LOCUS       sequence-462124-       13239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan-neuronal expression of 'GUR-3' using a genomic fragment and 
            fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13239)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 13239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS3-rab-3P::AI::gur-3G::SL2::his-24::tagBFP
FEATURES             Location/Qualifiers
     source          1..13239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10701..10723)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10823..10842
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10920..10938)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11006..11110
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11111..11179,11180..11971)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11329..11348)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12142..12730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12631..12650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12884..12901
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13018..13039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13054..13059,13060..13077,13078..13084)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13092..13108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13097..13119
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13116..13132
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13116..13132
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctaacaact tggaaatgaa ataagcttgc atgcctgcag gcgagttttg actggctttc
       61 aaaaactgag tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag
      121 acatttttgc aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct
      181 ataatagttt ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt
      241 gaaagtatgc agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata
      301 taatctaggt taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc
      361 tttcaaatgg tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc
      421 ggatttattc caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt
      481 gaaaaaatga gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg
      541 agggttccgt gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag
      601 ccacactgtt ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag
      661 atgctttcat tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa
      721 ttatgcatga gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg
      781 tgtggtttaa tctgtgaaag ttttgaatct tctgtttgaa aaaaaaaaca tttttaattt
      841 gtatatacga gtgaattggt taggccctgg ttacgttttc cagcgaattc attcaaattt
      901 cttatttttt ctattttttc aattaaataa tttagttctc tatcacatgt gtatgatcaa
      961 aatttcattc ctaatcgtaa gcaaatttag gctcatccgt agtataattt tgttatgaag
     1021 ggcttcacag taaaagctca aactccaaca tttagcaact agcaaccaac aaaaaaccaa
     1081 agaaatgtca gttttgaaaa gtcgttattt ctttgtcttc tcacaattca cacatcatct
     1141 tctcgttttc ctccgttttt ttgtcgtgct tccttctttt ctttacacat ttttctaacc
     1201 tttttttaag tctatcctct cctaatcctt tgtactctct aatcagtttt gctggttgtg
     1261 ctgcacaatt tttatcagat ttatgcgcga aacaaaagcc tctcgcggag agctccgcca
     1321 aatccggtct tcttttttcc ttgcctatcg gatttcacaa acattcagtt tgtcacttga
     1381 cgatgtgttg gaggcattac tactaggaag tgttattcat attttttatt cgtttctttt
     1441 tggtgactaa acgaaatatt ccgaacgttt tcctgatttc aggcggcgga aacgaagaag
     1501 accacgaata atcaacaggc tgccattgca tccgccagaa gtcgagtgag ttctttgaaa
     1561 atctttatta gagttatttg tgacaatttg ccactttttt tttaatgttt aatgaatctg
     1621 taacgaaatt aataccaagg gtgtccaaca gaactatctg agcggttatt taatttttag
     1681 atagtatatt tcttccgatc gtaacataca tattttcaag catagtactg tgatttaagt
     1741 tacagttttc caatcagtct tgattttaat aaaaactttt caactgcaaa aatgcctctg
     1801 aagtattttt ctatattttt atagttgaat aattttttga ttggcacaaa gaccgccgag
     1861 atatgagccg ttaaagtaaa gctgcatgga ttcaatatgg tttgtatttg attggagtca
     1921 cttacatgga aatatttgta tttaaatctc atattttaaa acaagacaaa caccaaatta
     1981 aatttttatt aatctgaaca tttttcaatc gatttggtaa acaaattatt agatgtttcc
     2041 ctcaaaaatt gtaacaattc ctaaattgtg gcacttataa taacatcgtc ctgaattctc
     2101 aaaaaaaacc gaatgctaag agagatttcg tttaactggg acctgtaact gaattttcaa
     2161 atattggcag tttttgataa actgggaggg aaatcaaaaa ccaaaactac agtacccctc
     2221 gtgttataat aaattgtcat tcactccttt tttgttccca acatttgctc ccatctcctg
     2281 acgagaaaga acaattttat gagcacggga acccgtgggc gtccatccgt tcctcctgtg
     2341 caatttcagc ccccttcctc tacattctct gtgttcccgt ttttatcgtt ttatgggtat
     2401 cgggaacgga cagatcgctt ccatttgctt ctattccgtc ctcacaagac gtcccactaa
     2461 ctgattttct tctgatttct tttcactgaa aatgggaaag aaagaaaaag acgatgaatc
     2521 gacgacttct tctcaggaga aggtgaagaa gaaggataag gagaaagttc atctaaggct
     2581 gaaagataaa gtaaaaaaac ttagaaaatc taagtcgatt gcagtatctt ctccagctga
     2641 ttatatgact tgtcagtgtg aaccatgcaa aaaagtagct ctcgaggtaa taatttttaa
     2701 actcaatacg tagttaagtt tgaactcctt ttaatttact tatcatctaa tttaccgtca
     2761 caatagtact tccattagca attattttaa aattacatgc atagcttcaa tgttcgtttt
     2821 tttgcgttct catatcgtat gttgtctgcc tctcgaatac tgactgtccg actagaatgt
     2881 cccggtagga ttactgtacg gtcactggga ataaactgtt tctgaaatca atctgaaatt
     2941 ccgtagatct tcttggttca atttcttatt cctcaatctt tggacaacgt caccttcaga
     3001 gtcgtcgttg agtatcccat cattaactag tcaatctttc tctctttttc atttccccca
     3061 acttttctgc atattttttg acgacgacga cctcgacggc aacgagaata catttatcta
     3121 ttttttagta tagcatatat acattttcta cttttctcta acccatttca gacgtataga
     3181 ttttggtttt cgaatgcaac tttgagattt ttcaaagatc ttcagatggg agcagtggac
     3241 tgtctatttg atattcttaa aatgtactct ataaaagatg aaactgtaat gaccaattcc
     3301 ctacataaat atttttaact aattggtcgg atatttgggg atcagagaag tacttgtttt
     3361 tgcgatatta ataatgatat atttttctag taaaaaaatg ttaagcgatt tgtgaataat
     3421 taagttggct tgaaaaagat tttttgatag ctatgtattt tgttaattta aatcagattt
     3481 taaaacttta ttattgacta tttatatata ttatatatta tatattttca tggtttaatc
     3541 aaaatctatt aaattatttc taagatctct aaatatcgaa ttctctaaaa agcttcatca
     3601 acaaaagcat ttcaattatc taacacaatg agaataattc aagaactgaa atagaaagaa
     3661 atctagaaaa aaaagagagt gaggtgccct attaaatcgg tgctctcctc tctaacagaa
     3721 gcgcctacac gcacccaaga gagctctttt tctttctttt ttcggagcac acatacacgc
     3781 atacactcct cagaagtgta tagagaaaat gggagaagaa gcgaaagaag acgaagaagc
     3841 ggttgtgatg tatgcatcgg gcgtttctat ccctctccgt gagcaacgag ctagtcaacc
     3901 catcgatcca tttttgtgag cacacacaga gagagactca aacacactca cagcttcaca
     3961 ctgagctcca agagctctgt agagagaagc cagagtatgc tttcctcgat tttcctaacg
     4021 tcttcttctt ctgctttttt ctttttccca acgagttgta gtccactaac ttcaattttt
     4081 cctccagaga aagagtttta gagagacagc tgataaatac actagagaaa taaaaagaat
     4141 gataaaaatt tgctatttta atcagttccc tctcgtttct ctaagttttg ttgttgggtt
     4201 gggaaattaa aattggaaaa tggaaataac gtttttttct gtctaatctt gaattctcca
     4261 gacatttctt atttcctttt ttgttgcaag tttttcttcc ttctctaatt tttaatcaaa
     4321 gatatctcgc tatgaacttt gaatttttga tattttgatt ctataaatac ataaagtaat
     4381 ctaaatgctc ttttaaaata aatctacagt agccctattt tcagggatcc ccgggattgg
     4441 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     4501 gacccttgga gggtaccgag ctcagaaaaa atgacgataa cagcgtcgaa tacgttggag
     4561 ttcaaatgga cgtcaccaag gagctcgaga tcgtcattca gtaagttcac tggaatgaac
     4621 ctgtgcagcc ttttattttt caacattgtt tataggaaca acgacagatg cggaacagaa
     4681 aatatcaatt gacatgagta atacttactg tgatcaggta aatgatacag tgatggttcg
     4741 actctaaaaa ttactgagat ttcgaataac taaaaacagt tttttggaat gattaaataa
     4801 gtaaaaattc taaatgtatc aacaatacgt gttgttttga ataattgaac ataaaagaaa
     4861 ataagaggag acaaagtgtg ttaagttgtg aaattttgac tgaagcgaca cattttcatc
     4921 aaagggtaat aatatgaaaa ccataatagc cctacatttt tgtgggtgca ggtatatttg
     4981 agactgcacg cacggtgtgc ctagatttat ttttagtttc aaatattttt catatttccg
     5041 aacgaatagt ggaagcagca aaatgtattt gtagcatcaa gtatttctag ccattttgca
     5101 atttttatat tgttcagttc taatgaagat tgcagaaaaa aacggtaaca aacgcttatt
     5161 caagaacaaa ttgtatatgt tcaggttttg gggcctctgt actcctatat gatggtgttg
     5221 ggacttaatc acactcacag tagcgctcgg aatacaatgt ttaagtggcc attgacaatc
     5281 tacaattatc tcactctagc catattggta agctacatta tgtcctgatc ttcgattaaa
     5341 caagattgta ctttctctgc gtgtgtgtaa tacgagatgt ttataaaagt ttagcccagc
     5401 aagtggaaca ttttcaaatt tttccagaca gcggccacaa ttcgacgcat ttcacaaatc
     5461 aaacaaaaat cggcaacaaa tgaggaaaaa gatgctgctt ttcatgtgct aaacccgacg
     5521 tttgtgctca cgttgtgtca tgctttgcta atgttcaggt tgtgttttgc gcagcgttaa
     5581 gttggttatt aaactcaaac atgaacaaac aaatataacg atacatgaaa atcgcacaaa
     5641 gaatacatgg tttttgattc gcgggcataa gattgtctag tttgctcaaa agtatttgta
     5701 tttcgggaga taattatatt taaaaaattt tgaaatgtta tggaagtggt cgtttgatat
     5761 caagatcgaa caatttttta aagatatgtg tttcctatta gtatggtgta cccataagaa
     5821 ttgcaatctc taaacaatat ttttaatgat atgaacattt aatgatattc ttcattcatt
     5881 tttgaggatt ccttactaag tcacaaaaaa atcgtcgaat taggtatttt ctagatgcca
     5941 attcgaggtt tcaaaaagtg tcacacacaa gttgaatata ccttaataaa aacaagttac
     6001 ttccaatttg gaacaaacca tcaaaactta gtattgtagt ctcttacagt ctaatagtat
     6061 tccacgaaaa catttccggg aattcgtatg gcacgcaata ctattagaag gtaaatcaat
     6121 tttcataaat tataaaccaa aaaaaaatat tgatggaaaa ttattgtttg aacgtttctg
     6181 tagtctattt tattaattta ttgttttatc tcgtatatga cttgtttaat tgacttgcat
     6241 tactagacag tctcaatttc ctaaaaacga actagtttct aattttttga ataagacttt
     6301 agtttcatgg aaattaaggt ttattggaaa ccaatcacaa agctaacatc acaaatttat
     6361 tttaaatgat atgcgctttg tgttattctc atatttaaat ttcagtggtc ttgctgccgg
     6421 attccttttg ctaaagttgc aaaagcaaag agagaaaatg taccacgtga gtgaaattag
     6481 acacggttga ggtagatcac aagaccaaca gggcttgact tacaggtgtt agatcaaggg
     6541 ttgggtagga atagaaatga agaacatgac agtcatcatt tcaagctgaa taagttattt
     6601 atatcaatca gtttttcgtt tgcagctgca ttatcctttg tacaaattgg taacaaaaaa
     6661 gtatgactaa ttcgaaacca ttcgaaaaat ttccagctac aaaaatgaga tatcttgatc
     6721 tccctgacac tcctgatctg attaacagaa aaatctactt tgtgattctt gaaggatacg
     6781 tggtacggtt gatattcttt atgcttgcca aaaattttca aaattaagat tttcatcgct
     6841 tccagttgca tttcattagt cgcaatatta ttttttcaac tatgtagaat actccaattt
     6901 tcgataggac aactaataga agaaatggtg cctaaagaaa aagaagaatg cccactacca
     6961 gaacaatctt tacaacaaat tcacgatgtt caaattcatt accaagaaat ttccaatgct
     7021 aagctgtata tagaacagaa tttctcattt tcactgtttt atacctacgg ctgttgtatt
     7081 ccgctgacct gtctgttggg ctacattgct tttcgaaatg gaattcaggt agtgaaaatg
     7141 agcagcaata ccattgtttc atatcatcat ttaggctgat atggcggaaa ctttttctgt
     7201 ggcaatatgg ctcactaata ctatgttagc attgatgctt ttctcgattc cagcttttat
     7261 gattgctgaa gaggttagta cttataagta tgtatatgtt tgtttaaata catagatata
     7321 gttagagcag aactgagaaa attgagttag taaagttttt gtatttcttc aacttttgat
     7381 gttagctgaa caaaaattaa aaaacaacat aaattaaata taatgcaagc atatttgtga
     7441 acaagaaatg tgtacgtgga taaagtcgcg gtggaattat catttaaccg tgattgcatg
     7501 catataaatc ccacaatacg taaactcaat ttatttatga cagtttcaca attacgatca
     7561 gaaatttaaa ataaataggt tttcaaacac tattcttcag cgacaatatt tctgctaatt
     7621 gtcgctgata ttttatgttc gcaaacgcag taattcaaaa ttgatatcca aacattaagt
     7681 tgtacaaata aaaattaata attacagggc gacaaacttc taaccgcttc tttcaaaatg
     7741 tatcacgaaa ctctatgtga ggaacgtgat cttctagttt tggtgtgttc gttatctcaa
     7801 aaaaaaatcg atcaattcta ctttcagtct caaatgagtt ttctttcgtt tcaaatgcat
     7861 gctacaaagc ttactctcac cgccgggaat ttcttcatga tgaatcgcaa aattatgatc
     7921 agtgtacgtt taaaggcgtg cagctggttt ttagttgata tttaaaatat ttcagttgtt
     7981 ctctgcaatt ttcacatatt ttcttatttt ggtacagttt gatgctgaaa aggagcgagc
     8041 cggagaatgc aataatcaat ctcgtgtgct cattgtccaa ccacctgtgt aaggtaccgc
     8101 tgtctcatcc tactttcacc tagttaactg cttgtcttaa aatctatgct tctctttagt
     8161 atctaaaatt ttcctagaag cttacaagta tataaatggt ctcttctcaa taaaggttgt
     8221 atatttattc atcttattga atctgccatt tcctcgtttt tgcgagttta tataccttcc
     8281 aattttcttt ctattgtatt ttcaacttct aattttaatt cagggaaact gcttcaacgc
     8341 atcatgtctg attccgctgt tgttgccgcc gctgtcgagc caaaggtccc aaaggctaag
     8401 gccgccaagg ccgccaagcc agccgccaag gccgccaagc caaccaaagt tgccaaggcc
     8461 aaggctccag tcgctcaccc accatacatc aacatgatca aggaggccat caagcagctc
     8521 aaggaccgca aaggagcttc caagcaggca atcctcaagt tcatctccca aaactacaag
     8581 ctcggagaca atgtcatcca gatcaatgct catctccgtc aggctctcaa gcgtggtgtc
     8641 accagcaagg cccttgttca agccgccgga tccggagcca acggacgttt ccgtgtgcca
     8701 gagaaggccg ccgccgccaa gaagccagca gcagccaaga agccagcagc agccaagaaa
     8761 ccagcagccg ccaagaaagc cactggagag aagaaggcaa agaagccagc cgctgccaag
     8821 ccaaagaagg ccgccaccgg agataagaag gtcaagaagg ccaagagccc aaagaaggtt
     8881 gccaagccag ctgccaagaa ggtcgccaag tctccagcca agaaggccgc tccaaagaag
     8941 atcgccaagc cagctgccaa gaaggccgcc aagccagccg ccaaggccgc atgcatgtcc
     9001 gaactcatca aggagaacat gcacatgaag ctctacatgg agggaaccgt agacaaccac
     9061 cacttcaagt gcacctccga gggagaggga aagccatacg agggaaccca aaccatgcgt
     9121 atcaaggtcg tcgagggagg accactccca ttcgccttcg acatcctcgc cacctccttc
     9181 ctctacgggt ccaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     9241 tttcagacct tcatcaacca cacccaagga atcccagact tcttcaagca atccttccca
     9301 gagggattca cctgggagcg tgtcaccacc tacgaggacg gaggagtcct caccgccacc
     9361 caagacacct ccctccaaga cggatgcctc atctacaacg tcaagatccg tggagtcaac
     9421 ttcacctcca acggaccagt catgcaaaag aagaccctcg gatgggagac cttcaccgag
     9481 accctctacc cagccgacgg aggactagag ggacgtaacg acatggccct caagctcgtc
     9541 ggaggctccc acctcatcgc caacatcaag gtaagtttaa acagttcggt actaactaac
     9601 catacatatt taaattttca gaccacctac cgttccaaga agccagccaa gaacctcaag
     9661 atgccaggag tctactacgt tgactaccgt ctagagcgta tcaaggaggc caacaacgag
     9721 acctacgtcg agcaacacga ggtcgccgtc gcccgttact gcgacctccc atccaagctc
     9781 ggacacaagc tcaactaagc gccggtcgct accattacca acttgtctgg tgtcaaaaat
     9841 aataggggcc gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata
     9901 tttaaatttt cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac
     9961 atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac
    10021 ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt
    10081 agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc
    10141 cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt
    10201 ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc
    10261 atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca
    10321 tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca
    10381 agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt
    10441 gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg
    10501 caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat
    10561 aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca
    10621 atataccaaa cataactgtt tcctactagt cggccgtacg ggccctttcg tctcgcgcgt
    10681 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
    10741 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    10801 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    10861 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg
    10921 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
    10981 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    11041 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
    11101 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
    11161 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
    11221 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
    11281 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
    11341 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    11401 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    11461 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    11521 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    11581 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    11641 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    11701 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    11761 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    11821 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    11881 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    11941 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    12001 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    12061 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    12121 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    12181 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    12241 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    12301 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    12361 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    12421 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    12481 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    12541 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    12601 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    12661 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    12721 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    12781 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    12841 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    12901 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    12961 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    13021 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    13081 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    13141 cgccaagctg taagtttaaa catgatctta ctaactaact attctcattt aaattttcag
    13201 agcttaaaaa tggctgaaat cactcacaac gatggatac
//
