LOCUS       sequence-464840-        2467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Misc_oriT-IncP_PartType0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK120 [Misc_oriT-IncP_PartType0]
FEATURES             Location/Qualifiers
     source          1..2467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            755..864
                     /note="incP origin of transfer"
     CDS             897..1268
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     promoter        1445..1536
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1537..1605,1606..2397)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2401..2440
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgagta tcttccgctg cataaccctg cttcggggtc attatagcga
      661 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
      721 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
      781 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
      841 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg
      901 ctgatgaaac caagccaacc aggaagggca gcccacctat caaggtgtac tgccttccag
      961 acgaacgaag agcgattgag gaaaaggcgg cggcggccgg catgagcctg tcggcctacc
     1021 tgctggccgt cggccagggc tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg
     1081 agctggcccg catcaatggc gacctgggcc gcctgggcgg cctgctgaaa ctctggctca
     1141 ccgacgaccc gcgcacggcg cggttcggtg atgccacgat cctcgccctg ctggcgaaga
     1201 tcgaagagaa gcaggacgag cttggcaagg tcatgatggg cgtggtccgc ccgagggcag
     1261 agccatgact tttttagccg ctaaaacggc cggggggtgc gcgtgattgc caagcacgtc
     1321 cccatgcgct ccatcaagaa gaggcacttc gagctgtaag tacatcaccg acgagcaagg
     1381 caagacgatc ccgatgagac cagagtagcc cgcctaatga gcgggctttt ttttaattcc
     1441 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1501 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gcattcagca ttttcgtgtg
     1561 gcgctgattc cgttttttgc ggcgttttgc ctgccggtgt ttgcgcatcc ggaaaccctg
     1621 gtgaaagtga aagatgcgga agatcaactg ggtgcgcgcg tgggctatat tgaactggat
     1681 ctgaacagcg gcaaaattct ggaatctttt cgtccggaag aacgttttcc gatgatgagc
     1741 acctttaaag tgctgctgtg cggtgcggtt ctgagccgtg tggatgcggg ccaggaacaa
     1801 ctgggccgtc gtattcatta tagccagaac gatctggtgg aatatagccc ggtgaccgaa
     1861 aaacatctga ccgatggcat gaccgtgcgt gaactgtgca gcgcggcgat taccatgagc
     1921 gataacaccg cggcgaacct gctgctgacg accattggcg gtccgaaaga actgaccgcg
     1981 tttctgcata acatgggcga tcatgtgacc cgtctggatc gttgggaacc ggaactgaac
     2041 gaagcgattc cgaacgatga acgtgatacc accatgccgg cagcaatggc gaccaccctg
     2101 cgtaaactgc tgacgggtga gctgctgacc ctggcaagcc gccagcaact gattgattgg
     2161 atggaagcgg ataaagtggc gggtccgctg ctgcgtagcg cgctgccggc tggctggttt
     2221 attgcggata aaagcggtgc gggcgaacgt ggcagccgtg gcattattgc ggcgctgggc
     2281 ccggatggta aaccgagccg tattgtggtg atttatacca ccggcagcca ggcgacgatg
     2341 gatgaacgta accgtcagat tgcggaaatt ggcgcgagcc tgattaaaca ttggtaaacc
     2401 gatacaatta aaggctcctt ttggagcctt tttttttgga cgacccttgt cagatcaaag
     2461 gatcttc
//
