LOCUS       sequence-464703-        2413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            CDS_EBFP2_PartType6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK077 [CDS_EBFP2_PartType6]
FEATURES             Location/Qualifiers
     source          1..2413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(617..619,620..622,623..1336)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(662..683)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        1391..1482
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1483..1551,1552..2343)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2347..2386
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctcttatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      661 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgaggg gcgagggcga
      721 gggcgatgcc accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      781 cgtgccctgg cccaccctcg tgaccaccct gagccacggc gtgcagtgct tcgcccgcta
      841 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      901 ggagcgcacc atcttcttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt
      961 cgagggcgac accctggtga accgcatcga gctgaagggc gtcgacttca aggaggacgg
     1021 caacatcctg gggcacaagc tggagtacaa cttcaacagc cacaacatct atatcatggc
     1081 cgtcaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaacg tggaggacgg
     1141 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1201 gctgcccgac agccactacc tgagcaccca gtccgtgctg agcaaagacc ccaacgagaa
     1261 gcgcgatcac atggtcctgc tggagttccg caccgccgcc gggatcactc tcggcatgga
     1321 cgagctgtac aagtaaatcc tgagaccaga gtagcccgcc taatgagcgg gctttttttt
     1381 aattccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1441 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagcat tcagcatttt
     1501 cgtgtggcgc tgattccgtt ttttgcggcg ttttgcctgc cggtgtttgc gcatccggaa
     1561 accctggtga aagtgaaaga tgcggaagat caactgggtg cgcgcgtggg ctatattgaa
     1621 ctggatctga acagcggcaa aattctggaa tcttttcgtc cggaagaacg ttttccgatg
     1681 atgagcacct ttaaagtgct gctgtgcggt gcggttctga gccgtgtgga tgcgggccag
     1741 gaacaactgg gccgtcgtat tcattatagc cagaacgatc tggtggaata tagcccggtg
     1801 accgaaaaac atctgaccga tggcatgacc gtgcgtgaac tgtgcagcgc ggcgattacc
     1861 atgagcgata acaccgcggc gaacctgctg ctgacgacca ttggcggtcc gaaagaactg
     1921 accgcgtttc tgcataacat gggcgatcat gtgacccgtc tggatcgttg ggaaccggaa
     1981 ctgaacgaag cgattccgaa cgatgaacgt gataccacca tgccggcagc aatggcgacc
     2041 accctgcgta aactgctgac gggtgagctg ctgaccctgg caagccgcca gcaactgatt
     2101 gattggatgg aagcggataa agtggcgggt ccgctgctgc gtagcgcgct gccggctggc
     2161 tggtttattg cggataaaag cggtgcgggc gaacgtggca gccgtggcat tattgcggcg
     2221 ctgggcccgg atggtaaacc gagccgtatt gtggtgattt ataccaccgg cagccaggcg
     2281 acgatggatg aacgtaaccg tcagattgcg gaaattggcg cgagcctgat taaacattgg
     2341 taaaccgata caattaaagg ctccttttgg agcctttttt tttggacgac ccttgtcaga
     2401 tcaaaggatc ttc
//
