LOCUS       sequence-464304-        9655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses HAVCR1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9655)
  TITLE     Shen Lab plasmids
REFERENCE   2  (bases 1 to 9655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLAS2w.Phyg-HAVCR1
FEATURES             Location/Qualifiers
     source          1..9655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        295..598
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        599..802
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     753..773
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        847..865
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1997..2020
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2068..2185
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2234..2744
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2663..2681
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2756..3775
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    3806..4394
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3859..3879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4277..4288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4466..4699
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4777..4898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4814..4833)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4868..4887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4938..5073
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5094..5112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5122..5139)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5122..5138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5131..5153)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5280..5735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5367..5386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5577..5598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5761..5865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5866..5934,5935..6726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6084..6103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6897..7485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7386..7405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7639..7656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7773..7794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7809..7814,7815..7832,7833..7839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7847..7863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7852..7874
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7871..7887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7871..7887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7906..7926
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7908..7926
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7954..8180
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8181..8361
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8408..8533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9026..9259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9444..9488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
       61 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc gaggatccac
      121 tagtgaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
      181 gggccagaaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttctagtta
      241 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      301 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      361 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      421 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      481 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      541 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      601 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      661 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      721 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      781 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
      841 cgaaattaat acgactcact atagggagac ccaagctggc tagcagatat ccatcacact
      901 ggcggccgcc accatgcatc ctcaagtggt catcttaagc ctcatcctac atctggcaga
      961 ttctgtagct ggttctgtaa aggttggtgg agaggcaggt ccatctgtca cactaccctg
     1021 ccactacagt ggagctgtca catcaatgtg ctggaataga ggctcatgtt ctctattcac
     1081 atgccaaaat ggcattgtct ggaccaatgg aacccacgtc acctatcgga aggacacacg
     1141 ctataagcta ttgggggacc tttcaagaag ggatgtctct ttgaccatag aaaatacagc
     1201 tgtgtctgac agtggcgtat attgttgccg tgttgagcac cgtgggtggt tcaatgacat
     1261 gaaaatcacc gtatcattgg agattgtgcc acccaaggtc acgactactc caattgtcac
     1321 aactgttcca accgtcacga ctgttcgaac gagcaccact gttccaacga caacgactgt
     1381 tccaacgaca actgttccaa caacaatgag cattccaacg acaacgactg ttccgacgac
     1441 aatgactgtt tcaacgacaa cgagcgttcc aacgacaacg agcattccaa caacaacaag
     1501 tgttccagtg acaacaacgg tctctacctt tgttcctcca atgcctttgc ccaggcagaa
     1561 ccatgaacca gtagccactt caccatcttc acctcagcca gcagaaaccc accctacgac
     1621 actgcaggga gcaataagga gagaacccac cagctcacca ttgtactctt acacaacaga
     1681 tgggaatgac accgtgacag agtcttcaga tggcctttgg aataacaatc aaactcaact
     1741 gttcctagaa catagtctac tgacggccaa taccactaaa ggaatctatg ctggagtctg
     1801 tatttctgtc ttggtgcttc ttgctctttt gggtgtcatc attgccaaaa agtatttctt
     1861 caaaaaggag gttcaacaac taagtgtttc atttagcagc cttcaaatta aagctttgca
     1921 aaatgcagtt gaaaaggaag tccaagcaga agacaatatc tacattgaga atagtcttta
     1981 tgccacggac ctcgaggatt acaaggatga cgacgataag taggccgaat tctcgacctc
     2041 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2101 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2161 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     2221 gccgcggctc gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
     2281 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
     2341 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
     2401 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
     2461 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
     2521 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
     2581 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
     2641 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
     2701 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccatgaa
     2761 aaagcctgaa ctcaccgcta cctctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     2821 ctccgacctg atgcagctct ccgagggcga agaatctcgg gctttcagct tcgatgtggg
     2881 agggcgtgga tatgtcctgc gggtgaatag ctgcgccgat ggtttctaca aagatcgcta
     2941 tgtttatcgg cactttgcat ccgccgctct acctattccc gaagtgcttg acattgggga
     3001 gttcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca ccttgcaaga
     3061 cctgcctgaa accgaactgc ccgctgttct ccagcccgtc gccgaggcca tggatgccat
     3121 cgctgccgcc gatcttagcc agaccagcgg gttcggccca ttcggacctc aaggaatcgg
     3181 tcaatacact acatggcgcg atttcatctg cgctattgct gatccccatg tgtatcactg
     3241 gcaaactgtg atggacgaca ccgtcagtgc ctccgtcgcc caggctctcg atgagctgat
     3301 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgccgatt tcggctccaa
     3361 caatgtcctg accgacaatg gccgcataac agccgtcatt gactggagcg aggccatgtt
     3421 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccctggt tggcttgtat
     3481 ggagcagcag acccgctact tcgagcggag gcatcccgag cttgcaggat ctcctcggct
     3541 ccgggcttat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     3601 tttcgatgat gcagcttggg ctcagggtcg ctgcgacgca atcgtccggt ccggagccgg
     3661 gactgtcggg cgtacacaaa tcgcccgcag aagcgctgcc gtctggaccg atggctgtgt
     3721 ggaagtgctc gccgatagtg gaaacagacg ccccagcact cgtcctaggg caaaggatct
     3781 gcagtaatga acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     3841 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3901 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3961 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4021 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4081 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4141 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4201 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4261 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4321 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4381 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     4441 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     4501 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4561 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     4621 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4681 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     4741 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     4801 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4861 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     4921 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4981 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5041 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     5101 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     5161 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     5221 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     5281 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5341 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5401 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5461 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     5521 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5581 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     5641 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5701 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     5761 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5821 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5881 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5941 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6001 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6061 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6121 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6181 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6241 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6301 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6361 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6421 acaacgttgc gaaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6481 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6541 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6601 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6661 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6721 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6781 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6841 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6901 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6961 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7021 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7081 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7141 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7201 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7261 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7321 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7381 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7441 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7501 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7561 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7621 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7681 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7741 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7801 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7861 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     7921 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     7981 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     8041 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     8101 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     8161 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     8221 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8281 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8341 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     8401 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     8461 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     8521 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     8581 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     8641 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     8701 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8761 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     8821 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     8881 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     8941 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     9001 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     9061 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     9121 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     9181 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     9241 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     9301 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     9361 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     9421 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     9481 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     9541 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     9601 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgag
//
