LOCUS       sequence-461241-       25417 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  Module for the constitutive expression of EGFP, p19 and the N. nambi
            luminescence pathway genes fused to a SF..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 25417)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 25417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4080
FEATURES             Location/Qualifiers
     source          1..25417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        777..1121
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(1195..1197,1198..1200,1201..1911)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1195..1197,1198..1200,1201..1911)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1240..1261)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1501..1520)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1848..1869
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      1947..2199
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3043..3387
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      4003..4255
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5099..5443
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(5813..5824)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      6344..6596
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7440..7784
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      9150..9402
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        10246..10590
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      15784..16036
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        16880..17224
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     RBS             17352..17360
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      18242..18494
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    18736..18760
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             20061..20690
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             21119..22192
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      22258..22452
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(22688..22710)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    22796..22936
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     22851..22870
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(22951..22968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(23122..23710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(23202..23221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(23798..24589)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    25375..25399
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagctcaat ggtaacttta ctctttattt aaccatacat ttttttttat ttttttcact
       61 ttgttcttca tccactattg ttctttgttc atcttgaaca aaagctccct ccttctttgt
      121 tcttcatcca ccattgttct tcatcaatca tttcgctgtc aggagactag agccaagctg
      181 atctcctttg ccccggagat caccatggac gactttctct atctctacga tctaggaaga
      241 aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa gattagcctc
      301 ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc ggcaggtctg
      361 atcaagacga tctacccgag taataatctc caggagatca aataccttcc caagaaggtt
      421 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt
      481 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg
      541 caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa ggccatggag
      601 tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga acagttcata
      661 cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac
      721 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
      781 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
      841 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
      901 aaaggaaagg ctatcgttca agatgcccct gccgacagtg gtcccaaaga tggaccccca
      961 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     1021 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     1081 ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa
     1141 ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt cgaaatggtg
     1201 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1261 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1321 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1381 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1441 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1501 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1561 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1621 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1681 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1741 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1801 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1861 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggacgagctg
     1921 taagcttgga atggatcttc gatcccgatc gttcaaacat ttggcaataa agtttcttaa
     1981 gattgaatcc tgttgccggt cttgcgacga ttatcatata atttctgttg aattacgtta
     2041 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     2101 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     2161 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattgcca agctaattct
     2221 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     2281 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     2341 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2401 caataatggg accgactcgc gctgtcagga gactagagcc aagctgatct cctttgcccc
     2461 ggagatcacc atggacgact ttctctatct ctacgatcta ggaagaaagt tcgacggaga
     2521 aggtgacgat accatgttca ccaccgataa tgagaagatt agcctcttca atttcagaaa
     2581 gaatgctgac ccacagatgg ttagagaggc ctacgcggca ggtctgatca agacgatcta
     2641 cccgagtaat aatctccagg agatcaaata ccttcccaag aaggttaaag atgcagtcaa
     2701 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     2761 tactattcca gtatggacga ttcaaggctt gcttcataaa ccaaggcaag taatagagat
     2821 tggagtctct aagaaagtag ttcctactga atcaaaggcc atggagtcaa aaattcagat
     2881 cgaggatcta acagaactcg ccgtgaagac tggcgaacag ttcatacaga gtcttttacg
     2941 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc tcgtctactc
     3001 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
     3061 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
     3121 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
     3181 cgttcaagat gcccctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     3241 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     3301 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     3361 aggaagttca tttcatttgg agaggactcc ggtattttta caacaattac cacaacaaaa
     3421 caaacaacaa acaacattac aatttactat tctagtcgaa atggaacgag ctatacaagg
     3481 aaacgacgct agggaacaag ctaacagtga acgttgggat ggaggatcag gaggtaccac
     3541 ttctcccttc aaacttcctg acgaaagtcc gagttggact gagtggcggc tacataacga
     3601 tgagaccaat tcgaatcaag ataatcccct tggtttcaag gaaagctggg gtttcgggaa
     3661 agttgtattt aagagatatc tcagatacga caggacggaa gcttcactgc acagagtcct
     3721 tggatcttgg acgggagatt cggttaacta tgcagcatct cgatttttcg gtttcgacca
     3781 gatcggatgt acctatagta ttcggtttcg aggagttagt atcaccgttt ctggaggctc
     3841 tcgaactctt cagcatctct gtgagatggc aattcggtct aagcaagaac tgctacagct
     3901 tgccccaatc gaagtggaaa gtaatgtatc aagaggatgc cctgaaggta ctgaaacctt
     3961 cgaaaaagaa agcgagtgag cttggaatgg atcttcgatc ccgatcgttc aaacatttgg
     4021 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgacgattat catataattt
     4081 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     4141 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     4201 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     4261 ttgccaagct aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4321 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4381 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4441 accctgataa atgcttcaat aatgggaccg actcgcgctg tcaggagact agagccaagc
     4501 tgatctcctt tgccccggag atcaccatgg acgactttct ctatctctac gatctaggaa
     4561 gaaagttcga cggagaaggt gacgatacca tgttcaccac cgataatgag aagattagcc
     4621 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcggcaggtc
     4681 tgatcaagac gatctacccg agtaataatc tccaggagat caaatacctt cccaagaagg
     4741 ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat
     4801 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
     4861 ggcaagtaat agagattgga gtctctaaga aagtagttcc tactgaatca aaggccatgg
     4921 agtcaaaaat tcagatcgag gatctaacag aactcgccgt gaagactggc gaacagttca
     4981 tacagagtct tttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg
     5041 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg
     5101 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
     5161 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
     5221 ataaaggaaa ggctatcgtt caagatgccc ctgccgacag tggtcccaaa gatggacccc
     5281 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     5341 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
     5401 acccttcctc tatataagga agttcatttc atttggagag gactccggta tttttacaac
     5461 aattaccaca acaaaacaaa caacaaacaa cattacaatt tactattcta gtcgaaatga
     5521 gaattaacat ctccctttca tcattatttg aacgtctgtc aaagttatcc agcagatcca
     5581 ttgcaatcac ctgtggtgtc gtcttggcct ccgcaatcgc ttttccgata atccgtaggg
     5641 actatcagac ttttttggaa gtcgggccga gttacgctcc gcagaatttc agaggttaca
     5701 tcatagtatg cgtgctttca ctctttaggc aggaacaaaa agggttggct atctatgatc
     5761 gactgcccga aaagcgaagg tggttggcag atctcccatt ccgagaaggt acgcgtcctt
     5821 ctatcacaag tcatatcatc caaagacaga ggactcagct ggtagaccaa gaatttgcaa
     5881 cccgagaact cattgataaa gttataccca gagttcaggc tcgacacacc gataagacgt
     5941 ttctgtcaac ctcaaagttc gaatttcatg ccaaagcaat ttttttgctg cccagtattc
     6001 ccatcaatga tcccttgaat ataccttcac atgatactgt cagacgaaca aaaagggaga
     6061 tcgctcacat gcacgattac cacgactgca ccctgcactt ggctctcgca gcccaggacg
     6121 gtaaagaagt acttaaaaaa gggtggggcc agagacatcc cctcgctggc cccggcgtgc
     6181 cgggacctcc aaccgaatgg acgtttttat atgcaccgcg taacgaggag gaagccagag
     6241 tggtcgagat gatagtggag gccagtattg gttacatgac aaatgacccc gctggtaaaa
     6301 ttgtagagaa cgctaagtga gcttggaatg gatcttcgat cccgatcgtt caaacatttg
     6361 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgacgatta tcatataatt
     6421 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     6481 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     6541 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     6601 attgccaagc taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6661 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6721 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6781 aaccctgata aatgcttcaa taatgggacc gactcgcgct gtcaggagac tagagccaag
     6841 ctgatctcct ttgccccgga gatcaccatg gacgactttc tctatctcta cgatctagga
     6901 agaaagttcg acggagaagg tgacgatacc atgttcacca ccgataatga gaagattagc
     6961 ctcttcaatt tcagaaagaa tgctgaccca cagatggtta gagaggccta cgcggcaggt
     7021 ctgatcaaga cgatctaccc gagtaataat ctccaggaga tcaaatacct tcccaagaag
     7081 gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga gaaagatata
     7141 tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct tcataaacca
     7201 aggcaagtaa tagagattgg agtctctaag aaagtagttc ctactgaatc aaaggccatg
     7261 gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg tgaagactgg cgaacagttc
     7321 atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac
     7381 gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt
     7441 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
     7501 tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc
     7561 gataaaggaa aggctatcgt tcaagatgcc cctgccgaca gtggtcccaa agatggaccc
     7621 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     7681 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     7741 gacccttcct ctatataagg aagttcattt catttggaga ggactccggt atttttacaa
     7801 caattaccac aacaaaacaa acaacaaaca acattacaat ttactattct agtcgaaatg
     7861 gcatcctttg aaaatagtct gtccgttttg atcgtcgggg ctggccttgg gggattagca
     7921 gccgcaatag ctcttaggag acagggccat gtagtaaaga tctatgatag ttcctcattc
     7981 aaagctgaac tgggtgctgg ccttgctgta cctccgaata cgcttagaag ccttcaacag
     8041 ttaggttgta atacagagaa tttgaacgga gtcgacaatc tctgcttcac ggccatgggt
     8101 tacgacggct ccgtaggtat gatgaataac atgacagact acagggaggc ttatggcaca
     8161 tcctggatta tggttcatcg agtggattta cataatgaac ttatgcgtgt cgcacttgat
     8221 ccgggggggc tgggaccacc agcaacatta catttaaatc acagagtgac cttctgcgac
     8281 gttgacgcat gtacggtaac ttttaccaac gggacgacac aaagtgccga cttgatcgta
     8341 ggggctgacg gcatacgaag caccatacgt agattcgtct tggaggaaga tgtaactgtg
     8401 cctgccagcg ggatcgtcgg tttcagatgg ctggtgcaag ccgatgcctt agacccttac
     8461 cctgagttag attggattgt taagaagcct ccactcggtg ctcgtctgat ctctacgcca
     8521 cagaatccac agtccggcgt ggggttggct gaccgtcgta caattatcat ctacgcttgt
     8581 aggggtggca cgatggttaa tgtgttggct gtccacgacg acgaaagaga tcagaatacg
     8641 gcagattggt cagtgccggc ttccaaagat gatctcttcc gtgtattcca tgactaccac
     8701 ccaagattca gacgtttgct cgaacttgct caagatatca atctctggca aatgagggta
     8761 gttcccgtgc ttaaaaaatg ggtgaacaag cgtgtctgtt tattaggaga tgccgcccat
     8821 gcttctctcc ccacgctggg tcaagggttc ggaatgggac tggaggacgc cgtggcactc
     8881 ggcacattgt tacccaaagg aactacggct tcacagatcg aaacgagact ggccgtttac
     8941 gagcaattac gaaaggatag ggctgagttc gtagcagcag aatcctacga ggaacagtat
     9001 gtccctgaga tgcgtgggct ctatcttcgt tccaaagaac tccgagatcg agtgatggga
     9061 tatgatatta aagtggagtc agaaaaggtt ttggagactc tgcttcgatc ctctaattct
     9121 gcatgagctt ggaatggatc ttcgatcccg atcgttcaaa catttggcaa taaagtttct
     9181 taagattgaa tcctgttgcc ggtcttgcga cgattatcat ataatttctg ttgaattacg
     9241 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     9301 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     9361 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg ccaagctaat
     9421 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     9481 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9541 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9601 cttcaataat gggaccgact cgcgctgtca ggagactaga gccaagctga tctcctttgc
     9661 cccggagatc accatggacg actttctcta tctctacgat ctaggaagaa agttcgacgg
     9721 agaaggtgac gataccatgt tcaccaccga taatgagaag attagcctct tcaatttcag
     9781 aaagaatgct gacccacaga tggttagaga ggcctacgcg gcaggtctga tcaagacgat
     9841 ctacccgagt aataatctcc aggagatcaa ataccttccc aagaaggtta aagatgcagt
     9901 caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag
     9961 aagtactatt ccagtatgga cgattcaagg cttgcttcat aaaccaaggc aagtaataga
    10021 gattggagtc tctaagaaag tagttcctac tgaatcaaag gccatggagt caaaaattca
    10081 gatcgaggat ctaacagaac tcgccgtgaa gactggcgaa cagttcatac agagtctttt
    10141 acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca ctctcgtcta
    10201 ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca
    10261 aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa
    10321 aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc
    10381 tatcgttcaa gatgcccctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
    10441 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
    10501 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
    10561 ataaggaagt tcatttcatt tggagaggac tccggtattt ttacaacaat taccacaaca
    10621 aaacaaacaa caaacaacat tacaatttac tattctagtc gaaatgaact caagtaaaaa
    10681 tcctccttct actctccttg atgttttctt ggataccgca agaaacttgg atacagcaag
    10741 cagaaatgtg ctcgagtgtg gtgaacatcg atggagttac agagaactgg atacagtgtc
    10801 ctcagctctc gcccagcatc ttcgatacac ggtggggctg tcccctactg tcgcagtgat
    10861 cagcgagaat cacccgtaca tattggccct catgctcgct gtatggaaat tgggcggtac
    10921 cttcgctccg atagatgtcc acagccctgc agaactggtt gccgggatgc ttaacatagt
    10981 ctcaccgtct tgcctggtca tcccgtcatc agacgtaaca aaccagaccc ttgcatgtga
    11041 tcttaacatt ccagtggttg catttcatcc tcaccaaagc acaatcccgg aactgaacaa
    11101 aaagtacctg acggacagtc aaatcagccc tgatctccca ttccccgatc ccaaccgtcc
    11161 ggctctttac ctgttcactt cttctgctac gagtagatcc aacttgaagt gcgtgcccct
    11221 gacccacacc ttcatactga gaaatagcct gtcaaagagg gcctggtgca aaagaatgag
    11281 gccagaaaca gatttcgatg gtataagggt tttaggatgg gcaccatgga gtcatgttct
    11341 ggctcacatg caagatatag ggccccttac actcctgaat gctggatgtt acgtctttgc
    11401 aacgacaccg agcacatatc caaccgagtt gaaggacgat agggatgtca tttcttgcgc
    11461 cgcaaatgca gttatgtaca agggggttaa gtccttcgcc tgtctcccgt ttgtgctggg
    11521 aggccttaag gccctgtgtg agtccgagcc gtccgtcaaa gcacaattac aggtcgagga
    11581 gcgagcacag ttgttaaaga gcttacaaca catgaagatt cttgaatgtg gtggcgctat
    11641 gctcgaagtt tcagtggctt cttgggctat cgagaatcgt attcccatta gtataggaat
    11701 cggtatgacc gaaactggcg gagccttgtt cgctggaccc gttcaagcaa tccaaacggg
    11761 atttagttca gaggacaagt ttattgagga tgccacatat ctccttgtca aagacgatta
    11821 cgagtcacac gcagaagagg acatcaatga aggcgaactg gttgttaaaa gccgaatgct
    11881 gccaaggggt tatctggggt ataacgatcc atccttctca gtggacgatg cagggtgggt
    11941 taccttcaaa acaggcgaca ggtactccgt gaccccggat ggtaagtttt cttggttagg
    12001 aaggaataca gactttatac agatgacaag cggtgagact ctcgatccca ggcccatcga
    12061 aagtttgctt tgcgagtcct cccttatatc aagagcctgt gtaataggcg ataagttcct
    12121 gaatgggccc gccacagcag tgtgtgccat aatagaattg gagccgacta cggtagagaa
    12181 aggtcaggcc catagcaggg acatagcccg aatttttgcc cccataaacc gtgatttgcc
    12241 tcctccttta agaatcgcct ggagccacgt cctcgtgctc caacccagtg aaaaaatacc
    12301 gatgaccaaa aaaggaacga tcttccgtaa gaagatcgaa caagtctttg gtagtgcact
    12361 tggtgggtcc tcaggtgaca attcccaagc aaccactgat gcatcagtcg tccgtcgaga
    12421 tgaactgtca aacactgtta agcatatcat atcacgtgtt ctgggcgtga gcgacgacga
    12481 gttgctgtgg actctctcat ttgccgaact tggaatgacc tccgcccttg caacgagaat
    12541 tgccaatgaa ttgaatgagg ttttagtagg ggtcaatctg cctatcaatg catgttatat
    12601 acacgttgac ttaccatccc tgtcaaatgc agtatatgcc aaactggctc acttgaaact
    12661 ccctgacaga acaccagagc cgcgtaaggc accggttgag aatcctggcg gaaaagagat
    12721 cgtaatagtg ggacaagcat ttagacttcc aggctctatt aatgacgtgg ctagtctccg
    12781 tgacgctttt ctggcccgtc aagcaagttc aatcattaca gaaataccac cagacagatg
    12841 ggatcacgca tccttctacc cgaaggacat acgtttcaac aaggccggct tagtggacat
    12901 tgctaactac gaccacagtt tcttcggctt gacggctaca gaagctcttt acttatctcc
    12961 gaccatgaga ctcgccctgg aagttagttt tgaggccctt gagaacgcaa acattccggt
    13021 ttctcagctg aaggggtcac aaacagctgt ctacgttgct accacggatg acgggttcga
    13081 gactctctta aatgcagagg ctgggtacga cgcttacacc cgattctacg gtacggggcg
    13141 tgcagcttcc acggcatccg gcaggatctc atacctgctt gacgttcatg gacccagtat
    13201 aactgtggac acggcatgta gtggaggggc tgtttgcatt gatcaagcta tagactacct
    13261 gcaatccagt tcagccgctg ataccgccat catttgtgct tctaatactc attgctggcc
    13321 aggatcattc atgtttctga gcgcccaggg tatggtaagc tccggcggac gatgcgctac
    13381 tttcacgacc gatgccgacg gctacgtacc tagcgagggt gcagtcgcct tcattcttaa
    13441 aacacgtgaa gcagctatga gggacaaaga cacaattctg gctaccatca aagcaacaca
    13501 aatttctcat aatggtaggt cccagggatt agtcgccccc aatgtaaatt cccaagccga
    13561 cttgcataga agcctgctcc aaaaagccgg gctttcaccg gccgacattc atttcatcga
    13621 ggcacacgga actggaacct ccctcggaga tctgtccgag atccaagcta taaatgatgc
    13681 atatacgagc tcacaacctc gaacggccgg cccgctgatc gtttctgcaa gcaagacagt
    13741 gataggacac actgaaccag ccggtccgtt ggtggggatg ctgtcagtac tgaactcctt
    13801 taaagagggg gccgtccctg gtttggcaca cttgaccgcc gataacctga atcccgctct
    13861 tgattgcagc agtgttccgt tactcatacc ttaccagccc gttcacctgg cagcccccaa
    13921 gcctcatcgt gctgctgtaa tgtcatatgg attctctggc accctcggtg ggatcgtgct
    13981 ggaggcacca gatgaggaaa gactcgaaga ggaacctcct aatgacaaac ctatgttgtt
    14041 tgtagttagt gcaaaaacac atactgcatt aatagaatat ctcggccgat accttgaatt
    14101 cctcttgcag gcaaatccac aagacttctg cgatatttgt tatacttcat gcgttgggcg
    14161 tgaacattat cgatacagat tcgcatgcgt agccaacgac atggaggatt taataggaca
    14221 attgcagaag cgtttaggct ctaaggtccc accgaaacct tcctacaaaa gaggggccct
    14281 tgcattcgcc ttcagcggtc agggcacaca gttccgaggg atggctaccg agctcgcaaa
    14341 agcctattcc ggattccgaa aaatagtctc tgatctcgct aagagggcaa gtgaactctc
    14401 aggtcacgct attgatcgat ttttgttagc ttacgatatt ggggctgaaa acgtcgcccc
    14461 ggatagcgag gctgatcaaa tatgtatctt cgtatatcaa tgtagcgtgc ttcgttggct
    14521 tcagacaatg ggcattagac catctgctgt tatagggcac tccctcggtg agattagcgc
    14581 ttctgtcgcc gccggtgcat tgtctctgga ctctgcactg gaccttgtta tcagtcgagc
    14641 tcgactgtta aggagctcta ccaatgcacc cgccggtatg gcagccatgt cagcatcaca
    14701 ggacgaagtt gttgagttga ttgggaaatt agaccttgat aaagcaaatt cactctctgt
    14761 ttctgttatc aatggtcctc agaatacggt agtaagcggt tcttcagcag ccattgaaag
    14821 tatagtcgca ctggccaaag ggcgaaagat aaaagcaagt gctctcaaca tcaaccaagc
    14881 cttccactct ccctacgtag attctgcagt accaggtttg cgtgcttggt cagagaagca
    14941 tatttcctct gctagaccat tacagatccc cctgtactct acactccttg gcgcccaagt
    15001 aagtgaggga caaatgctga atccggatca ttgggtagat cacgcaagaa aacctgtcca
    15061 attcgctcag gccgccacca ttatgaaaga aagctttacc ggagtaatca ttgacatagg
    15121 tcctcaagta gttgcttgga gtttgctgct ctcaaacgga ctgacatcag ttaccgctct
    15181 cgccgcaaag cgaggacgat cacagcaggt tgccttcctt agtgctcttg cagatttata
    15241 ccaagactat ggtgtcgtgc ctgactttgt gggattatac gctcaacagg aggacgccag
    15301 tcgtttaaag aaaaccgata ttctgaccta tcctttccaa agggtgagaa ggtatccatc
    15361 tttcatcccg agtcgtaggg cacctactca tgcccatgtc caagatgagg aaacattgtc
    15421 atctggatct tccacaccca cattggagaa tacagatctc gactccggta aagagtcttt
    15481 gatgggaccc acacgtggcc ttcttcgagt agacgatctg agagacagta tcgtttcttc
    15541 tgtaaaggat gtactggagc tgaaatcaaa tgaggacttg gacttgtccg agtccttgaa
    15601 cgcattggga atggacagta ttatgttcgc tcaattacgt aaaaggatag gggagggact
    15661 cgggttatcc gtcccgatgg tattcctgtc tgacgccttc tcaatcggcg agatggttag
    15721 taaccttgta gaacaggcag aggctagtga ggacaactga gcttggaatg gatcttcgat
    15781 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
    15841 gcgacgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
    15901 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
    15961 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
    16021 tctatgttac tagatcggga attgccaagc taattcttga agacgaaagg gcctcgtgat
    16081 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
    16141 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    16201 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatgggacc gactcgcgct
    16261 gtcaggagac tagagccaag ctgatctcct ttgccccgga gatcaccatg gacgactttc
    16321 tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca
    16381 ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta
    16441 gagaggccta cgcggcaggt ctgatcaaga cgatctaccc gagtaataat ctccaggaga
    16501 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
    16561 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
    16621 aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc
    16681 ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg
    16741 tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct
    16801 tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
    16861 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
    16921 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
    16981 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc cctgccgaca
    17041 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
    17101 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
    17161 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
    17221 ggactccggt atttttacaa caattaccac aacaaaacaa acaacaaaca acattacaat
    17281 ttactattct agtcgaaatg gccccaataa gttccacgtg gtctaggctc ataaggtttg
    17341 tggctgtgga aacctcctta gttcacatcg gcgagcctat tgatgcaaca atggacgtgg
    17401 gcttagcacg tagggaaggg aagacaatac aagcctatga gatcatcggc agtggctctg
    17461 ctttagatct gtctgcccaa gtgtcaaaga acgtacttac ggtccgtgag ctcttaatgc
    17521 ccttgagccg tgaggagatt aaaacggtaa gatgccttgg ccttaattat cccgtccacg
    17581 ccacagaagc caacgtggcc gtgccgaaat tcccgaacct tttttacaaa cccgtaacct
    17641 ccctcatcgg accaggtggg ttgataacca tcccttcagt tgtccaacca cctaaagagc
    17701 accagagcga ttacgaagcc gagttggtta tcgtcatcgg taaagccgcc aaaaatgtca
    17761 gtgaagatga agctctggac tacgtgttgg gctacaccgc agccaatgac atctcattta
    17821 ggaaacatca gttggccgtt tctcaatggt cattctccaa gggttttgat ggcacgaatc
    17881 ccctgggacc gtgcctggtt tctgcttcct ctattccaga tccacaggat attccgatcc
    17941 aatgtaaact caacggtggg gtggtgcaaa atggaaacac tcgtgatcag atttttaatg
    18001 tcaagaaaac aatctctttt ttaagccaag ggactacgtt ggagcctggt tccataatcc
    18061 tgacggggac gcccgacgga gtgggcttcg taagaaaccc accgctttat ttgaaggacg
    18121 gtgatgaagt aatgacttgg atcggatcag gtataggaac cttagccaac actgtccgag
    18181 aggagcagac ttgtttcgct tcaggagggc acgagtaagc ttggaatgga tcttcgatcc
    18241 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
    18301 gacgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
    18361 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
    18421 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
    18481 tatgttacta gatcgggaat tgccaagcta attcttgaag acgaaagggc ctcgtgatac
    18541 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
    18601 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    18661 atccgctcat gagacaataa ccctgataaa tgcttcaata atgggaccga ctcgcgctgt
    18721 catgagaccg gatcctgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
    18781 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
    18841 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
    18901 ctatagtgca gtcggcttct gacgttcagt gcagccgtca tctgaaaacg acatgtcgca
    18961 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
    19021 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
    19081 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
    19141 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
    19201 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
    19261 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
    19321 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
    19381 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
    19441 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
    19501 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
    19561 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
    19621 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
    19681 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
    19741 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
    19801 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
    19861 ctctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
    19921 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
    19981 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
    20041 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
    20101 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
    20161 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
    20221 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
    20281 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
    20341 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
    20401 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
    20461 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
    20521 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
    20581 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
    20641 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
    20701 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
    20761 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
    20821 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
    20881 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
    20941 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
    21001 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
    21061 gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
    21121 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
    21181 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
    21241 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
    21301 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
    21361 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
    21421 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
    21481 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
    21541 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
    21601 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
    21661 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
    21721 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
    21781 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
    21841 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
    21901 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
    21961 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
    22021 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
    22081 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
    22141 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
    22201 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt
    22261 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
    22321 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
    22381 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
    22441 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
    22501 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
    22561 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
    22621 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
    22681 gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg taagcggatg
    22741 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
    22801 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
    22861 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    22921 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    22981 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    23041 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    23101 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    23161 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    23221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    23281 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    23341 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    23401 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    23461 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    23521 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    23581 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    23641 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    23701 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    23761 ctcacgttaa gggattttgg tcatgcattc taggtgatta tttgccgact accttggtga
    23821 tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat
    23881 cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg
    23941 gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg
    24001 cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcacca gcccagtcgg
    24061 gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa
    24121 ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt
    24181 ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa
    24241 tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa
    24301 tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag
    24361 gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc
    24421 ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg
    24481 cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa
    24541 ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcata atgtttaact
    24601 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc
    24661 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa
    24721 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
    24781 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagctta cgaaccgaac
    24841 aggcttatgt ccactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
    24901 ccttgggtag cagcgaagtc gaggcatttc tgtcctggct ggaacagaac ttattatttc
    24961 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
    25021 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
    25081 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
    25141 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
    25201 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
    25261 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
    25321 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
    25381 gatatattgt ggtgtaaaca taacggatcc ggtctca
//
