LOCUS       sequence-461206-       11238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   DeCaen PG, Kimura LF
  TITLE     Methods to Assess Neuronal Primary Cilia Electrochemical Signaling.
  JOURNAL   J Cell Physiol. 2025 Apr;240(4):e70034. doi: 10.1002/jcp.70034.
  PUBMED    40227694
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Physiol."; date: "2025-04"; volume: "240(4):e70034. doi"; pages: " 
            10.1002/jcp.70034"
COMMENT     Sequence Label: pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}
FEATURES             Location/Qualifiers
     source          1..11238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2019..2322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2323..2526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2477..2497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2547..2571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3904..4566
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             4672..4728
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     5424..5443
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5467..5910
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     protein_bind    complement(5911..5935)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5953..6541
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6006..6026)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6424..6435)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6572..7071
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6607..6630)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7004..7023
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7088..7687
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7088..7107)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7584..7604
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7759..7992
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8064..8185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8101..8120)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8155..8174
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8225..8360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8380..8399)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8381..8399)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8409..8426)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8409..8425)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8418..8440)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8567..9022
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8654..8673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8864..8885
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9048..9152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9153..9221,9222..10013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9371..9390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10184..10772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10673..10692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10926..10943
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11060..11081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11096..11101,11102..11119,11120..11126)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11134..11150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11139..11161
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11158..11174
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11158..11174
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11193..11213
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11195..11213
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc tgccaccatg
     2581 ttcagtctga tggccagttg ctgcggctgg ttcaagcggt ggcgggagcc tgtcagaaag
     2641 gtgactcttt tgatggtggg acttgataat gctggtaaaa ccgcaacagc aaagggaatc
     2701 caaggagaat accctgaaga tgtagctcct actgttggat tttcaaaaat taaccttaga
     2761 caaggaaagt ttgaagtcac catctttgac ttgggaggtg gaataagaat tcggggaatc
     2821 tggaagaatt actatgctga atcctatggg gtaatatttg ttgtggattc cagtgatgaa
     2881 gagagaatgg aagagacaaa agaggctatg tcagaaatgc taagacatcc taggatatcg
     2941 ggaaagccta tattggtgtt ggcaaataaa caagataaag aaggagcttt aggagaagct
     3001 gatgtcattg aatgtctatc tctggaaaaa ttggtcaatg agcacaagtg cctgtgtcag
     3061 atagaaccat gttcagcaat ctcggggtat ggaaagaaaa ttgacaagtc cattaaaaaa
     3121 ggcctttatt ggctgctaca tgttattgca agagactttg atgccttaaa tgaacgcatc
     3181 caaaaagaga caacagagca gcgtgctctt gaggaacaag agaaacaaga aagagctgaa
     3241 cgagtgcgaa aattacgaga agaaagaaaa caaaatgaac aggagcaggc tgaactcgat
     3301 ggaaccagtg gtctggctga gttggaccca gaaccaacga atcctttcca gccaatagca
     3361 tctgtaatca ttgagaatga aggaaaactt gaaagagaga aaaaaaacca aaaaatggag
     3421 aaagacagtg atggctgcca cctgaaacat aaaatggagc atgagcaaat agagacacaa
     3481 ggccaggtta atcacaatgg ccaaaaaaat aatgaatttg gactagtaga aaattataag
     3541 gaggcattaa cacagcagtt aaagaatgaa gatgagacag accggccatc attggaatca
     3601 gctaatggta aaaagaaaac taagaaacta agaatgaaaa ggaaccaccg ggtagaacca
     3661 cttaatatag atgactgtgc tcctgagagt ccaacgccac ccccaccccc tcctcctgtt
     3721 ggctggggaa cccctaaagt cactagactt ccaaaacttg agcctcttgg tgaaacacat
     3781 cataatgatt tctataggaa gccactgcct cccctggctg tgccacagcg acctaacagt
     3841 gatgctcatg atgtgatctc aggcggcgga ggcggcggaa tggagagcga cgagagcggc
     3901 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     3961 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     4021 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     4081 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     4141 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     4201 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     4261 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     4321 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     4381 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     4441 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     4501 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccattgcc
     4561 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     4621 tccaccggat ctcgcaaggg cggcggaggc ggcggaatgg tcgactcttc acgtcgtaag
     4681 tggaataagg caggtcacgc agtcagagct ataggtcggc tgagctcacc cgtggtttcc
     4741 gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaaggg gctgaggctg
     4801 aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa gaagcccgtg
     4861 cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca caacgaggac
     4921 tacaccatcg tggaacagtg cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     4981 gagctgtaca agggaggtac aggcgggagt ctggtgagca agggcgagga ggataacatg
     5041 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     5101 gagttcgaga tcgagggcga gggcgagggc cgcccctacg aggcctttca gaccgctaag
     5161 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     5221 atgtacggct ccaaggccta cattaagcac ccagccgaca tccccgacta cttcaagctg
     5281 tccttccccg agggcttcag gtgggagcgc gtgatgaact tcgaggacgg cggcattatt
     5341 cacgttaacc aggactcctc cctgcaggac ggcgtattca tctacaaggt gaagctgcgc
     5401 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggct
     5461 acgcgtgacc aactgactga agagcagatc gcagaattta aagaggcttt ctccctattt
     5521 gacaaggacg gggatgggac gataacaacc aaggagctgg ggacggtgat gcggtctctg
     5581 gggcagaacc ccacagaagc agagctgcag gacatgatca atgaagtaga tgccgacggt
     5641 gacggcacat tcgacttccc tgagttcctg acgatgatgg caagaaaaat gaatgacaca
     5701 gacagtgaag aggaaattag agaagcgttc cgcgtgtttg ataaggacgg caatggctac
     5761 atcggcgcag cagagcttcg ccacgtgatg acagaccttg gagagaagtt aacagatgag
     5821 gaggttgatg aaatgatcag ggtagcagac atcgatgggg atggtcaggt aaactacgaa
     5881 gagtttgtac aaatgatgac agcgaagtag acccagcttt cttgtacaaa gtggtgataa
     5941 tcgaattccg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6001 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6061 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6121 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6181 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6241 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6301 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     6361 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6421 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6481 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6541 catcgggaat tcccgcggtt cgaattctac cgggtagggg aggcgctttt cccaaggcag
     6601 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     6661 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     6721 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     6781 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     6841 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     6901 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     6961 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     7021 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctcacgt
     7081 ggccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     7141 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     7201 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     7261 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     7321 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     7381 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     7441 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     7501 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     7561 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     7621 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     7681 tgcctgaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7741 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7801 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7861 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7921 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7981 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     8041 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     8101 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8161 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     8221 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8281 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8341 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     8401 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     8461 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     8521 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     8581 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8641 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8701 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8761 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     8821 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8881 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     8941 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     9001 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     9061 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     9121 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     9181 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     9241 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     9301 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     9361 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     9421 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     9481 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9541 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9601 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9661 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9721 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9781 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9841 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9901 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9961 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    10021 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    10081 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    10141 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    10201 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    10261 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    10321 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10381 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10441 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10501 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10561 acctacagcg tgagctatga gaaagcgcca cgcttcccga agagagaaag gcggacaggt
    10621 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10681 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10741 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10801 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10861 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    10921 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    10981 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    11041 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    11101 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    11161 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    11221 agctggagct gcaagctt
//
