LOCUS       sequence-462175-        8874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the production of SARS-CoV-2 virus-like particles (VLPs) in the 
            '3-plasmid' system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Yang Q, Davidson BA, Pajic P, Chen X, Gokcumen O, Gao M, Neelamegham
            S
  TITLE     Tuning the tropism and infectivity of SARS-CoV-2 virus-like 
            particles for mRNA delivery.
  JOURNAL   Nucleic Acids Res. 2025 Feb 27;53(5):gkaf133. doi: 
            10.1093/nar/gkaf133.
  PUBMED    40037714
REFERENCE   2  (bases 1 to 8874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-02-27"; volume: "53(5):gkaf133. doi"; 
            pages: " 10.1093/nar/gkaf133"
COMMENT     Sequence Label: pcDNA3.1 N-T2A-M-IRES-E
FEATURES             Location/Qualifiers
     source          1..8874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        399..602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     553..573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     647..666
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        647..665
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1983..2036
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    join(2714..3288,3289..3291,3292..3300)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2881..2898)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3108..3127
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    3571..4159
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3624..3644)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4042..4053)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4249..4266)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4255..4479
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4525..4953
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4612..4631)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4822..4843
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4962..4982)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4967..5296
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5147..5282
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5209..5228
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5363..6157
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5417..5436)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6027..6046
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6331..6452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6368..6387)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6422..6441
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6501..6517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6501..6517)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6514..6536)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6525..6541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6549..6555,6556..6573,6574..6579))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6594..6615
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6732..6749)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6903..7491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6983..7002)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7662..8453,8454..8522))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8285..8304
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8523..8627)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8702..8721)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      601 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
      661 atagggagac ccaagctggc tagcgctggc tagcgtttaa acttaagctt gccaccatgt
      721 ctgacaacgg ccctcagaac cagcggaatg ccccaagaat caccttcggc ggcccctccg
      781 attctacagg ctccaaccag aatggagaga ggtccggagc acgctctaag cagcggagac
      841 cacagggcct gcccaacaat accgccagct ggttcaccgc cctgacacag cacggcaagg
      901 aggacctgaa gtttcccagg ggccagggcg tgcctatcaa caccaatagc tcccctgacg
      961 atcagatcgg ctactatagg agggcaacaa ggagaatccg gggaggcgac ggcaagatga
     1021 aggatctgtc ccccagatgg tacttctact atctgggcac cggacctgag gcaggactgc
     1081 catatggcgc caataaggac ggaatcatct gggtggcaac cgagggcgcc ctgaacacac
     1141 caaaggatca catcggcaca cgcaatcccg ccaacaatgc agcaatcgtg ctccagctgc
     1201 cacagggcac cacactgccc aagggctttt acgcagaggg cagcaggggc ggctcccagg
     1261 cctctagccg ctcctctagc cggtccagaa actcctctcg gaattctacc ccaggcagct
     1321 ccatgggcac aagccctgca agaatggcag gaaacggagg cgacgccgcc ctggccctgc
     1381 tgctgctgga tagactgaat cagctggagt ctaagatgag cggcaaggga cagcagcagc
     1441 agggacagac cgtgacaaag aagtctgccg ccgaggccag caagaagcca aggcagaagc
     1501 gcaccgccac aaaggcctac aacgtgaccc aggccttcgg caggcgcgga ccagagcaga
     1561 cacagggcaa ttttggcgac caggagctga tcaggcaggg caccgattat aagcactggc
     1621 ctcagatcgc acagttcgca ccaagcgcct ccgccttctt tggcatgagc aggatcggaa
     1681 tggaggtgac cccatccggc acatggctga cctacacagg cgccatcaag ctggacgata
     1741 aggaccctaa cttcaaggat caggtcatcc tgctgaacaa gcacatcgat gcctataaga
     1801 cctttccccc tacagagccc aagaaggaca agaagaagaa ggccgatgag acccaggccc
     1861 tgcctcagag acagaagaag cagcagaccg tgacactgct gccagccgcc gacctggacg
     1921 atttttccaa gcagctccag cagtctatgt ctagcgccga tagcacccag gggtccggag
     1981 gagagggcag aggaagtctc ctaacatgcg gtgacgtgga ggagaatcct ggcccaatgg
     2041 ccgactcaaa tgggaccatt actgtagagg agctgaaaaa actgttggag caatggaacc
     2101 ttgtgattgg atttctcttc ttgacatgga tttgtctcct gcaattcgca tacgcgaatc
     2161 gcaaccgatt cctctatatt ataaaactta tattcctttg gctcctgtgg cctgtgaccc
     2221 ttgcctgctt cgtactcgcg gcggtgtatc gaataaactg gataactggc ggcatagcaa
     2281 tagctatggc atgcctggta ggtttgatgt ggctctccta ttttatagcg tcattcagac
     2341 tttttgcacg cacgcgaagc atgtggtcat ttaaccctga aacaaatatc ttgcttaatg
     2401 tccccctgca tggcacaatt ttgacccgac cgctccttga atcagaattg gtgattggag
     2461 ccgtgatcct gaggggccac ttgaggatcg caggacatca tcttggtcgc tgtgatatta
     2521 aggatttgcc aaaagaaata actgtagcta ccagtcgaac tttgagctat tacaagcttg
     2581 gagcgtccca aagggtcgct ggcgatagtg gatttgcagc ctattcacgc tacaggatag
     2641 ggaactataa actgaataca gaccactcat catcttctga taacattgct ttgctcgtcc
     2701 agtaaggatc ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     2761 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     2821 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     2881 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     2941 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     3001 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     3061 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     3121 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     3181 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     3241 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc
     3301 cacaaggaga aattcgccgc caccatgtac agcttcgtat cagaagaaac cgggacactg
     3361 atcgtaaatt ctgtgctctt gtttctggca ttcgtcgtat ttctcctcgt cacactggca
     3421 attctgactg cattgaggct ttgcgcctac tgttgtaaca ttgtcaatgt atctctcgtg
     3481 aaaccctcat tctacgttta cagcagggtg aagaatctca attctagcag ggtgccggat
     3541 ctcctcgttc tttaactcga ccgactcgac aatcaacctc tggattacaa aatttgtgaa
     3601 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3661 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3721 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3781 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3841 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3901 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3961 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4021 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4081 ctgccggctc tgcggcctct tccgcatctt cgccttcgcc ctcagacgag tcggatctcc
     4141 ctttgggccg cctccccgcc tggaattaat tcgagctcgg tacctttaag accaatgact
     4201 tacaaggcag cctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     4261 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4321 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4381 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4441 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4501 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4561 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4621 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4681 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4741 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4801 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4861 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4921 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     4981 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5041 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5101 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5161 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5221 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5281 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     5341 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5401 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5461 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5521 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     5581 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5641 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5701 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5761 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5821 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     5881 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     5941 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6001 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6061 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6121 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6181 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6241 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6301 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6361 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6421 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6481 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6541 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6601 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6661 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6841 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7081 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7321 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7801 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8401 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8461 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8521 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8581 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8641 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     8701 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     8761 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     8821 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtac
//
