LOCUS       sequence-46360-e        9528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Zhu Y, Xu J, Heinemann SF
  TITLE     Two pathways of synaptic vesicle retrieval revealed by 
            single-vesicle imaging.
  JOURNAL   Neuron. 2009 Feb 12;61(3):397-411.
  PUBMED    19217377
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2009-02-12"; volume: "61(3)"; pages: "397-411"
COMMENT     Sequence Label: pcDNA3-SypHluorin 4x (S4x)
FEATURES             Location/Qualifiers
     source          1..9528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      936..945
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1494..1510
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1494..1510
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      1524..1533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1530..2246
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Miesenbock et al., 1998)"
                     /label=ecliptic pHluorin
                     /note="responds to a drop in pH with a gradual loss 
                     (eclipsing) of excitation at 475 nm, accompanied by a 
                     reduction of excitation at 395 nm"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     regulatory      2286..2295
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2292..3008
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Miesenbock et al., 1998)"
                     /label=ecliptic pHluorin
                     /note="responds to a drop in pH with a gradual loss 
                     (eclipsing) of excitation at 475 nm, accompanied by a 
                     reduction of excitation at 395 nm"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     regulatory      3051..3060
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3057..3773
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Miesenbock et al., 1998)"
                     /label=ecliptic pHluorin
                     /note="responds to a drop in pH with a gradual loss 
                     (eclipsing) of excitation at 475 nm, accompanied by a 
                     reduction of excitation at 395 nm"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     regulatory      3813..3822
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3819..4535
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Miesenbock et al., 1998)"
                     /label=ecliptic pHluorin
                     /note="responds to a drop in pH with a gradual loss 
                     (eclipsing) of excitation at 475 nm, accompanied by a 
                     reduction of excitation at 395 nm"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             4971..4997
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5012..5041
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5057..5074
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5118..5135)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5124..5348
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5394..5822
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5481..5500)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5691..5712
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5831..5851)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5836..6166
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6017..6152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6079..6098
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6233..7027
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6287..6306)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6897..6916
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7201..7322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7238..7257)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7292..7311
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7371..7387)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7371..7387)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7384..7406)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7395..7411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7419..7425,7426..7443,7444..7449))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7464..7485
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7602..7619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7773..8361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7853..7872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8532..9323,9324..9392))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9155..9174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9393..9497)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagctca agcttgccac catggacgtg gtgaatcagc
      961 tggtggctgg gggtcagttc cgggtggtca aggagcccct tggcttcgtg aaggtgctgc
     1021 agtgggtctt tgccatcttc gcctttgcta cgtgcggcag ctacaccgga gagcttcggc
     1081 tgagcgtgga gtgtgccaac aagacggaga gtgccctcaa catcgaagtc gaatttgagt
     1141 acccattcag gctgcaccaa gtgtactttg atgcaccctc ctgcgttaaa gggggcacta
     1201 ccaagatctt cctagttggt gactactcct cctcgggtga attctttgtc accgtggctg
     1261 tgtttgcctt cctctactcc atgggggccc tggccaccta catcttcctg cagaacaagt
     1321 accgagagaa caacaaaggg ccaatgatgg acttcctggc cacagcagtg ttcgctttca
     1381 tgtggctagt tagctcatcc gcctgggcca aaggcctgtc cgatgtgaag atggccactg
     1441 acccagagaa cattatcaag gagatgccta tgtgccgcca gacagatggc cgctctagaa
     1501 ctagtggatc ccccatggat ctagccacca tgggaagtaa aggagaagaa cttttcactg
     1561 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
     1621 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     1681 ctggaaaact acctgttcca tggccaacac ttgtcactac tttctcttat ggtgttcaat
     1741 gcttttcaag atacccagat catatgaaac ggcatgactt tttcaagagt gccatgcccg
     1801 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
     1861 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
     1921 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac gatcaccagg
     1981 tgtacatcat ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa attagacaca
     2041 acattgaaga tggaggcgtt caactagcag accattatca acaaaatact ccaattggcg
     2101 atgggcccgt ccttttacca gacaaccatt acctgtttac aacttctact ctttcgaaag
     2161 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
     2221 cacatggcat ggatgaacta tacaaaaccg gtggagctcg aatctgcaga attcgccctt
     2281 ggctagccac catgggaagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     2341 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     2401 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     2461 catggccaac acttgtcact actttctctt atggtgttca atgcttttca agatacccag
     2521 atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
     2581 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
     2641 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     2701 ttcttggaca caaattggaa tacaactata acgatcacca ggtgtacatc atggcagaca
     2761 aacaaaagaa tggaatcaaa gctaacttca aaattagaca caacattgaa gatggaggcg
     2821 ttcaactagc agaccattat caacaaaata ctccaattgg cgatgggccc gtccttttac
     2881 cagacaacca ttacctgttt acaacttcta ctctttcgaa agatcccaac gaaaagagag
     2941 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     3001 tatacaaaac cggtggagct cgagggctgc aggaattcga tatggatcta gccaccatgg
     3061 gaagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     3121 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
     3181 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
     3241 tcactacttt ctcttatggt gttcaatgct tttcaagata cccagatcat atgaaacggc
     3301 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     3361 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
     3421 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     3481 tggaatacaa ctataacgat caccaggtgt acatcatggc agacaaacaa aagaatggaa
     3541 tcaaagctaa cttcaaaatt agacacaaca ttgaagatgg aggcgttcaa ctagcagacc
     3601 attatcaaca aaatactcca attggcgatg ggcccgtcct tttaccagac aaccattacc
     3661 tgtttacaac ttctactctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     3721 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaaaccggtg
     3781 gagctcgaat ctgcagaatt cgcccttggc tagccaccat gggaagtaaa ggagaagaac
     3841 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     3901 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     3961 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttctcttatg
     4021 gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg
     4081 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     4141 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     4201 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataacg
     4261 atcaccaggt gtacatcatg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa
     4321 ttagacacaa cattgaagat ggaggcgttc aactagcaga ccattatcaa caaaatactc
     4381 caattggcga tgggcccgtc cttttaccag acaaccatta cctgtttaca acttctactc
     4441 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     4501 ctgggattac acatggcatg gatgaactat acaaaaccgg tggagctcga tcaagcttat
     4561 cgatcacatg caaggaactg agggaccctg tgacttcagg actcaacacc tcggtggtgt
     4621 ttggcttcct gaacctggtg ctctgggttg gcaacctatg gttcgtgttc aaggagacag
     4681 gctgggccgc cccattcatg cgcgcacctc caggcgcccc agaaaagcaa ccagctcctg
     4741 gcgatgccta cggcgatgcg ggctatgggc agggccccgg aggctatggg ccccaggact
     4801 cctacgggcc tcagggtggt tatcaacccg attacgggca gccagccagc ggcggtggcg
     4861 gtggctacgg gcctcagggc gactatgggc agcaaggcta cggccaacag ggtgcgccca
     4921 cctccttctc caatcagatg tcggatccga agcttcggta ccaagctgcc tacccttacg
     4981 atgtgcccga ttacgcctga tagcttacgt agaacaaaaa ctcatctcag aagaggatct
     5041 gaatagcgcc gtcgaccatc atcatcatca tcattgagtt taaacggtct ccagcttaag
     5101 tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5161 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5221 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5281 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5341 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     5401 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5461 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5521 cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt
     5581 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5641 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5701 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5761 tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5821 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg
     5881 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5941 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6001 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6061 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     6121 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     6181 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     6241 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6301 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6361 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     6421 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6481 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6541 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6601 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6661 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     6721 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     6781 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6841 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6901 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6961 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     7021 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     7081 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     7141 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     7201 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7261 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7321 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7381 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7441 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7501 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7561 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7621 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7681 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7741 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7801 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7861 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7921 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     7981 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8041 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8101 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8161 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     8221 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8281 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8341 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8401 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8461 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8521 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8581 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8641 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8701 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8761 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8821 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8881 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8941 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9001 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9061 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9121 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9181 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9241 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9301 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9361 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9421 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9481 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
