LOCUS       sequence-464660-        6254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_repABC-p42b_PartType3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK028 [Origin_repABC-p42b_PartType3]
FEATURES             Location/Qualifiers
     source          1..6254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5232..5323
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5324..5392,5393..6184)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      6188..6227
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccctt tcagttacga tagagttcca cgagaagtta gtctttctga
      661 agattgccga tcccgatatt cgtgaaacga tcatcgcatt ggtaaaagcg atggcgcagg
      721 cggaaagcgg tccggctttg ccttcgatag cgaatatcac ccgtccgcct ctggagtagc
      781 tggccaggcg gcaattcccc tgtgaactca ggctcgaatg atctggcaag ttacggctga
      841 tcgaccagac ccggcacctt accgccgcgc cccgctccat ccacgccagc gaaccgatgc
      901 cttgcatctg cgggcttgca gcaaggcgct ttgcacgccc gacattgcga ttcggattat
      961 actgctgctg ttcccggccg ggctcgtccg attcggcctg tgaatgtgcg attgctggac
     1021 ccgccagcgg cacgcccggc cggcgcaagc gcttgaaata gactggacaa ccttttgata
     1081 ggatcttgtt aaccctattt gccttgccac ccctccagaa tcggcgctaa atgcggtttc
     1141 aaagcgcgta gagcttttaa atcgcatttt cgagatttcc atgaatatgg caccgcaaat
     1201 agaaaaagca atttccgatg tcgaccagct gatcatcggt caggcacagg aactatccga
     1261 caagctgaag cagcatcgcc tcgaaatgtt tcctccgcgc gcgctaaagg gtctgcggga
     1321 atttcagctt gctgaagtgg cgcggtttct cggcgtgacc agcggctatc ttcgaaatct
     1381 ttcgctcgag ggcaagggtg ccctccccca ggttacgccg tctggccgcc gatcgtatac
     1441 ggcggagcag atggaggaga tgcgtggctt tctcgagcat aatgcgcgcg ccggaacaca
     1501 ttatgtgcgt catcgccgcg gccacgaaca tctgcaggtc gttgctgtcg tcaacttcaa
     1561 ggggggcagc ggcaagacga cgagtgccgc gcatcttgcc cagcatctcg ccctgaccgg
     1621 tcaccgcgtc ctggccgtcg atctcgatcc gcaagcgtct ctttctgcca tacacggctt
     1681 tcagccggag ttcgacgtca acgaaaacga gacactctac gccgccattc gctatgacga
     1741 tcagcggcgt ccgctgcggg acattatccg gccgaccaac ttcccgaacc ttcatctcgt
     1801 gccgggcaat ctcgaactga tggaattcga gcacgatacg ccgcgggtcc tcgctcaggg
     1861 caaagcgagc gattatgggc gcgtcttctt tgcccggctg gatgaggcgc tggcctcggt
     1921 cgcggatgat tacgacgtcg tcatcatcga ttgccctccc cagcttggct ttttgaccat
     1981 gagcgccatt tgcggtgcga cggcggttct gatcaccgtt catccgcaga tgctcgatgt
     2041 catgtcgatg tgccagttcc tgcaaatgct tggcgaagtg ctgaacacgc tgaagggagc
     2101 cggcggcaat atgaacctcg actggctgcg ttacctcgtg acccgctacg atccgcagga
     2161 cgggccgcag acgcagatgg tcgccttcat gcgctcgatg ttcaagagcc atgtgctcac
     2221 caacccgatg ctgcgcagcg tcgcgatctc cgatgcggcg atgactaacc agacgctgta
     2281 cgaagtcgag cgaagtcagt ttacccgcgc gacctatgac cgcgccatgg aggcgatgga
     2341 gtcagtcaac acggaaatcg tcgatctcat ccataaggca tggggccgga aatgagcggg
     2401 aagtttactg cggtaaaccg gggcaaggag ctggttgatg gcgcgtaaaa atcttctgtc
     2461 gggcctgatg gacgattcca agaagtttac tgcggtaaac aatgaggatg agccggcccc
     2521 tcgcgacgag aggcagcata tcacctataa ggggattggc gcgctcggtg cggtcacccg
     2581 cagcatcgat gccttggccg ccaaggccga tgccgccaag gcgatcgaag agcagctggc
     2641 gagcggcgag acggtgatcg atctcgaccc ggcgctgatc gaagattcct tcgtgaccga
     2701 ccggctggcg catactgacg aacagttccg ggaattagtc gaggcgatcc gcctgcgcgg
     2761 ccaggattca ccgatccttg tgcgcccgca tcccgagagg gaggggcgat atcagatcgc
     2821 ttttggtcat cgtcgtgcgc gggcggcaaa ggagctcggc cgacctgtcc gggcggtggt
     2881 caagagactc gacgaccgcg accatgtcat tgcgcagggc caggagaatt cggcacgcgc
     2941 cgatctctcc ttcatcgaga ggacgatgtt tgccgacaag ctcgacacgt tgggtttcga
     3001 ccgggaaacg atcatgtcgg cactcagcgc agacaagacg acggtgtcca agatgctgtc
     3061 ggtcacgaag cggattccgg ccgaagtcct ggccgcgatc ggcgtagcca agacgactgg
     3121 ccgcgatcgc tggcatgatc tttcggcgaa attcgagacg gaaaacatcg cagcccgggc
     3181 gattgagttc accagatcgg tggagttcga gacggccgag ccggacgccc gcttcgacat
     3241 gcttgtcgcc ttcatcagca ggaaccaaca ggcgtcgccc gccgccgcag ttcagcccgt
     3301 ggcgcacgcc tggcagcgcc gggacggagc cgtcaaagcg aagatcaagg atgatggaaa
     3361 acagttcacc atcgcgctga aggcggagaa ggcatctgcc tttggcgcct acatcgccag
     3421 caatctggat cgtctctacg aggcgttcga gaagacacag gatttgacga agaacggaga
     3481 tcaataagca aaagaaaagg cccccgaacg ttgccgtcgc ggaagccctc tctgatctag
     3541 acacccagag aatcacattt ccgcgaatca tagtcaagag tctttggcac cgaatttggt
     3601 gaggcgtgat cttttgcctt gaagaaggcg aagaaaatgg aaagcggaag tgtgacgacg
     3661 cccttcgggc ggcggccgat gacgtttggc atgttggcaa gtcaggccgc tgcccgaaaa
     3721 atcgagcccg gccgatcgat tgataaatgg aaactctatc gcgccctgtg cgaggccagg
     3781 ccgctgctcg gcattacaga ccgggcgctt tccatcctga acgccttgct gagcttttat
     3841 cccaagggag agctctccga ggagaacggc ttggtcgtat ttccttcgaa tacgcagctc
     3901 tctctgcgcg cgcatggaat ggcggagcag acgatccgtc gccacctggc ggcgctgatc
     3961 gaggccggat tgctgatccg caaggacagc ccgaacggca agcgttacgc tcgcaaggag
     4021 cagggcgggg agatccgtga ggcattcggc ttctcgctgg cgccgctgct tgcgcgcgcg
     4081 gacgagatcg agcggctggc ggccgagatg gcagccgaac gattgcagct gcagcggctc
     4141 aaagagcgcc tgacgctttg caggcgcgac atcgccaagc tcatcgagat ggccgtggaa
     4201 gagggcgcct ctggtgattg gagcggggtc cacctgcatt ttcgaggcat cgtcgaacgg
     4261 ctgccgcgct cgccatcctt cgagaagatt gccgccgcac tcgacgaact ggaactgttg
     4321 cgcgaagaaa tcaccaatca gttggaaatg caggttaaag ctccaaatca aagcggcaat
     4381 gcccaacatt ctgagcggca catacagaat tcaaacccac actacactac tgaacttgaa
     4441 ccacgcttcg aaacgaagca gggcgcaacg gtggacgacc aatcggaagg ttgggccgag
     4501 ccgagggcca gaatagcgac acaggagggg aagggcaggc ccatgccagc tgcaaactca
     4561 gcctcggttg ccggcggcgg gctcaaatcc ttcccgctgg gcctggtgct gcaggcctgc
     4621 ccagagatcg ccgcctatgg accgcagggc tcggtgggca catggcgcga tctgatggcg
     4681 gcggctgtcg tcgtgcgctc aatgctcgga gtgagcccct cggcctatga acaggcctgc
     4741 gaggtcatgg ggcctgaaaa tgcggctacc gtcatagcgt gcgtgcttga aagggcaggg
     4801 cacatcaatt ccgccggtgg ttacctccgt gacctgaccc gccgcgccga gagaggcgaa
     4861 tttgccatcg gaccgatgtt gatggcgctt gtccgcacca attcgccaat cagaggcaag
     4921 accggatgaa aaatttcgtg tcatctgtcc gtggcgatct atggttaacg aaaggtgacg
     4981 gaaataaaca gggtgataac ttcttgttag gatattcttc ctttgagatc atgaagttac
     5041 aagatttttg attggcaagg cgagggaagg acctgatcga cgatcggccg gcgttcgctg
     5101 gagaaacggt gcggttggag ttgacgtgaa aagccttttt gacgactgga ttgtatcaac
     5161 ggaccagtga cagtttctgg ctgagaccag agtagcccgc ctaatgagcg ggcttttttt
     5221 taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5281 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagca ttcagcattt
     5341 tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg ccggtgtttg cgcatccgga
     5401 aaccctggtg aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg gctatattga
     5461 actggatctg aacagcggca aaattctgga atcttttcgt ccggaagaac gttttccgat
     5521 gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg atgcgggcca
     5581 ggaacaactg ggccgtcgta ttcattatag ccagaacgat ctggtggaat atagcccggt
     5641 gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg cggcgattac
     5701 catgagcgat aacaccgcgg cgaacctgct gctgacgacc attggcggtc cgaaagaact
     5761 gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt gggaaccgga
     5821 actgaacgaa gcgattccga acgatgaacg tgataccacc atgccggcag caatggcgac
     5881 caccctgcgt aaactgctga cgggtgagct gctgaccctg gcaagccgcc agcaactgat
     5941 tgattggatg gaagcggata aagtggcggg tccgctgctg cgtagcgcgc tgccggctgg
     6001 ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc agccgtggca ttattgcggc
     6061 gctgggcccg gatggtaaac cgagccgtat tgtggtgatt tataccaccg gcagccaggc
     6121 gacgatggat gaacgtaacc gtcagattgc ggaaattggc gcgagcctga ttaaacattg
     6181 gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga cccttgtcag
     6241 atcaaaggat cttc
//
