LOCUS       sequence-46470-e         562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su 
            Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, 
            Flotte TR, Zamore PD, Gao G
  TITLE     Long-term, efficient inhibition of microRNA function in mice using 
            rAAV vectors.
  JOURNAL   Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.
  PUBMED    22388288
REFERENCE   2  (bases 1 to 562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-4- 4"; volume: "9"; 
            issue: "4"; pages: "403-9"
COMMENT     Sequence Label: pAAV CB6 FFLuc
FEATURES             Location/Qualifiers
     source          1..562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(171..190)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    236..291
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   384..513
                     /label=AAV2 ITR
     primer_bind     complement(532..549)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(532..548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
       61 atcctcataa aggccaagaa gggcggaaag atcgccgtgt aattctagat tccgagatat
      121 cggtaatgct ctagagtcga ggacggggtg aactacgcct gaggatccga tctttttccc
      181 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
      241 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagc
      301 aattcgttga tctgaatttc gaccacccat aatacccatt accctggtag ataagtagca
      361 tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct
      421 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
      481 ccgggcggcc tcagtgagcg agcgagcgcg cagccttaat taacctaatt cactggccgt
      541 cgttttacaa cgtcgtgact gg
//
