LOCUS       sequence-462821-        4693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MPS1 from Rhizoclosmatium globosum codon optimized for S. cerevisiae
            in pUCIDT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Fabritius A, Alonso A, Wood A, Sulthana S, Winey M
  TITLE     Spindle checkpoint activation by fungal orthologs of the S. 
            cerevisiae Mps1 kinase.
  JOURNAL   PLoS One. 2024 Mar 26;19(3):e0301084. doi: 
            10.1371/journal.pone.0301084. eCollection 2024.
  PUBMED    38530809
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2024-03-26"; volume: "19(3):e0301084. doi"; pages: " 
            10.1371/journal.pone.0301084. eCollection 2024"
COMMENT     Sequence Label: MPS1 Rhizoclosmatium globosum
FEATURES             Location/Qualifiers
     source          1..4693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..121
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     315..337
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     329..346
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     330..346
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2423..2439)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2423..2439)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2436..2458)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2447..2463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2471..2477,2478..2495,2496..2501))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2516..2537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2654..2671)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2825..3413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2905..2924)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3584..4375,4376..4444))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4207..4226
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4445..4549)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4617..4635
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(join(4673..4693,1..2))
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
       61 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
      121 actgagagtg caccaaatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
      181 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
      241 ctcatcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      301 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcaa cgcgatgacg
      361 atggatagcg attcatcgat gagctgaccc gatcgccgcc gccggagggt tgcgtttgag
      421 acgggcgaca gatctcgaga tggaccaagc gaagtccata ttgatgagtg gaatcgatgt
      481 tagcgcgcaa cctcttgatg ctttaaaaga cgagttaaac gttcttgatt cccccaagaa
      541 cgatatcgcc cctccccaag caccactgag cgaacccgcg aaatcaacag tagaaccacc
      601 acctacacca caggcagcac tttccacatc cgttgtacca accccacaat cagcgagtgc
      661 aaccagcgcc gcatctttgg catccaagga aaagatgaag aggctacttg cctctttgaa
      721 taggagcatg aataaccgtg aaagaccggc ttgtgtaagt gtacccgaaa gccccgtccc
      781 aatcccaagc gacagcgtca gccagtcaac acctatgcac ggagcgacct ctactcacat
      841 agaggttccc gtccaaaagg aaagagcgca taacacgagc aaggtcgaaa gcccgacagt
      901 cccccttgtt gtagagaaaa gctcacccaa gttgaccgag ccggatagtg cctccaaatc
      961 tgaacagccc agacaagcag ctcccaaagc cgagccaagt aatgacagcc atccgaggcc
     1021 tatcccgaca catcaaccat ttgagcaccc cccgctaccc aggacactgc caacgtcagc
     1081 agcggaatta ccaccagtgc cagcctctca aagtaagatc caatctattt ccagtaagtt
     1141 taggaatact ggccagcctg cccctttgga catcccaaag tcagtagagg aggaatctac
     1201 tccactagcg cctgagggta agcaacctaa gcaacctggt tctttcgaat atcttacacc
     1261 cattaagtcc gaagatgagg aggcagattc ccccgttccc cagcgtgctg gacgtgcctc
     1321 cgacgctatc ccatccaaac cacccgcctt agaaagcaac cataaaggac tgtacatcac
     1381 agtcaataga gtgccatata agagaattga gctgataggt cgtggggcga gttccaaggt
     1441 gtataaagtg attagcgagt ctggtaagat attggcgctg aaaaaagtga aattggacag
     1501 acaggacccc cttgcaattg agggttactt gaacgaaatt cagctgttga aaaaactggt
     1561 caatagcgac gcaatcatta cccttgtcga tagcgagatc tgtgcagaca ggaaaagcct
     1621 gctgctgtta ctagagtacg gcgagattga tcttgctcat ttattgctta aagataagtc
     1681 tctaattggc aacttaaatt tcataaggct acagtggact cagatgttac aggcggtaca
     1741 gcaaatacat aatgaaaaga ttgtccatag cgatttaaaa cccgcaaact ttcttattgt
     1801 ggaaggtaag ctgaagctga tcgatttcgg gattgcaaag tcaataccga gtgatacaac
     1861 aaacattcaa agggattatc aaacaggcac cttgaactac atggcacctg aagccatcat
     1921 gttcacggaa tccaacagca agggtcaaca tttgaagcta gggagagcaa gtgacatctg
     1981 gtccttaggg tgtatcttat accagctggt ctacggtcag ccccctttcg ccgcgctgcc
     2041 aatagtccaa aaactaaatg caatcgtaga tagtaatttc cacattgcct ttggtgaggt
     2101 ctcagactca agtcttctgc aagtaatgaa aagttgcttg aacagggatc cgaaggccag
     2161 gatgacgatt ccggagcttt tagcgcaccc gttcctacat ccccagaccg ctggcgaagg
     2221 cacggtatta gtctccaaga tggatctgct gaaggtccta aaagcaggca tagaattggg
     2281 gaggaggact ggcgatagag gggacgtggg gaaagataca ttggacgcct atgtggaatc
     2341 tatctatgcg gagttagtaa gacgtaaatc tagaatcagt tctggaccag cgagctgtgc
     2401 tgcgactcgt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2461 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2521 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2581 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2641 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2701 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2761 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2821 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2881 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2941 tgcgctctcc tgttccgacc ctgtcgctta ccggatacct gtccgccttt ctcccttcgg
     3001 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3061 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3121 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3181 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3241 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3301 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3361 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3421 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3481 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3541 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3601 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3661 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3721 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3781 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3841 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3901 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3961 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4021 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4081 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4141 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4201 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4261 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4321 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4381 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4441 tcatactcta cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4501 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4561 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     4621 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     4681 acatgcagct ccc
//
