LOCUS       sequence-461136-       10853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 10853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pLIB-FLAG(N)-RPB1
FEATURES             Location/Qualifiers
     source          1..10853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(374..379,380..396,397..402))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(798..831)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        879..970
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     895..912
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1016..1039
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1046..1069
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      1609..1618
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2012..2021
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7112..7246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7149..7168)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7203..7222
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  7356..7521
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      7705..8159
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7792..7811)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8001..8022
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8185..8289
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8290..8358,8359..9150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8508..8527)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9321..9909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9810..9829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10063..10080
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  10214..10438
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(10505..10853,1..185))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca
       61 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt
      121 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt
      181 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
      241 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac
      301 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
      361 ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttt gtgccttcat
      421 ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct
      481 gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc
      541 cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc aggagatcgg
      601 tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat
      661 cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag
      721 tggttggcta cagctttgtt tgtactatca acaggttgaa ctgctgatca acagatcctc
      781 tacgcggccg cggtaccata acttcgtata gcatacatta tacgaagtta tctggtttta
      841 ctagtatcga ttcgcgacct actccggaat attaatagat catggagata attaaaatga
      901 taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa
      961 acctataaat attccggatt attcataccg tcccaccatc gggcgcggat ccatggacta
     1021 caaggacgac gatgacaagc tcgatcttga agtcctcttt cagggaccca tgcacggggg
     1081 tggccccccc tcgggggaca gcgcatgccc gctgcgcacc atcaagagag tccagttcgg
     1141 agtcctgagt ccggatgaac tgaagcgaat gtctgtgacg gagggtggca tcaaataccc
     1201 agagacgact gagggaggcc gccccaagct tggggggctg atggacccga ggcagggggt
     1261 gattgagcgg actggccgct gccaaacatg tgcaggaaac atgacagagt gtcctggcca
     1321 ctttggccac attgaactgg ccaagcctgt gtttcacgtg ggcttcctgg tgaagacaat
     1381 gaaagttttg cgctgtgtct gcttcttctg ctccaaactg cttgtggact ctaacaaccc
     1441 aaagatcaag gatatcctgg ctaagtccaa gggacagccc aagaagcggc tcacacatgt
     1501 ctacgacctt tgcaagggca aaaacatatg cgagggtggg gaggagatgg acaacaagtt
     1561 cggtgtggaa caacctgagg gtgacgagga tctgaccaaa gaaaagggcc atggtggctg
     1621 tgggcggtac cagcccagga tccggcgttc tggcctagag ctgtatgcgg aatggaagca
     1681 cgttaatgag gactctcagg agaagaagat cctgctgagt ccagagcgag tgcatgagat
     1741 cttcaaacgc atctcagatg aggagtgttt tgtgctgggc atggagcccc gctatgcacg
     1801 gccagagtgg atgattgtca cagtgctgcc tgtgcccccg ctctccgtgc ggcctgctgt
     1861 tgtgatgcag ggctctgccc gtaaccagga tgacctgact cacaaactgg ctgacatcgt
     1921 gaagatcaac aatcagctgc ggcgcaatga gcagaacggc gcagcggccc atgtcattgc
     1981 agaggatgtg aagctcctcc agttccatgt ggccaccatg gtggacaatg agctgcctgg
     2041 cttgccccgt gccatgcaga agtctgggcg tcccctcaag tccctgaagc agcggttgaa
     2101 gggcaaggaa ggccgggtgc gagggaacct gatgggcaaa agagtggact tctcggcccg
     2161 tactgtcatc acccccgacc ccaacctctc cattgaccag gttggcgtgc cccgctccat
     2221 tgctgccaac atgacctttg cggagattgt cacccccttc aacattgaca gacttcaaga
     2281 actagtgcgc agggggaaca gccagtaccc aggcgccaag tacatcatcc gagacaatgg
     2341 tgatcgcatt gacttgcgtt tccaccccaa gcccagtgac cttcacctgc agaccggcta
     2401 taaggtggaa cggcacatgt gtgatgggga cattgttatc ttcaaccggc agccaactct
     2461 gcacaaaatg tccatgatgg ggcatcgggt ccgcattctc ccatggtcta cctttcgctt
     2521 gaatcttagc gtgacaactc cgtacaatgc agactttgac ggggatgaga tgaacttgca
     2581 cctgccacag tctctggaga cgcgagcaga gatccaggag ctggccatgg ttcctcgcat
     2641 gattgtcacc ccccagagca atcggcctgt catgggtatt gtgcaggaca cactcacagc
     2701 agtgcgcaaa ttcaccaaga gagacgtctt cctggagcgg ggtgaagtga tgaacctcct
     2761 gatgttcctg tcgacgtggg atgggaaggt cccacagccg gccatcctaa agccccggcc
     2821 cctgtggaca ggcaagcaaa tcttctccct catcatacct ggtcacatca attgtatccg
     2881 tacccacagc acccatcccg atgatgaaga cagtggccct tacaagcaca tctctcctgg
     2941 ggacaccaag gtggtggtgg agaatgggga gctgatcatg ggcatcctgt gtaagaagtc
     3001 tctgggcacg tcagctggct ccctggtcca catctcctac ctagagatgg gtcatgacat
     3061 cactcgcctc ttctactcca acattcagac tgtcattaac aactggctcc tcatcgaggg
     3121 tcatactatt ggcattgggg actccattgc tgattctaag acttaccagg acattcagaa
     3181 cactattaag aaggccaagc aggacgtaat agaggtcatc gagaaggcac acaacaatga
     3241 gctggagccc accccaggga acactctgcg gcagacgttt gagaatcagg tgaaccgcat
     3301 tcttaacgat gcccgagaca agactggctc ctctgctcag aaatccctgt ctgaatacaa
     3361 caacttcaag tctatggtcg tgtccggagc taaaggttcc aagattaaca tctcccaggt
     3421 cattgctgtc gttggacagc agaacgtcga gggcaagcgg attccatttg gcttcaagca
     3481 ccggactctg cctcacttca tcaaggatga ctacgggcct gagagccgtg gctttgtgga
     3541 gaactcctac ctagccggcc tcacacccac tgagttcttt ttccacgcca tggggggtcg
     3601 tgaggggctc attgacacgg ctgtcaagac tgctgagact ggatacatcc agcggcggct
     3661 gatcaagtcc atggagtcag tgatggtgaa gtacgacgcg actgtgcgga actccatcaa
     3721 ccaggtggtg cagctgcgct atggcgaaga cggcctggca ggcgagagcg ttgagttcca
     3781 gaacctggct acgcttaagc cttccaacaa ggcttttgag aagaagttcc gctttgatta
     3841 taccaatgag agggccctgc ggcgcactct gcaggaggac ctggtgaagg acgtgctgag
     3901 caacgcacac atccagaacg agttggagcg ggaatttgag cggatgcggg aggatcggga
     3961 ggtgctcagg gtcatcttcc caactggaga cagcaaggtc gtcctcccct gtaacctgct
     4021 gcggatgatc tggaatgctc agaaaatctt ccacatcaac ccacgccttc cctccgacct
     4081 gcaccccatc aaagtggtgg agggagtcaa ggaattgagc aagaagctgg tgattgtgaa
     4141 tggggatgac ccactaagtc gacaggccca ggaaaatgcc acgctgctct tcaacatcca
     4201 cctgcggtcc acgttgtgtt cccgccgcat ggcagaggag tttcggctca gtggggaggc
     4261 cttcgactgg ctgcttgggg agattgagtc caagttcaac caagccattg cgcatcccgg
     4321 ggaaatggtg ggggctctgg ctgcgcagtc ccttggagaa cctgccaccc agatgacctt
     4381 gaataccttc cactatgctg gtgtgtctgc caagaatgtg acgctgggtg tgccccgact
     4441 taaggagctc atcaacattt ccaagaagcc aaagactcct tcgcttactg tcttcctgtt
     4501 gggccagtcc gctcgagatg ctgagagagc caaggatatt ctgtgccgtc tggagcatac
     4561 aacgttgagg aaggtgactg ccaacacagc catctactat gaccccaacc cccagagcac
     4621 ggtggtggca gaggatcagg aatgggtgaa tgtctactat gaaatgcctg actttgatgt
     4681 ggcccgaatc tccccctggc tgttgcgggt ggagctggat cggaagcaca tgactgaccg
     4741 gaagctcacc atggagcaga ttgctgaaaa gatcaatgct ggttttggtg acgacttgaa
     4801 ctgcatcttt aatgatgaca atgcagagaa gctggtgctc cgtattcgca tcatgaacag
     4861 cgatgagaac aagatgcaag aggaggaaga ggtggtggac aagatggatg atgatgtctt
     4921 cctgcgctgc atcgagtcca acatgctgac agatatgacc ctgcagggca tcgagcagat
     4981 cagcaaggtg tacatgcact tgccacagac agacaacaag aagaagatca tcatcacgga
     5041 ggatggggaa ttcaaggccc tgcaggagtg gatcctggag acggacggcg tgagcttgat
     5101 gcgggtgctg agtgagaagg acgtggaccc cgtacgcacc acgtccaatg acattgtgga
     5161 gatcttcacg gtgctgggca ttgaagccgt gcggaaggcc ctggagcggg agctgtacca
     5221 cgtcatctcc tttgatggct cctatgtcaa ttaccgacac ttggctctct tgtgtgatac
     5281 catgacctgt cgtggccact tgatggccat cacccgacac ggagtcaacc gccaggacac
     5341 aggaccactc atgaagtgtt cctttgagga aacggtggac gtgcttatgg aagcagccgc
     5401 acacggtgag agtgacccca tgaagggggt ctctgagaat atcatgctgg gccagctggc
     5461 tccggccggc actggctgct ttgacctcct gcttgatgca gagaagtgca agtatggcat
     5521 ggagatcccc accaatatcc ccggcctggg ggctgctgga cccaccggca tgttctttgg
     5581 ttcagcaccc agtcccatgg gtggaatctc tcctgccatg acaccttgga accagggtgc
     5641 aacccctgcc tatggcgcct ggtcccccag tgttgggagt ggaatgaccc caggggcagc
     5701 cggcttctct cccagtgctg cgtcagatgc cagcggcttc agcccaggtt actcccctgc
     5761 ctggtctccc acaccgggct ccccggggtc cccaggtccc tcaagcccct acatcccttc
     5821 accaggtggt gccatgtctc ccagctactc gccaacgtca cctgcctacg agccccgctc
     5881 tcctgggggc tacacacccc agagtccctc ttattccccc acttcaccct cctactcccc
     5941 tacctctcca tcctattctc caaccagtcc caactatagt cccacatcac ccagctattc
     6001 gccaacgtca cccagctact caccgacctc tcccagctac tcacccacct ctcccagcta
     6061 ctcgcccacc tctcccagct actcgcccac ctctcccagc tactcaccca cttcccctag
     6121 ctactcgccc acttccccta gctactcgcc aacgtctccc agctactcgc cgacatctcc
     6181 cagctactcg ccaacttcac ccagctattc tcccacttct cccagctact cacctacctc
     6241 tccaagctat tcacccacct cccccagcta ctcacccact tccccaagtt actcacccac
     6301 cagcccgaac tattctccaa ccagtcccaa ttacacccca acatcaccca gctacagccc
     6361 gacatcaccc agctattcac ctactagtcc caactacaca cctaccagcc ctaactacag
     6421 cccaacctct ccaagctact ctccaacatc acccagctat tccccgacct caccaagtta
     6481 ctccccttcc agcccacgat acacaccaca gtctccaacc tataccccaa gctcacccag
     6541 ctacagcccc agctcgccca gctacagccc aacctcaccc aagtacaccc caaccagtcc
     6601 ttcttacagt cccagctccc cagagtatac cccaacctct cccaagtact cacctaccag
     6661 tcccaaatat tcacccacct ctcccaagta ctcgcctacc agtcccacct attcacccac
     6721 caccccaaaa tactccccaa catctcctac ttattcccca acctctccag tctacacccc
     6781 aacctctccc aagtactcac ctactagccc cacttactcg cccacttccc ccaagtactc
     6841 gcccaccagc cccacctact cgcccacctc ccccaaaggc tcaacctact ctcccacttc
     6901 ccctggttac tcgcccacca gccccaccta cagtctcaca agcccggcta tcagcccgga
     6961 tgacagtgac gaggagaact gaaagcttgt cgagaagtac tagaggatca taatcagcca
     7021 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     7081 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     7141 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7201 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga
     7261 gcctagaaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     7321 ctgagcaata actatcataa cccctaggag atccgaacca gataagtgaa atctagttcc
     7381 aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc
     7441 tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc
     7501 caactatttt gtccgcccac agcggggcat ttttcttcct gttatgtttt taatcaaaca
     7561 tcctgccaac tccatgtgac aaaccgtcat cttcggctac tttttctctg tcacagaatg
     7621 aaaatttttc tgtcatctct tcgttattaa tgtttgtaat tgactgaata tcaacgctta
     7681 tttgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7741 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7801 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7861 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7921 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7981 tggagtccac gttcttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     8041 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     8101 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     8161 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8221 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8281 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8341 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8401 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8461 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8521 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8581 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8641 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8701 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8761 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8821 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8881 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8941 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9001 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9061 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9121 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9181 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9241 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9301 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9361 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9421 tttccgaagg taactggctt cagcagagcg cagataccaa atactattct tctagtgtag
     9481 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9541 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9601 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9661 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9721 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9781 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9841 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9901 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9961 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    10021 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    10081 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
    10141 cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatgcc
    10201 ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact gaacaaaata gatctaaact
    10261 atgacaataa agtcttaaac tagacagaat agttgtaaac tgaaatcagt ccagttatgc
    10321 tgtgaaaaag catactggac ttttgttatg gctaaagcaa actcttcatt ttctgaagtg
    10381 caaattgccc gtcgtattaa agaggggcgt ggccaagggc atggtaaaga ctatattcgc
    10441 ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga
    10501 attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca
    10561 actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat
    10621 aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct
    10681 gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct
    10741 caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag
    10801 caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag tcc
//
