LOCUS       sequence-46166-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical 
            inhibition..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R,
            Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K
  TITLE     Principles for applying optogenetic tools derived from direct 
            comparative analysis of microbial opsins.
  JOURNAL   Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808.
  PUBMED    22179551
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-18- 18"; volume: "9"; 
            issue: "2"; pages: "159-72"
COMMENT     Sequence Label: pLenti-CaMKIIa-eArch 3.0-EYFP
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..325)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(116..137)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     465..484
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattctcat tacacctcgt
       61 tctcgtagca gaacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      121 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggt
      181 agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      241 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
      301 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
      361 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      421 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      481 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      541 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcaggccg tagccgaagg
      601 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
      661 tcagcttgcc gta
//
