LOCUS       sequence-46219-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Singh A, Sweeney MF, Yu M, Burger A, Greninger P, Benes C, Haber DA,
            Settleman J
  TITLE     TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers.
  JOURNAL   Cell. 2012 Feb 17;148(4):639-50.
  PUBMED    22341439
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-02-17"; volume: "148(4)"; pages: "639-50"
COMMENT     Sequence Label: pLenti-PGK-NRAS(G12V)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(672..698)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(678..704)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(734..758)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgag cggccgccac tgtgctggat atcaaccact
       61 ttgtacaaga aagctgggtc ttacatcacc acacatggca atcccataca accctgagtc
      121 ccatcatcac tgctgttgag ttttttcatt cggtactggc gtatttctct taccagtgtg
      181 taaaaagcat cttcaacacc ctgtctggtc ttggctgagg tttcaatgaa tggaatcccg
      241 taactcttgg ccagttcgtg ggcttgtttt gtatcaactg tccttgttgg caaatcacac
      301 ttgtttccca ctagcaccat aggtacatca tccgagtctt ttactcgctt aatctgctcc
      361 ctgtagaggt taatatccgc aaatgacttg ctattattga tggcaaatac acagaggaag
      421 ccttcgcctg tcctcatgta ttggtctctc atggcactgt actcttcttg tccagctgta
      481 tccagtatgt ccaacaaaca ggtttcacca tctataacca cttgttttct gtaagaatcc
      541 tctatggtgg gatcatattc atctacaaag tggttctgga ttagctggat tgtcagtgcg
      601 cttttcccaa caccaactgc tccaaccacc accagtttgt actcagtcat ggatccgcta
      661 gaaggtcctc ccaggctggc atagtcaggc acgtcataag gatagctaga agccatggtt
      721 ctatctcctt cgaagcctgc ttttttgtac aaacttgttg atatctgcag aattccacca
      781 cactggacta gtcgacgaac cggtggatcc ccctggggag agaggtcggt gattcggtca
      841 acgagggagc cgactgccga cgtgcgctcc gga
//
