LOCUS       sequence-462409-        4583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0724m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP14667 - pAAV-AiE0724m-minBG-iCre-BGHpA (Ali...
FEATURES             Location/Qualifiers
     source          1..4583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             550..570
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1639..1750
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2061..2516
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2148..2167)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2358..2379
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2533..2552)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2652..2674
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2712..2730)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2798..2902
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2903..2971,2972..3763)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3121..3140)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3934..4522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4423..4442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaccggt ccccttacca tttcttcaac ataagggtaa
      181 agcatgtctc caaagtattc cgtagcctcg attggaagct gtgctggcaa attgtcaatg
      241 gaacacatca ggatcccgga gccttcaaca ctaagagagg caacatcctt tattcaaatg
      301 cacaaaatat gacttgaaat ttttcccaga ctctactcca ttccaacatt tgtatcactg
      361 ctattaatat atttatagtc ttgagaaaaa ggcataacac tataaacaag actgcttttc
      421 aaacaaaact ttttttttcc aacttggcag cctatctgag ctcgggctgg gcataaaagt
      481 cagggcagag ccatctattg cttacatttg cttctgggat ccagatcttt cgaagctagc
      541 accatggtgc ccaagaagaa gaggaaagtc tccaacctgc tgactgtgca ccaaaacctg
      601 cctgccctcc ctgtggatgc cacctctgat gaagtcagga agaacctgat ggacatgttc
      661 agggacaggc aggccttctc tgaacacacc tggaagatgc tcctgtctgt gtgcagatcc
      721 tgggctgcct ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc tgaggatgtg
      781 agggactacc tcctgtacct gcaagccaga ggcctggctg tgaagaccat ccaacagcac
      841 ctgggccagc tcaacatgct gcacaggaga tctggcctgc ctcgcccttc tgactccaat
      901 gctgtgtccc tggtgatgag gagaatcaga aaggagaatg tggatgctgg ggagagagcc
      961 aagcaggccc tggcctttga acgcactgac tttgaccaag tcagatccct gatggagaac
     1021 tctgacagat gccaggacat caggaacctg gccttcctgg gcattgccta caacaccctg
     1081 ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac cgatggtggg
     1141 agaatgctga tccacattgg caggaccaag accctggtgt ccacagctgg tgtggagaag
     1201 gccctgtccc tgggggttac caagctggtg gagagatgga tctctgtgtc tggtgtggct
     1261 gatgacccca acaactacct gttctgccgg gtcagaaaga atggtgtggc tgccccttct
     1321 gccacctccc aactgtccac ccgggccctg gaagggatct ttgaggccac ccaccgcctg
     1381 atctatggtg ccaaggatga ctctgggcag agatacctgg cctggtctgg ccactctgcc
     1441 agagtgggtg ctgccaggga catggccagg gctggtgtgt ccatccctga aatcatgcag
     1501 gctggtggct ggaccaatgt gaacattgtg atgaactaca tcagaaacct ggactctgag
     1561 actggggcca tggtgaggct gctcgaggat ggggactaat gaggcgcgcc gaattcaagc
     1621 ttctcgagag atcttcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     1681 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     1741 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     1801 acagcaaggg ggaggattgg gaagacaata gcaggcatga gatctcacgt gcggaccgag
     1861 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     1921 actgaggccg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     1981 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2041 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2101 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
     2161 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2221 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     2281 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     2341 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
     2401 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
     2461 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     2521 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     2581 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     2641 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     2701 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2761 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2821 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2881 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2941 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3001 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3061 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3121 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     3181 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3241 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3301 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3361 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3421 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3481 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3541 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3601 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3661 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3721 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3781 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3841 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3901 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3961 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4021 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4081 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4141 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4201 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4261 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4321 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4381 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4441 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4501 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4561 ttgctggcct tttgctcaca tgt
//
