LOCUS       sequence-462036-        5454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0486m is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle T, Dwivedi D, Way SW, Oster
            A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya X, 
            Roth JR, Allen S, Ayala A, Bakken TE, Barcelli T, Barta S, Bendrick 
            J, Bertagnolli D, Bowlus J, Boyer G, Brouner K, Casian B, Casper T, 
            Chakka AB, Chakrabarty R, Chance RK, Chavan S, Departee M, Donadio 
            N, Dotson N, Egdorf T, Gabitto M, Garcia J, Gary A, Gasperini M, 
            Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, Halterman C, 
            Helback O, Hockemeyer D, Huang C, Huff S, Hunker A, Johansen N, 
            Juneau Z, Kalmbach B, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, 
            Lee AY, Leibly M, Lenz GH, Liang E, Lusk N, Malone J, Mollenkopf T, 
            Morin E, Newman D, Ng L, Ngo K, Omstead V, Oyama A, Pham T, Pom CA, 
            Potekhina L, Ransford S, Rette D, Rimorin C, Rocha D, Ruiz A, 
            Sanchez REA, Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shulga L, 
            Sigler AR, Siverts LA, Somasundaram S, Stewart K, Szelenyi E, Tieu 
            M, Trader C, van Velthoven CTJ, Walker M, Weed N, Wirthlin M, Wood 
            T, Wynalda B, Yao Z, Zhou T, Ariza J, Dee N, Reding M, Ronellenfitch
            K, Mufti S, Sunkin SM, Smith KA, Esposito L, Waters J, Thyagarajan 
            B, Yao S, Lein ES, Zeng H, Levi BP, Ngai J, Ting J, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types (preprint).
  JOURNAL   bioRxiv [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 
            10.1101/2024.06.10.597244.
  PUBMED    38915722
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 
            10.1101/2024.06.10.597244."
COMMENT     Sequence Label: AiP1666 - pAAV-AiE0486m-minBG-IRES2-iCre-HGHpA
FEATURES             Location/Qualifiers
     source          1..5454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     misc_feature    517..1090
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    540..1092
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(684..701)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     911..930
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1116..1136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2201..2677
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2400..2419)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2717..2857
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2717..2846
                     /label=AAV2 ITR
     rep_origin      2932..3387
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3019..3038)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3229..3250
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3404..3423)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3523..3545
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3583..3601)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3669..3773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3774..3842,3843..4634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3992..4011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4805..5393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5294..5313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttcagg ccaatacctc agcgaaaatc aaatcagttg
      181 caaaatccat acagtcttcc agtttctcag ctgtggaaac tttgtgtctc ttcttttcca
      241 ccaaaacagc tatctcgtct ttcaagtcct tgctgaaaaa aaaaagtgtt aaatttttct
      301 caatttagac ttccagcaaa acagattctg cttgttgaaa ggcattggtg ttcatgagta
      361 agtgagtttc cattttattt cttcacaaat aagttacatg aagtacttat cttatggtac
      421 tagagttaac tgggatttct aaacacaggt gagctcgggc tgggcataaa agtcagggca
      481 gagccatcta ttgcttacat ttgcttctgg gatccgcccc tctccctccc ccccccctaa
      541 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
      601 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
      661 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
      721 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
      781 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
      841 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
      901 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
      961 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     1021 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     1081 acgatgataa tatggccaca gctagcacca tggtgcccaa gaagaagagg aaagtctcca
     1141 acctgctgac tgtgcaccaa aacctgcctg ccctccctgt ggatgccacc tctgatgaag
     1201 tcaggaagaa cctgatggac atgttcaggg acaggcaggc cttctctgaa cacacctgga
     1261 agatgctcct gtctgtgtgc agatcctggg ctgcctggtg caagctgaac aacaggaaat
     1321 ggttccctgc tgaacctgag gatgtgaggg actacctcct gtacctgcaa gccagaggcc
     1381 tggctgtgaa gaccatccaa cagcacctgg gccagctcaa catgctgcac aggagatctg
     1441 gcctgcctcg cccttctgac tccaatgctg tgtccctggt gatgaggaga atcagaaagg
     1501 agaatgtgga tgctggggag agagccaagc aggccctggc ctttgaacgc actgactttg
     1561 accaagtcag atccctgatg gagaactctg acagatgcca ggacatcagg aacctggcct
     1621 tcctgggcat tgcctacaac accctgctgc gcattgccga aattgccaga atcagagtga
     1681 aggacatctc ccgcaccgat ggtgggagaa tgctgatcca cattggcagg accaagaccc
     1741 tggtgtccac agctggtgtg gagaaggccc tgtccctggg ggttaccaag ctggtggaga
     1801 gatggatctc tgtgtctggt gtggctgatg accccaacaa ctacctgttc tgccgggtca
     1861 gaaagaatgg tgtggctgcc ccttctgcca cctcccaact gtccacccgg gccctggaag
     1921 ggatctttga ggccacccac cgcctgatct atggtgccaa ggatgactct gggcagagat
     1981 acctggcctg gtctggccac tctgccagag tgggtgctgc cagggacatg gccagggctg
     2041 gtgtgtccat ccctgaaatc atgcaggctg gtggctggac caatgtgaac attgtgatga
     2101 actacatcag aaacctggac tctgagactg gggccatggt gaggctgctc gaggatgggg
     2161 actaatgagg cgcgccgcgg ccgctgctcg agagatctac gggtggcatc cctgtgaccc
     2221 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
     2281 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
     2341 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt
     2401 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
     2461 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat
     2521 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag
     2581 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg
     2641 attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg
     2701 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     2761 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     2821 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     2881 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     2941 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3001 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3061 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3121 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     3181 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3241 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
     3301 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3361 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     3421 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3481 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3541 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     3601 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3661 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3721 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3781 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3841 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3901 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3961 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4021 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4081 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4141 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4201 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4261 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4321 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4381 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4441 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4501 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4561 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4621 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4921 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
