LOCUS       sequence-46315-e        9031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Pratt ZL, Zhang J, Sugden B
  TITLE     The Latent Membrane Protein 1 (LMP1) Oncogene of Epstein-Barr Virus 
            Can Simultaneously Induce and Inhibit Apoptosis in B Cells.
  JOURNAL   J Virol. 2012 Apr;86(8):4380-93. Epub 2012 Feb 8.
  PUBMED    22318153
REFERENCE   2  (bases 1 to 9031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2012-04"; volume: "86(8)"; pages: "4380-93. Epub 2012 Feb 8"
COMMENT     Sequence Label: pCMMP-BiP-IRES-mRFP
FEATURES             Location/Qualifiers
     source          1..9031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        84..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    813..1170
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1235..1651
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1581..1603
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1619..1635
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3889..4454
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3905..4456
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4049..4066)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4276..4295
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4478..5149
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
                     VTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRL
                     KLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTG
                     A"
     CDS             4478..5149
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
                     VTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRL
                     KLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTG
                     A"
     primer_bind     complement(4602..4620)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4870..4889
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5064..5087
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(6412..6428)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6412..6428)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6425..6447)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6436..6452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6460..6466,6467..6484,6485..6490))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6505..6526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6643..6660)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6814..7402)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6894..6913)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7573..8364,8365..8433))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8196..8215
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8434..8538)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8606..8624
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8662..8684)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8784..8803
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8997..9019
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9011..9028
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9012..9028
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tattggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc cttgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggttgt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      601 aaccggcgcc agtcctccga tagactgcgt cgcccgggta cccgtgttct caataaaccc
      661 tcttgcagtt gcatccgact cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
      721 ttgactaccc gtcagcgggg tctttcattt ggaggttcca ccgagatttg gagacccctg
      781 cccagggacc accgaccccc ccgccgggag gtaagctggc cagcaactta tctgtgtctg
      841 tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac
      901 tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc cggccgcaac
      961 cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg agtcctaaaa
     1021 tcccgatcgt ttaggactct ttggtgcacc ccccttagag gagggatatg tggttctggt
     1081 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggga
     1141 ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac
     1201 tgtgtttctg tatttgtctg aaaatatggg cccgggctag actgttacca ctcccttaag
     1261 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1321 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1381 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1441 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1501 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1561 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1621 ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga tcttatatgg
     1681 ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta ctaacagccc
     1741 ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct ggagacctct
     1801 ggcggcagcc taccaagaac aactggaccg accggtcaat tctggcacca tgaagctctc
     1861 cctggtggcc gcgatgctgc tgctgctcag cgcggcgcgg gccgaggagg aggacaagaa
     1921 ggaggacgtg ggcacggtgg tcggcatcga cctggggacc acctactcct gcgtcggcgt
     1981 gttcaagaac ggccgcgtgg agatcatcgc caacgatcag ggcaaccgca tcacgccgtc
     2041 ctatgtcgcc ttcactcctg aaggggaacg tctgattggc gatgccgcca agaaccagct
     2101 cacctccaac cccgagaaca cggtctttga cgccaagcgg ctcatcggcc gcacgtggaa
     2161 tgacccgtct gtgcagcagg acatcaagtt cttgccgttc aaggtggttg aaaagaaaac
     2221 taaaccatac attcaagttg atattggagg tgggcaaaca aagacatttg ctcctgaaga
     2281 aatttctgcc atggttctca ctaaaatgaa agaaaccgct gaggcttatt tgggaaagaa
     2341 ggttacccat gcagttgtta ctgtaccagc ctattttaat gatgcccaac gccaagcaac
     2401 caaagacgct ggaactattg ctggcctaaa tgttatgagg atcatcaacg agcctacggc
     2461 agctgctatt gcttatggcc tggataagag ggagggggag aagaacatcc tggtgtttga
     2521 cctgggtggc ggaaccttcg atgtgtctct tctcaccatt gacaatggtg tcttcgaagt
     2581 tgtggccact aatggagata ctcatctggg tggagaagac tttgaccagc gtgtcatgga
     2641 acacttcatc aaactgtaca aaaagaagac gggcaaagat gtcaggaaag acaatagagc
     2701 tgtgcagaaa ctccggcgcg aggtagaaaa ggccaaacgg gccctgtctt ctcagcatca
     2761 agcaagaatt gaaattgagt ccttctatga aggagaagac ttttctgaga ccctgactcg
     2821 ggccaaattt gaagagctca acatggatct gttccggtct actatgaagc ccgtccagaa
     2881 agtgttggaa gattctgatt tgaagaagtc tgatattgat gaaattgttc ttgttggtgg
     2941 ctcgactcga attccaaaga ttcagcaact ggttaaagag ttcttcaatg gcaaggaacc
     3001 atcccgtggc ataaacccag atgaagctgt agcgtatggt gctgctgtcc aggctggtgt
     3061 gctctctggt gatcaagata caggtgacct ggtactgctt gatgtatgtc cccttacact
     3121 tggtattgaa actgtgggag gtgtcatgac caaactgatt ccaaggaaca cagtggtgcc
     3181 taccaagaag tctcagatct tttctacagc ttctgataat caaccaactg ttacaatcaa
     3241 ggtctatgaa ggtgaaagac ccctgacaaa agacaatcat cttctgggta catttgatct
     3301 gactggaatt cctcctgctc ctcgtggggt cccacagatt gaagtcacct ttgagataga
     3361 tgtgaatggt attcttcgag tgacagctga agacaagggt acagggaaca aaaataagat
     3421 cacaatcacc aatgaccaga atcgcctgac acctgaagaa atcgaaagga tggttaatga
     3481 tgctgagaag tttgctgagg aagacaaaaa gctcaaggag cgcattgata ctagaaatga
     3541 gttggaaagc tatgcctatt ctctaaagaa tcagattgga gataaagaaa agctgggagg
     3601 taaactttcc tctgaagata aggagaccat ggaaaaagct gtagaagaaa agattgaatg
     3661 gctggaaagc caccaagatg ctgacattga agacttcaaa gctaagaaga aggaactgga
     3721 agaaattgtt caaccaatta tcagcaaact ctatggaagt gcaggccctc ccccaactgg
     3781 tgaagaggat acagcagaaa aagatgagtt gtagacactg atctgctagt gctgtaatat
     3841 tgtaaatact ggactcagga acttttgtta ggctcgagga tccggaattc cgcccccccc
     3901 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     3961 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     4021 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     4081 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     4141 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     4201 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     4261 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     4321 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     4381 tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     4441 aaacacgatg ataatatggc cacaaccatg gttcacctcc tccgaggacg tcatcaagga
     4501 gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt tcgagatcga
     4561 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     4621 gggcggcccc ctgcccttcg cctgggacat cctgtcccct cagttccagt acggctccaa
     4681 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     4741 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     4801 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     4861 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcggat
     4921 gtaccccgag gacggcgccc tgaagggcga gatcaagatg aggctgaagc tgaaggacgg
     4981 cggccactac gacgccgagg tcaagaccac ctacatggcc aagaagcccg tgcagctgcc
     5041 cggcgcctac aagaccgaca tcaagctgga catcacctcc cacaacgagg actacaccat
     5101 cgtggaacag tacgagcgcg ccgagggccg ccactccacc ggcgcctaat taattaagaa
     5161 ttccggatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac
     5221 tcaacaatat caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt
     5281 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     5341 ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga gaagttcaga
     5401 tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca
     5461 gttcctgccc cgctcagggc caagaacagt tggaacagga gaatatgggc caaacaggat
     5521 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
     5581 gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     5641 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     5701 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
     5761 tccgatagac tgcgtcgccc gggtacccgt gttctcaata aaccctcttg cagttgcatc
     5821 cgactcgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     5881 cggggtcttt cagtttctcc cacctacaca ggtctcacta acattcctga tgtgccgcag
     5941 ggactccgtc agcccggttt ttgtttataa taaaatgcaa gaacagtgtt cccttcaagc
     6001 cagactacat cctgactctc ggctttataa aagaatgttg aagggctctg tggactatct
     6061 gccacacgac tttttaagat ttttatgcct cctggatgag ggatttagtc aatctatcct
     6121 cgtctatttt gctggcttct ccgtatttta aatttctagt ttgcactccc ttcctgagag
     6181 cacggcgatt gcagagtagt taatactctg agggcaggct tctgtgaaaa ggttgcctgg
     6241 gctcagtgtg agattttgcc ataaaaaggg gtcctgcccc tgtgtacaga cagatcggaa
     6301 tctagagtgc atactcagag tccccgcggt tccggggctc tgatctcagg gcatctttgc
     6361 ctagagatcc tctacgccgg acgcatcgtg gccctcgaat tcgtaatcat ggtcatagct
     6421 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6481 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6541 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6601 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6661 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6721 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6781 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6841 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6901 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6961 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7021 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7081 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7141 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7201 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     7261 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7321 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7381 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7441 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7501 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7561 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7621 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7681 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7741 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7801 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7861 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7921 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7981 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8041 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8101 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8161 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8221 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8281 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8341 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8401 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8461 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8521 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     8581 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     8641 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     8701 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     8761 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     8821 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
     8881 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     8941 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     9001 gtcacgacgt tgtaaaacga cggccagtgc c
//
