LOCUS       sequence-461412-         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of N-terminally 6His tagged hnRNPA1 D262N LCD 
            (186-320).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Das T, Zaidi F, Farag M, Ruff KM, Messing J, Taylor JP, Pappu RV, 
            Mittag T
  TITLE     Metastable condensates suppress conversion to amyloid fibrils.
  JOURNAL   bioRxiv [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 
            10.1101/2024.02.28.582569.
  PUBMED    38464104
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 
            10.1101/2024.02.28.582569."
COMMENT     Sequence Label: A1-LCD D262N
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ctcgaatcaa caagtttgta caaaaaagca
       61 ggcttcgaaa acctgtattt tcagggcagc atggccagcg ccagcagcag ccagagaggc
      121 agaagcggca gcggcaattt cggcggagga agaggcggcg gattcggcgg caacgacaac
      181 tttggcagag gcggcaactt cagcggcagg ggcggatttg gaggctctag aggcggaggc
      241 ggctatggcg gaagcggcga cggctataac ggcttcggca acgatggctc caacttcgga
      301 ggcgggggaa gctacaacaa tttcggcaac tacaacaacc agagcagcaa cttcggcccc
      361 atgaagggcg gaaactttgg cggcagaagc agcggaccgt atggcggagg gggacagtac
      421 ttcgccaagc ccagaaacca gggcggctac gggggatcta gcagcagctc tagctacggc
      481 tccggcagac ggttctaa
//
