaa tccacgagct tggaggtaga gcaagtagtc acgaacgtct tctggctcgg
     5581 ctgggaacca cttgcggttg ttaagcttgc accaggcagc ccaagaacgg cacactgaga
     5641 gaagcatctt ccaggtgtgc tcggagaagg cttgtctgtc acggaacata tccatgaggt
     5701 tcttgcgaac ttcatcactt gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag
     5761 tgagaagatt tgacatattt tcgaagtttt ttagatgcac tagaacaaag cgtgttggct
     5821 tcctctgagc ccgctttcct tatatacccg cattctgcag ccttacagaa tgttctagaa
     5881 ggtcctagat gcattcgttt gaaaatactc ccggtgggtg caaagagacg cagacggaaa
     5941 atgtatctgg gtctctttat tgtgtacact acttttccat gtaccgaatg tgagtcgccc
     6001 tccttttgca acaagcagct cgaatgttct agaaaaaggt ggaaaatagt ataaataccg
     6061 ttgaaaataa ataccgaaca acatttgctc taattgtgaa attagaaatc ttcaaactat
     6121 aatcatgtca ctttaccact atttccgtcc agctcaacgt tccgtttttg gtgatcattt
     6181 ttgctttcgt cgtaaatcta cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc
     6241 aaattcgttg actgagtatt caacgtttat acgttgtcgg caacgagaaa taggaaaatg
     6301 catcgggaaa tgttcttttt tcgatttttt ccaaggtttt gacaaatttt accacgaatt
     6361 ttgctatgtt ttcaattaaa aaatatgtta ttcaactgtt tctatgagga aaataaggct
     6421 ttgcatgtaa ttttcttatt cagcataatt tttaattaat ttgaattttc tgtcctaacg
     6481 tttattttgt tttcttggtt atgactgatc tgaaattaat ttttgaattt taaggtaata
     6541 tgtcaggcgg tgccgcaagt ttgtacaaaa aagcaggctc catgaaaaag cctgaactca
     6601 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     6661 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     6721 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     6781 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     6841 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     6901 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     6961 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     7021 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     7081 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     7141 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     7201 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     7261 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     7321 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     7381 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     7441 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     7501 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     7561 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagacc cagctttctt
     7621 gtacaaagtg ggtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc
     7681 accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt
     7741 ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa
     7801 aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca
     7861 caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat
     7921 agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt
     7981 tttaataact tcgtatagca tacattatac gaagttattt tcagggtccc gggaagttcc
     8041 tatactattt gaagaatagg aacttccccg ggatcaagct tatcgatacc gtcgacctcg
     8101 agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     8161 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     8221 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     8281 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     8341 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8401 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8461 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8521 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8581 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8641 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     8701 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     8761 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8821 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8881 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8941 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9001 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9061 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9121 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9181 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9241 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9301 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9361 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9421 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9481 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9541 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9601 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9661 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9721 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9781 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9841 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9901 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9961 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10021 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    10081 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10141 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10201 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10261 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10321 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10381 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10441 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10501 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10561 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10621 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10681 tgagcgagga agcggcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    10741 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    10801 tgtgagttag ctcactcatt aggcacccca ggct
//
