LOCUS       sequence-46154-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of CaMKII-driven stabilized step function opsin 
            (SSFO) for optogenetic activation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, 
            Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R, 
            Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K
  TITLE     Neocortical excitation/inhibition balance in information processing 
            and social dysfunction.
  JOURNAL   Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360.
  PUBMED    21796121
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-07-27- 27"; volume: "477"; issue: "7363"; 
            pages: "171-8"
COMMENT     Sequence Label: pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      225..234
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagttaccgg ggcaacggga acagggcgtt tcggaggtgg ttgccatggg gacctggatg
       61 ctgacgaagg ctcgcgaggc tgtgagcagc cacagtgccc tgctcagaag ccccaagctc
      121 gtcagtcaag ccggttctcc gtttgcactc aggagcacgg gcaggcgagt ggcccctagt
      181 tctgggggca gctctagagc ggtaccggat ccccgggtac cggtgccacc atggactatg
      241 gcggcgcttt gtctgccgtc ggacgcgaac ttttgttcgt tactaatcct gtggtggtga
      301 acgggtccgt cctggtccct gaggatcaat gttactgtgc cggatggatt gaatctcgcg
      361 gcacgaacgg cgctcagacc gcgtcaaatg tcctgcagtg gcttgcagca ggattcagca
      421 ttttgctgct gatgttctat gcctaccaaa cctggaaatc tacatgcggc tgggaggaga
      481 tctatgtgtg cgccattgaa atggttaagg tgattctcga gttctttttt gagtttaaga
      541 atccctctat gctctacctt gccacaggac accgggtgca gtggctgcgc tatgcagagt
      601 ggctgctcac ttctcctgtc atccttatcc acctgagcaa cctcaccggc ctgagcaacg
      661 actacagcag gagaaccatg ggactccttg tctcagccat cgggactatc gtgtgggggg
      721 ctaccagcgc catggcaacc ggctatgtta aagtcatctt cttttgtctt ggattgtgct
      781 atggcgcgaa cacatttttt cacgccgcca aagcatatat cgagggttat catactgtgc
      841 caaagggtcg gtgccgccag gtcgtgaccg gcatggcatg gctgtttttc gt
//
