LOCUS       sequence-462033-        5047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2052m is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP1344 - pAAV-AiE2052m-minBG-iCre
FEATURES             Location/Qualifiers
     source          1..5047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      569..578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             581..601
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1650..2126
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1849..1868)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2166..2306
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2166..2295
                     /label=AAV2 ITR
     rep_origin      2381..2836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2468..2487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2678..2699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2853..2872)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2972..2994
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3032..3050)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3118..3222
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3223..3291,3292..4083)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3441..3460)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4254..4842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4743..4762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   4904..5033
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 atatggatgt cttacctgca tgaatacttg tgtaccacat gtatgccttt ctccatggag
       61 gccagaagag ggtatcagat tccctggaac tgaagttaca gatagttgtg agctgccatg
      121 tgggtactgg aaatacatgt gtctaatttt aaggaccagc tcctactgag gacataaatg
      181 ttatgtttct taaaagtcta taccataaga atcttcttca gatcttggtg tgaagattct
      241 taccccagat tggttacaga agcggtacca ggtgggtgga tcccatcaaa agctttagtg
      301 agaaatcacc cttttccttc acagcagccc agctgactgg tgaactttgc cgaactgtaa
      361 aatatcaaac ctttgggaag ctttctgcaa cacgagaagt tttaatcctt caatattaaa
      421 aagaaaaata agaaaaaaaa actgtggaga agtcacgaac aggagaaaca aaaaggaatt
      481 gtgcacaggg tcagtcgctt aaggagctcg ggctgggcat aaaagtcagg gcagagccat
      541 ctattgctta catttgcttc tgggatccgc caccatggtg cccaagaaga agaggaaagt
      601 ctccaacctg ctgactgtgc accaaaacct gcctgccctc cctgtggatg ccacctctga
      661 tgaagtcagg aagaacctga tggacatgtt cagggacagg caggccttct ctgaacacac
      721 ctggaagatg ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc tgaacaacag
      781 gaaatggttc cctgctgaac ctgaggatgt gagggactac ctcctgtacc tgcaagccag
      841 aggcctggct gtgaagacca tccaacagca cctgggccag ctcaacatgc tgcacaggag
      901 atctggcctg cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga ggagaatcag
      961 aaaggagaat gtggatgctg gggagagagc caagcaggcc ctggcctttg aacgcactga
     1021 ctttgaccaa gtcagatccc tgatggagaa ctctgacaga tgccaggaca tcaggaacct
     1081 ggccttcctg ggcattgcct acaacaccct gctgcgcatt gccgaaattg ccagaatcag
     1141 agtgaaggac atctcccgca ccgatggtgg gagaatgctg atccacattg gcaggaccaa
     1201 gaccctggtg tccacagctg gtgtggagaa ggccctgtcc ctgggggtta ccaagctggt
     1261 ggagagatgg atctctgtgt ctggtgtggc tgatgacccc aacaactacc tgttctgccg
     1321 ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc caactgtcca cccgggccct
     1381 ggaagggatc tttgaggcca cccaccgcct gatctatggt gccaaggatg actctgggca
     1441 gagatacctg gcctggtctg gccactctgc cagagtgggt gctgccaggg acatggccag
     1501 ggctggtgtg tccatccctg aaatcatgca ggctggtggc tggaccaatg tgaacattgt
     1561 gatgaactac atcagaaacc tggactctga gactggggcc atggtgaggc tgctcgagga
     1621 tggggactaa ggctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc
     1681 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa
     1741 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg
     1801 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac
     1861 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag
     1921 cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca
     1981 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca
     2041 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt
     2101 gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg
     2161 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     2221 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     2281 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
     2341 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
     2401 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
     2461 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2521 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2581 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2641 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2701 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     2761 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2821 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     2881 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     2941 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     3001 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     3061 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     3121 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3181 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3241 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3301 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3361 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3421 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3481 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3541 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3601 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3661 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3721 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3781 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3841 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3901 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3961 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4021 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4081 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4141 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4201 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4261 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4321 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4381 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4441 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4501 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4561 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4621 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4681 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4741 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4801 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4861 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc
     4921 gctcgctcgc tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt
     4981 gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg
     5041 cacgcgt
//
