LOCUS       sequence-46483-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Alford SC, Abdelfattah AS, Ding Y, Campbell RE
  TITLE     A fluorogenic red fluorescent protein heterodimer.
  JOURNAL   Chem Biol. 2012 Mar 23;19(3):353-60.
  PUBMED    22444590
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2012-03-23"; volume: "19(3)"; pages: "353-60"
COMMENT     Sequence Label: pDDRFP-B1
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..96
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             100..132
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(136..144,145..159)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(308..326)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     576..595
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     770..793
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa
       61 ttaaccatgg ggggttctca tcatcatcat catcatggta tggctagcat gactggtgga
      121 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccgagctc gagaatggtg
      181 agcaagggcg aggagaccat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
      241 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      301 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
      361 tccccccagt tcatgtacgg ctccgaggcg tacgtgaggc accccgccga catccccgat
      421 tacaagaagc tgcccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaagac
      481 ggcggtctgg tgaccgttac ccaggactcc tccctgcagg acggcacgct gatctgcaag
      541 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
      601 ggctgggagg cctccaccga gatgctgtac cccgaagacg gcgtgctgaa gggccatagc
      661 tatcaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcga gaccatctac
      721 atggccaaga agcccgtgca actgcccggc gattactgtg tggacaccaa gctggacatc
      781 acctcccaca acgaggacta caccatcgtg gaacagtacg ggcgctccga gggccgccac
      841 c
//
