LOCUS       sequence-461754-        8612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive 
            promoter. Its sgRNAs X.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Pujar A, Pathania A, Hopper C, Pandi A, Calderon CR, Fugger M, Nowak
            T, Kushwaha M
  TITLE     Phage-mediated intercellular CRISPRi for biocomputation in bacterial
            consortia.
  JOURNAL   Nucleic Acids Res. 2024 Dec 27:gkae1256. doi: 10.1093/nar/gkae1256.
  PUBMED    39727169
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Dec 27:gkae1256. doi: 10.1093/nar/gkae1256."
COMMENT     Sequence Label: pSEVA47_dCas9-GFP_AAN_4
FEATURES             Location/Qualifiers
     source          1..8612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        98..173
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        278..353
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        401..435
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             546..554
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             560..1273
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(1477..1503,1504..1506,1507..3993,3994..3996,
                     3997..5583)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    5587..5607
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      5662..5756
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             5992..6783
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            6929..7037
                     /note="incP origin of transfer"
     CDS             complement(7228..8178)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      8226..8448
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(join(8549..8612,1..23))
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gactgagcct ttcgttttat ttgatgcctt taattaatag ctttacaccc tagcgtagct
       61 ttggtattat gctagcttcc tcgcagaagg agaatacgtt ttagagctag aaatagcaag
      121 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
      181 ccacctctac ttatccgagt gttgcgttgg agacagaaga gtttacaccc cgcgtttata
      241 gtggtattat gctagctatt tccgtgcctg ggtatgagtt ttagagctag aaatagcaag
      301 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
      361 tacgcagtaa gtctgtagag gcgatttccc aaccagtgct ttgacagcta gctcagtcct
      421 aggtataatg ctagctccat ccgagtgttt tggtgtaaag ccagtattct ccttctgcga
      481 ggagccatca tacccaggca cggaaatatg gcggcgcgat tatccgcgcc ttctctagga
      541 ctttttaagg aggtatttca tgcgtaaagg cgaagaactg tttaccggtg tggttccgat
      601 tctggtggaa ctggacggcg atgttaatgg tcataaattc agtgttcgcg gcgaaggtga
      661 aggcgatgcg acgaacggca aactgaccct gaaatttatc tgcaccacgg gtaaactgcc
      721 ggtcccgtgg ccgacgctgg tgaccacgct gacctatggc gttcaatgtt ttgcgcgtta
      781 cccggatcac atgaaacagc acgacttttt caaatcggcc atgccggaag gctatgtgca
      841 ggaacgtacg attagcttta aagacgatgg tacgtataaa acccgcgcgg aagtgaaatt
      901 cgaaggcgat accctggtta accgtatcga actgaaaggt atcgatttca aagaagacgg
      961 caatattctg ggtcataaac tggaatataa cttcaattcc cacaacgtgt acatcaccgc
     1021 ggataaacag aaaaacggca ttaaagccaa tttcaaaatc cgccataatg tggaagatgg
     1081 tagcgttcag ctggccgacc actatcagca aaacacgccg attggtgatg gcccggtcct
     1141 gctgccggac aatcactacc tgagtaccca gtccgtgctg tcaaaagatc cgaacgaaaa
     1201 acgtgaccac atggtcctgc tggaatttgt gacggctgcg ggtatcaccc acggcatgga
     1261 cgaactgtat aaaaggcctt aataatactc gaacccctag cccgctctta tcgggcggct
     1321 aggggttttt tgtcctagag acgaacaata aggcctccca aatcgggggg ccttttttat
     1381 tgataacaaa acctaggcca ggcatcaatt gaaatcatca aactcattat ggatttaatt
     1441 taaactcaag ggacatcagg aggagggaat tatagaatgg ataagaaata ctcaataggc
     1501 ttagctatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata taaggttccg
     1561 tctaaaaagt tcaaggttct gggaaataca gaccgccaca gtatcaaaaa aaatcttata
     1621 ggggctcttt tatttgacag tggagagaca gcggaagcga ctcgtctcaa acggacagct
     1681 cgtagaaggt atacacgtcg gaagaatcgt atttgttatc tacaggagat tttttcaaat
     1741 gagatggcga aagtagatga tagtttcttt catcgacttg aagagtcttt tttggtggaa
     1801 gaagacaaga agcatgaacg tcatcctatt tttggaaata tagtagatga agttgcttat
     1861 catgagaaat atccaactat ctatcatctg cgaaaaaaat tggtagattc tactgataaa
     1921 gcggatttgc gcttaatcta tttggcctta gcgcatatga ttaagtttcg tggtcatttt
     1981 ttgattgagg gagatttaaa tcctgataat agtgatgtgg acaaactatt tatccagttg
     2041 gtacaaacct acaatcaatt atttgaagaa aaccctatta acgcaagtgg agtagatgct
     2101 aaagcgattc tttctgcacg attgagtaaa tcaagacgat tagaaaatct cattgctcag
     2161 ctccccggtg agaagaaaaa tggcttattt gggaatctca ttgctttgtc attgggtttg
     2221 acccctaatt ttaaatcaaa ttttgatttg gcagaagatg ctaaattaca gctttcaaaa
     2281 gatacttacg atgatgattt agataattta ttggcgcaaa ttggagatca atatgctgat
     2341 ttgtttttgg cagctaagaa tttatcagat gctattttac tttcagatat cctaagagta
     2401 aatactgaaa taactaaggc tcccctatca gcttcaatga ttaaacgcta cgatgaacat
     2461 catcaagact tgactctttt aaaagcttta gttcgacaac aacttccaga aaagtataaa
     2521 gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt atattgatgg gggagctagc
     2581 caagaagaat tttataaatt tatcaaacca attttagaaa aaatggatgg tactgaggaa
     2641 ttattggtga aactaaatcg tgaagatttg ctgcgcaagc aacggacctt tgacaacggc
     2701 tctattcccc atcaaattca cttgggtgag ctgcatgcta ttttgagaag acaagaagac
     2761 ttttatccat ttttaaaaga caatcgtgag aagattgaaa aaatcttgac ttttcgtatt
     2821 ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt ttgcatggat gactcggaag
     2881 tctgaagaaa caattacccc atggaatttt gaagaagttg tcgataaagg tgcttcagct
     2941 caatcattta ttgaacgcat gacaaacttt gataaaaatc ttccaaatga aaaagtacta
     3001 ccaaaacata gtttgcttta tgagtatttt acggtttata acgaattgac aaaggtcaaa
     3061 tatgttactg aaggaatgcg aaaaccagca tttctttcag gtgaacagaa gaaagccatt
     3121 gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta agcaattaaa agaagattat
     3181 ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag gagttgaaga tagatttaat
     3241 gcttcattag gtacctacca tgatttgcta aaaattatta aagataaaga ttttttggat
     3301 aatgaagaaa atgaagatat cttagaggat attgttttaa cattgacctt atttgaagat
     3361 agggagatga ttgaggaaag acttaaaaca tatgctcacc tctttgatga taaggtgatg
     3421 aaacagctta aacgtcgccg ttatactggt tggggacgtt tgtctcgaaa attgattaat
     3481 ggtattaggg ataagcaatc tggcaaaaca atattagatt ttttgaaatc agatggtttt
     3541 gccaatcgca attttatgca gctgatccat gatgatagtt tgacatttaa agaagacatt
     3601 caaaaagcac aagtgtctgg acaaggcgat agtttacatg aacatattgc aaatttagct
     3661 ggtagccctg ctattaaaaa aggtatttta cagactgtaa aagttgttga tgaattggtc
     3721 aaagtaatgg ggcggcataa gccagaaaat atcgttattg aaatggcacg tgaaaatcag
     3781 acaactcaaa agggccagaa aaattcgcga gagcgtatga aacgaatcga agaaggtatc
     3841 aaagaattag gaagtcagat tcttaaagag catcctgttg aaaatactca attgcaaaat
     3901 gaaaagctct atctctatta tctccaaaat ggaagagaca tgtatgtgga ccaagaatta
     3961 gatattaatc gtttaagtga ttatgatgtc gatgccattg ttccacaaag tttccttaaa
     4021 gacgattcaa tagacaataa ggtcttaacg cgttctgata aaaatcgtgg taaatcggat
     4081 aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact attggagaca acttctaaac
     4141 gccaagttaa tcactcaacg taagtttgat aatttaacga aagctgaacg tggaggtttg
     4201 agtgaacttg ataaagctgg ttttatcaaa cgccaattgg ttgaaactcg ccaaatcact
     4261 aagcatgtgg cacaaatttt ggatagtcgc atgaatacta aatacgatga aaatgataaa
     4321 cttattcgag aggttaaagt gattacctta aaatctaaat tagtttctga cttccgaaaa
     4381 gatttccaat tctataaagt acgtgagatt aacaattacc atcatgccca tgatgcgtat
     4441 ctaaatgccg tcgttggaac tgctttgatt aagaaatatc caaaacttga atcggagttt
     4501 gtctatggtg attataaagt ttatgatgtt cgtaaaatga ttgctaagtc tgagcaagaa
     4561 ataggcaaag caaccgcaaa atatttcttt tactctaata tcatgaactt cttcaaaaca
     4621 gaaattacac ttgcaaatgg agagattcgc aaacgccctc taatcgaaac taatggggaa
     4681 actggagaaa ttgtctggga taaagggcga gattttgcca cagtgcgcaa agtattgtcc
     4741 atgccccaag tcaatattgt caagaaaaca gaagtacaga caggcggatt ctccaaggag
     4801 tcaattttac caaaaagaaa ttcggacaag cttattgctc gtaaaaaaga ctgggatcca
     4861 aaaaaatatg gtggttttga tagtccaacg gtagcttatt cagtcctagt ggttgctaag
     4921 gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag agttactagg gatcacaatt
     4981 atggaaagaa gttcctttga aaaaaatccg attgactttt tagaagctaa aggatataag
     5041 gaagttaaaa aagacttaat cattaaacta cctaaatata gtctttttga gttagaaaac
     5101 ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa aaggaaatga gctggctctg
     5161 ccaagcaaat atgtgaattt tttatattta gctagtcatt atgaaaagtt gaagggtagt
     5221 ccagaagata acgaacaaaa acaattgttt gtggagcagc ataagcatta tttagatgag
     5281 attattgagc aaatcagtga attttctaag cgtgttattt tagcagatgc caatttagat
     5341 aaagttctta gtgcatataa caaacataga gacaaaccaa tacgtgaaca agcagaaaat
     5401 attattcatt tatttacgtt gacgaatctt ggagctcccg ctgcttttaa atattttgat
     5461 acaacaattg atcgtaaacg atatacgtct acaaaagaag ttttagatgc cactcttatc
     5521 catcaatcca tcactggtct ttatgaaaca cgcattgatt tgagtcagct aggaggtgac
     5581 tgataatact agtagcggcc gctgcaggca tgcaagcttg cggccgcgtc gtgactggga
     5641 aaaccctggc gactagtctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     5701 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccag
     5761 gggtccccaa taattacgat ttacgtattt aaatgaacct tgaccgaacg cagcggtggt
     5821 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     5881 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     5941 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     6001 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     6061 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     6121 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     6181 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     6241 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     6301 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     6361 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     6421 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     6481 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     6541 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     6601 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     6661 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     6721 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     6781 taagacaatt gtccttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt
     6841 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact
     6901 ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga
     6961 gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg
     7021 ctctgcgagg ctggccgtag gccggcctgg tgaacagctt tgaatgcacc aaaaactcgt
     7081 aaaagctctg atgtatctat cttttttaca ccgttttcat ctgtgcatat ggacagtttt
     7141 ccctttgata tgtaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca
     7201 ctgatagata caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc
     7261 tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catacttact
     7321 ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg ctgttaaagc
     7381 atcgtgtagt gtttttctta gtccgttatg taggtaggaa tctgatgtaa tggttgttgg
     7441 tattttgtca ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg
     7501 tctatctagt tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac
     7561 caatttcata ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt
     7621 aagcctttta aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag
     7681 gctaatctct atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc
     7741 ataaatcctc atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat
     7801 gaattttttt aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt
     7861 ttcgcttgag aacttggcat agtttgtcca ctggaagatc ttaaagcctt taaccaaagg
     7921 attcctgatt tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata
     7981 agcattttcc ctactgatgt tcatcatctg agcgtattgg ttataagtga acgataccgt
     8041 ccgttctttc cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat
     8101 tagcttggtt tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa
     8161 aacaactaat tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt
     8221 gagatgggct agtcaatgat aattactagt ccttttcctt tgagttgtgg gtatctgtaa
     8281 attctgctag acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc
     8341 tagacctttg tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa
     8401 agaataaaaa aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc
     8461 agttccgcag tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc
     8521 tggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca
     8581 aacaacagat aaaacgaaag gcccagtctt tc
//
