LOCUS       sequence-462818-        5338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MPS1 from Human codon optimized for S. cerevisiae in pUCIDT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Fabritius A, Alonso A, Wood A, Sulthana S, Winey M
  TITLE     Spindle checkpoint activation by fungal orthologs of the S. 
            cerevisiae Mps1 kinase.
  JOURNAL   PLoS One. 2024 Mar 26;19(3):e0301084. doi: 
            10.1371/journal.pone.0301084. eCollection 2024.
  PUBMED    38530809
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2024-03-26"; volume: "19(3):e0301084. doi"; pages: " 
            10.1371/journal.pone.0301084. eCollection 2024"
COMMENT     Sequence Label: MPS1 Human
FEATURES             Location/Qualifiers
     source          1..5338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     121..140
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     334..356
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     348..365
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     349..365
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3087..3103)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3087..3103)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3100..3122)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3111..3127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3135..3141,3142..3159,3160..3165))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3180..3201
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3318..3335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3489..4077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3569..3588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4248..5039,5040..5108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4871..4890
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5109..5213)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5281..5299
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(join(5337..5338,1..21))
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
       61 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
      121 cggcatcaga gcagattgta ctgagagtgc accaaatgcg gtgtgaaata ccgcacagat
      181 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
      241 aagggcgatc ggtgcgggcc tcatcgctat tacgccagct ggcgaaaggg ggatgtgctg
      301 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
      361 ccagtgcaac gcgatgacga tggatagcga ttcatcgatg agctgacccg atcgccgccg
      421 ccggagggtt gcgtttgaga cgggcgacag atctcgagat ggagtctgag gacctatccg
      481 ggagagaact taccatagac agcatcatga ataaagtaag agacatcaag aacaaattca
      541 aaaacgagga ccttacagat gaattatctt taaacaagat ttccgccgat acgacggata
      601 atagtgggac ggtcaaccag attatgatga tggctaataa cccagaagac tggttgtcat
      661 tgttacttaa gctggaaaag aactccgttc ctctgtctga tgctctactg aacaagctta
      721 tagggcgtta cagtcaagcc atcgaggcat tgccgcccga taagtatggt caaaatgaat
      781 catttgccag gattcaggtg cgtttcgccg aactgaaagc aattcaagag ccagacgacg
      841 cccgtgacta ttttcagatg gccagggcta actgcaagaa attcgcgttt gtccatattt
      901 ctttcgctca atttgaactt tcacaaggca acgtgaaaaa atccaaacag ctattacaga
      961 aagcagtaga gagaggcgca gtgccgttgg agatgctgga gatagctcta aggaatctga
     1021 acctgcaaaa gaaacagcta ttaagcgaag aagagaagaa aaacctgagc gcctctaccg
     1081 tgctgacagc acaggaaagc ttcagcggaa gcttaggaca tctgcagaac cgtaataatt
     1141 cttgtgacag ccgtgggcaa acaacgaagg ctcgttttct atatggggaa aacatgccac
     1201 cccaggatgc agaaatcggc tacaggaata gcttgaggca gacaaacaaa accaagcagt
     1261 catgcccctt tggacgtgtt ccggtcaact tgttaaattc tcccgattgc gacgtgaaga
     1321 cagacgacag tgtagtgccc tgttttatga agcgtcaaac gagtagatct gaatgcagag
     1381 accttgttgt gccagggagc aaaccatcag gtaatgactc ctgcgagctt agaaacctaa
     1441 aaagtgtgca aaactctcac tttaaagaac cactagtaag tgatgagaaa tcttccgagt
     1501 tgataataac ggactccatt acgttgaaaa acaagaccga gagtagcctg ttggcgaagt
     1561 tagaggagac caaggagtac caagaaccgg aagtacctga gtcaaatcaa aaacaatggc
     1621 agagtaaaag aaagtcagag tgtatcaatc agaatccagc cgcctcctcc aaccactggc
     1681 agattcccga attggcgagg aaggttaaca ccgagcagaa acacacaaca ttcgagcagc
     1741 cggtgtttag cgttagtaaa cagtcaccgc ccatatctac gagcaagtgg ttcgatccga
     1801 agtcaatctg taagacgccc agtagtaata cactggatga ttatatgagc tgctttagga
     1861 cccccgtcgt caaaaacgac tttcccccgg catgtcaact ttctaccccg tacggtcaac
     1921 cagcctgctt tcaacagcaa caacaccaaa tcctagccac gcctctgcag aatttacagg
     1981 tcctagctag cagcagcgcc aatgaatgca tctctgtaaa aggacgtatc tatagcatac
     2041 taaagcaaat aggtagcggc ggctcctcta aggtatttca agtactaaac gaaaaaaagc
     2101 aaatttatgc cataaagtac gtaaatttag aagaagccga caatcagact cttgacagct
     2161 atcgtaacga gatcgcttac ttaaataagc tacagcaaca ttcagataaa atcatcagat
     2221 tgtatgatta tgagataacg gaccaataca tatacatggt catggaatgt ggtaacattg
     2281 atctaaatag ttggcttaaa aaaaaaaagt caattgaccc ctgggaacgt aagtcttatt
     2341 ggaaaaatat gttagaggct gtacatacta tacatcagca cggtatagtg cattcagatt
     2401 tgaagccagc taacttttta atcgtcgacg gaatgttgaa actgattgat ttcggaattg
     2461 caaaccaaat gcaaccagat acgacatccg tcgttaagga ctcacaagtg gggacggtta
     2521 attatatgcc acccgaagct ataaaggaca tgtcaagcag ccgtgaaaac gggaagtcca
     2581 agtcaaaaat tagtcctaaa tcagacgtat ggtcccttgg atgcatccta tactacatga
     2641 cttacggtaa gacaccgttt cagcagatta taaaccaaat atcaaaattg catgcaataa
     2701 tcgaccccaa tcacgaaata gagttcccgg acatccctga aaaagactta caggacgtat
     2761 tgaagtgttg cctgaagagg gatccaaagc agaggatatc tatacctgaa ctgctggcgc
     2821 atccctacgt tcaaatccaa acccatcctg tgaatcagat ggcaaaagga accacagaag
     2881 aaatgaaata cgttctaggc caactggttg gcttaaacag tcctaatagt atacttaaag
     2941 ctgccaagac gttgtatgag cactactccg ggggcgagag tcacaactca agcagcagta
     3001 aaacgttcga aaagaagagg gggaaaaaat agtctagaat cagttctgga ccagcgagct
     3061 gtgctgcgac tcgtggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     3121 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     3181 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     3241 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3301 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3601 ctcgtgcgct ctcctgttcc gaccctgtcg cttaccggat acctgtccgc ctttctccct
     3661 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3901 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4261 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4321 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4381 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4441 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4501 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4561 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4621 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4681 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4741 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4801 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4861 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4921 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4981 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5041 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5101 atactcatac tctacctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5161 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5221 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     5281 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacc
//
