LOCUS       sequence-464916-        2366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the COX20 promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM29
FEATURES             Location/Qualifiers
     source          1..2366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(626..660)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(731..1390)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1305..1324
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1572..2160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2061..2080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaaaaaact gtctttctca cacacactca atttcccacg ccttggacgt ttcgaatgtc
       61 tgacagaact gctgctcaac agggtcccac ctaagcgttc aaacttttcg tgtcaatctc
      121 tatataaact ccagttggtt ggttttcttt ttggtatcca aaaccgatat ttaatgtcca
      181 agaacggaca gagacaatac atgaaagagt aacagaaaca agaatgctga aagccgagcg
      241 aacggattat gcaagaaaag ggaggggata aaagagtact gagatctgca ctgtcactgg
      301 cacaggccac ttctgtatca actcctcttg tcaatataac cccggccgtt acagtagaaa
      361 aaccacctgc tggagccggt ggacacacac tggtacactc cagccaactc tttccagcca
      421 agacttactc tgctttttcc caatttttca tatatcagtc tacttacata cagaaagtaa
      481 agcattaaat atataataaa tagagttgag aagagcaagc gtgaacgtta acgtttggaa
      541 agaagccagt tggaatgggc ttttgaatgt tgtaaataca caagaaaccc tggagattgg
      601 tgtacatata tgtgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa
      661 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
      721 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
      781 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
      841 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
      901 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga
      961 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     1021 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     1081 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     1141 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     1201 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     1261 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     1321 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     1381 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     1441 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg
     1501 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1561 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1621 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1681 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1741 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1801 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1921 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1981 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2041 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2101 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2161 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2221 ttctttcctg cgttatcccc tgattctgtg gataaccgta gtcggtctca aacggaaaat
     2281 tgtcataaac gttcctctgc tggttgcttg ccatgcttac ggcttctctt ctttttaccg
     2341 tgtctatcta tctattcttt ccgtcc
//
