LOCUS       sequence-463055-        2738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Azurite reporter: PT7-tetO-B0035-Azurite-T7 terminator, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Barthel S, Hoffmann-Becking M, Karimov IG, Erb TJ
  TITLE     Genetically encoded control of in vitro transcription-translation 
            coupled DNA replication
  JOURNAL   bioRxiv 2025.07.08.663768; doi: 10.1101/2025.07.08.663768
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.07.08.663768; doi: 10.1101/2025.07.08.663768"
COMMENT     Sequence Label: pTE5430
FEATURES             Location/Qualifiers
     source          1..2738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      722..768
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1187..1565)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     1512..1531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1750..1854)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1957..2545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2446..2465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2672..2691
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2672..2690
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2693..2711
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aatgtctaaa ggtgaagaac tgtttaccgg tgttgttcca attctggttg aactggatgg
       61 tgatgttaac ggtcacaaat tttctgtttc tggtgaaggc gaaggtgatg caacctacgg
      121 taaactgacc ctgaaattta tctgtaccac tggtaaactg ccagttccat ggccaactct
      181 ggttaccact ctgtctcacg gtgttcaatg tttttctcgt tacccagatc acatgaaaca
      241 gcacgatttt ttcaaaagcg caatgccaga aggttacgtt caagaacgta ccatcttctt
      301 caaagatgac ggtaactaca aaacccgtgc ggaagtgaaa tttgaaggtg ataccctggt
      361 taaccgtatc gaactgaaag gtatcgactt caaagaggat ggcaacattc tgggtcacaa
      421 actggaatac aactttaact ctcacaacat ctacatcatg gcggacaaac aaaaaaacgg
      481 catcaaagtg aacttcaaga ttcgccacaa catcgaagat ggttctgttc aactggcaga
      541 tcactaccaa caaaacaccc caattggtga tggtccagtt ctgctgccag ataaccacta
      601 cctgtctacc caatctgcac tgtctaaaga tccaaacgaa aaacgtgatc acatggtgct
      661 gctggaattt cgtaccgcag caggtattac ccacggtatg gatgaactgt acaaagctta
      721 atagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgcg cttgttggag
      781 acgagctgcg gccgcgatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      841 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      901 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      961 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1021 tgataccgcg gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1081 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1141 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1201 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1261 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1321 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1381 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1441 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1501 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1561 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1621 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1681 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1741 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1801 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1861 tccccgaaaa gtgccacctg tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1921 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1981 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2041 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2101 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2161 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2221 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2281 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2341 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2401 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2461 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2521 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2581 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2641 ccgtgcggcc gcaacacgtc tcgagtagga gtaatacgac tcactatagg gatccctatc
     2701 agtgatagag atactagaga ttaaagagga gaataatc
//
