LOCUS       sequence-464721-        1866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Term_bla-v1_PartType7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK095 [Term_bla-v1_PartType7]
FEATURES             Location/Qualifiers
     source          1..1866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        844..935
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(936..1004,1005..1796)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1800..1839
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctatccc tgtcagacca agtttactca tatatacttt agattgattt
      661 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      721 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      781 aggatcttcg ctgtgagacc agagtagccc gcctaatgag cgggcttttt tttaattccc
      841 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      901 gataaatgct tcaataatat tgaaaaagga agagtatgag cattcagcat tttcgtgtgg
      961 cgctgattcc gttttttgcg gcgttttgcc tgccggtgtt tgcgcatccg gaaaccctgg
     1021 tgaaagtgaa agatgcggaa gatcaactgg gtgcgcgcgt gggctatatt gaactggatc
     1081 tgaacagcgg caaaattctg gaatcttttc gtccggaaga acgttttccg atgatgagca
     1141 cctttaaagt gctgctgtgc ggtgcggttc tgagccgtgt ggatgcgggc caggaacaac
     1201 tgggccgtcg tattcattat agccagaacg atctggtgga atatagcccg gtgaccgaaa
     1261 aacatctgac cgatggcatg accgtgcgtg aactgtgcag cgcggcgatt accatgagcg
     1321 ataacaccgc ggcgaacctg ctgctgacga ccattggcgg tccgaaagaa ctgaccgcgt
     1381 ttctgcataa catgggcgat catgtgaccc gtctggatcg ttgggaaccg gaactgaacg
     1441 aagcgattcc gaacgatgaa cgtgatacca ccatgccggc agcaatggcg accaccctgc
     1501 gtaaactgct gacgggtgag ctgctgaccc tggcaagccg ccagcaactg attgattgga
     1561 tggaagcgga taaagtggcg ggtccgctgc tgcgtagcgc gctgccggct ggctggttta
     1621 ttgcggataa aagcggtgcg ggcgaacgtg gcagccgtgg cattattgcg gcgctgggcc
     1681 cggatggtaa accgagccgt attgtggtga tttataccac cggcagccag gcgacgatgg
     1741 atgaacgtaa ccgtcagatt gcggaaattg gcgcgagcct gattaaacat tggtaaaccg
     1801 atacaattaa aggctccttt tggagccttt ttttttggac gacccttgtc agatcaaagg
     1861 atcttc
//
