LOCUS       sequence-462389-        9125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone for CRASP-Seq experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Behera AK, Kim JJ, Kordale S, Damodaran AP, Kumari B, Vidak S, 
            Dickson E, Xiao MS, Duncan G, Andresson T, Misteli T, 
            Gonatopoulos-Pournatzis T
  TITLE     RNA-coupled CRISPR Screens Reveal ZNF207 as a Regulator of LMNA 
            Aberrant Splicing in Progeria.
  JOURNAL   bioRxiv [Preprint]. 2025 Apr 26:2025.04.25.648738. doi: 
            10.1101/2025.04.25.648738.
  PUBMED    40568141
REFERENCE   2  (bases 1 to 9125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Apr 26:2025.04.25.648738. doi: 
            10.1101/2025.04.25.648738."
COMMENT     Sequence Label: pLCHKO-CRASP
FEATURES             Location/Qualifiers
     source          1..9125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..507
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     426..444
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             529..1125
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(529..548)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1025..1045
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1135..1191
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1192..1806,1807..1812,1813..1848,1849..1851,1852..1887,
                     1888..1890,1891..1926,1927..1935)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="SRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPAD
                     ALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    1951..2539
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2004..2024)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2422..2433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2611..2844
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2922..3043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2959..2978)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3013..3032
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3083..3218
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3238..3257)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3239..3257)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3267..3284)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3267..3283)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3276..3298)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3425..3880
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3512..3531)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3722..3743
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3906..4010
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4011..4079,4080..4871)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4229..4248)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5042..5630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5531..5550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5784..5801
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5918..5939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5954..5959,5960..5977,5978..5984)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5992..6008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5997..6019
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6016..6032
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6016..6032
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6051..6071
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6053..6071
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6099..6325
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6326..6506
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6553..6678
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7171..7404
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7589..7633
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    7842..8066
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8072..8320
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8072..8092
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8243..8262
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     CDS             complement(8374..8397)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8534..8558)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8580..8598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8617..8635
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8654..8672
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8691..8709
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8728..8746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8765..8783
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8802..8820
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8839..8857
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    8936..9053
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccgggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcagctc acactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac
      541 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
      601 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
      661 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
      721 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
      781 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
      841 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
      901 ctggccaccg tcggtgtttc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
      961 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     1021 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     1081 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccggatc cggcgcaaca
     1141 aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc gtctagactg
     1201 gacaagagca aagtcataaa cggcgctctg gaattactca atggagtcgg tatcgaaggc
     1261 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
     1321 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat
     1381 acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
     1441 tcattccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
     1501 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
     1561 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
     1621 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     1681 tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacca
     1741 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     1801 agcggcgggc cggccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     1861 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     1921 atgctccccg ggtaaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     1981 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2041 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2101 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2161 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2221 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     2281 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     2341 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     2401 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     2461 ctgccggctc tgcggcctct tccgcgtgtt cgccttcgcc ctcagacgag tcggatctcc
     2521 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     2581 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgtagac
     2641 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2701 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2761 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2821 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     2881 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     2941 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3001 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     3061 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3121 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3181 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     3241 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     3301 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3361 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3421 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3481 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3541 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3601 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3661 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3721 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3781 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3841 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     3901 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3961 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4021 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4081 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4141 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4201 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4261 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4321 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4381 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4441 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4501 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4561 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4621 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4681 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4741 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4801 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4861 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4921 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4981 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5041 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5101 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5161 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5221 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5281 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5341 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5401 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5461 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5521 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5581 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5641 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5701 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5761 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5821 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5881 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     5941 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6001 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     6061 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     6121 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     6181 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     6241 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     6301 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     6361 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6421 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6481 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     6541 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     6601 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     6661 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     6721 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     6781 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     6841 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     6901 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     6961 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     7021 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     7081 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     7141 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     7201 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     7261 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     7321 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     7381 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     7441 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     7501 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     7561 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     7621 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     7681 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     7741 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     7801 gggaccctgc aggatttaaa tccgagggga cccagagaat tccatagagc ccaccgcatc
     7861 cccagcatgc ctgctattgt ctacccaatc ctcccccttg ctgtcctgcc ccaccccacc
     7921 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
     7981 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
     8041 agatggctgg caactagaag gcacagaatt cgagggccta tttcccatga ttccttcata
     8101 tttgcatata cgatacaagg cggttagaga gataattaga attaatttga ctgtaaacac
     8161 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     8221 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     8281 tttcttggct ttatatatct tgtggaaagg acgaggtacc gctctgcagg tttatagtaa
     8341 gcacctgcct acttttttga aagcgctgat atccttgtca tcgtcatcct tgtaatcgat
     8401 gtcatgatca tatgtatcac cgtcatggtc tttgtagtcc atgttaacgc tagaggatcc
     8461 agcctccgcg gaggttggat cggtcccggt gtcatctatg gaggtcaaaa cagcgtggat
     8521 ggcgtctcca ggcgatctga cggttcacta aacgagctct gcttatatag ggggtacctt
     8581 ctctatcact gatagggagc tcgagacgtg tcgacttctc tatcactgat agggagacgt
     8641 tctagacgta ccttctctat cactgatagg gagctcgaga cgtgtcgact tctctatcac
     8701 tgatagggag acgttctaga cgtaccttct ctatcactga tagggagctc gagacgtgtc
     8761 gacttctcta tcactgatag ggagacgttc tagacgtacc ttctctatca ctgataggga
     8821 gctcgagacg tgtcgacttc tctatcactg atagggagac gtgcggccag cttggatccc
     8881 tcgaggttaa caccggtttc tagatcttga gacaaatggc agtattcatc cacaatttta
     8941 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9001 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9061 attacaggga cagcagagat ccactttggc gccggctcga gatttaaatc ctgcaggcga
     9121 ggggg
//
