LOCUS       sequence-464291-        5968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient expresses IRF3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Chou WC, Chen WT, Shen CY
  TITLE     A common variant in 11q23.3 associated with hyperlipidemia is 
            mediated by the binding and regulation of GATA4.
  JOURNAL   NPJ Genom Med. 2022 Jan 19;7(1):4. doi: 10.1038/s41525-021-00279-5.
  PUBMED    35046404
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "NPJ Genom 
            Med."; date: "2022-01-19"; volume: "7(1):4. doi"; pages: " 
            10.1038/s41525-021-00279-5"
COMMENT     Sequence Label: pXJ-Myc-IRF3
FEATURES             Location/Qualifiers
     source          1..5968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          670..1242
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1250..1269
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1297..1315
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1336..1365
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(2767..2785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3059..3180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3096..3115)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3150..3169
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3379..3967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3459..3478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4138..4929,4930..4998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4761..4780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4999..5103)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5171..5189
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5227..5249)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5349..5368
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5435..5890)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5572..5593)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5784..5803
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg
      601 ggaacggtgc attggaacgg acccctaggc ttttgcaaaa agctccggat cgatcctgag
      661 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      721 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      781 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      841 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      901 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      961 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
     1021 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
     1081 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
     1141 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
     1201 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1261 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
     1321 ttcaccatgg cagaggagca aaagctcatt tctgaagagg acttgttgcg gaaacatatg
     1381 ggatcccccg ggctgcagga attcgatatc aagcttacca tgggaacccc aaagccacgg
     1441 atcctgccct ggctggtgtc gcagctggac ctggggcaac tggagggcgt ggcctgggtg
     1501 aacaagagcc gcacgcgctt ccgcatccct tggaagcacg gcctacggca ggatgcacag
     1561 caggaggatt tcggaatctt ccaggcctgg gccgaggcca ctggtgcata tgttcccggg
     1621 agggataagc cagacctgcc aacctggaag aggaatttcc gctctgccct caaccgcaaa
     1681 gaagggttgc gtttagcaga ggaccggagc aaggaccctc acgacccaca taaaatctac
     1741 gagtttgtga actcaggagt tggggacttt tcccagccag acacctctcc ggacaccaat
     1801 ggtggaggca gtacttctga tacccaggaa gacattctgg atgagttact gggtaacatg
     1861 gtgttggccc cactcccaga tccgggaccc ccaagcctgg ctgtagcccc tgagccctgc
     1921 cctcagcccc tgcggagccc cagcttggac aatcccactc ccttcccaaa cctggggccc
     1981 tctgagaacc cactgaagcg gctgttggtg ccgggggaag agtgggagtt cgaggtgaca
     2041 gccttctacc ggggccgcca agtcttccag cagaccatct cctgcccgga gggcctgcgg
     2101 ctggtggggt ccgaagtggg agacaggacg ctgcctggat ggccagtcac actgccagac
     2161 cctggcatgt ccctgacaga caggggagtg atgagctacg tgaggcatgt gctgagctgc
     2221 ctgggtgggg gactggctct ctggcgggcc gggcagtggc tctgggccca gcggctgggg
     2281 cactgccaca catactgggc agtgagcgag gagctgctcc ccaacagcgg gcatgggcct
     2341 gatggcgagg tccccaagga caaggaagga ggcgtgtttg acctggggcc cttcattgta
     2401 gatctgatta ccttcacgga aggaagcgga cgctcaccac gctatgccct ctggttctgt
     2461 gtgggggagt catggcccca ggaccagccg tggaccaaga ggctcgtgat ggtcaaggtt
     2521 gtgcccacgt gcctcagggc cttggtagaa atggcccggg tagggggtgc ctcctccctg
     2581 gagaatactg tggacctgca catttccaac agccacccac tctccctcac ctccgaccag
     2641 tacaaggcct acctgcagga cttggtggag ggcatggatt tccagggccc tggggagagc
     2701 tgagccctcg agggggggcc cggtacctta attaattaag gtaccaggta agtgtaccca
     2761 attcgcccta tagtgagtcg tattacaatt cactcgatcg cccttcccaa cagttgcgca
     2821 gcctgaatgg cgaatggaga tccaattttt aagtgtataa tgtgttaaac tactgattct
     2881 aattgtttgt gtattttaga ttcacagtcc caaggctcat ttcaggcccc tcagtcctca
     2941 cagtctgttc atgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     3001 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3061 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3121 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3181 acgcgcgact ctagactctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3241 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3301 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3361 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3781 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4441 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4501 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4561 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4621 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4681 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4741 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4801 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4861 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4921 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4981 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5041 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5101 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     5161 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     5221 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     5281 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     5341 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     5401 gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta
     5461 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     5521 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     5581 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     5641 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     5701 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     5761 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     5821 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     5881 cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     5941 cctcttcgct attacgccag ctagagtc
//
