LOCUS       sequence-464960-        8981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible blue light-inducible Cre-N; Cre-N229-pmag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: IK331
FEATURES             Location/Qualifiers
     source          1..8981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(379..483)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(509..964)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(646..667)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     858..877
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1091..1113
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1105..1122
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1106..1122
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1252..1314
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1841..2155
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1849..1867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1885..1903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1920..1938
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1956..1974
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1992..2010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2027..2045
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2063..2081
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2128..2152
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3350..3370
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3380..3604
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3844..3863)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3909..3964
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3963..3982)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        4318..5496
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4549..5487
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5444..5464
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5508..5517
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5929..6050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5966..5985)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6020..6039
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6813..6847
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7338..7354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7338..7354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7351..7373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7362..7378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7386..7392,7393..7410,7411..7416))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7431..7452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7569..7586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7740..8328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7820..7839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8499..8981,1..309,310..378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
       61 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
      121 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
      181 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      241 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      301 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      361 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      421 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      481 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
      541 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
      601 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
      661 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
      721 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
      781 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
      841 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
      901 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
      961 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     1021 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     1081 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt
     1141 cacgtttttg aacccgtgga ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg
     1201 atggagcaga tgaagatgct cgacacgctg cagaacacgc agctagatta accctagaaa
     1261 gataatcata ttgtgacgta cgttaaagat aatcatgtgt aaaattgacg catgtgtttt
     1321 atcggtctgt atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt
     1381 tacacttaca tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta
     1441 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttatg agggacagcc
     1501 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     1561 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     1621 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     1681 gctctttgag cctgcagaca cctgggggga tacggggaaa aggcctccac ggccagtcga
     1741 cattgattat tgactagtat tcgagcgaag gcgcgccgct gggagttcgt agacggaaac
     1801 aaacgcagaa tccaagcgcg aggccctttc gtcttcactc gagtttactc cctatcagtg
     1861 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgatgt cgagtttact
     1921 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     1981 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttatccc tatcagtgat
     2041 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gaggtaggcg
     2101 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgccacgcg
     2161 tgccgccacc atgtccaacc tgctgactgt gcaccaaaac ctgcctgccc tccctgtgga
     2221 tgccacctct gatgaagtca ggaagaacct gatggacatg ttcagggaca ggcaggcctt
     2281 ctctgaacac acctggaaga tgctcctgtc tgtgtgcaga tcctgggctg cctggtgcaa
     2341 gctgaacaac aggaaatggt tccctgctga acctgaggat gtgagggact acctcctgta
     2401 cctgcaagcc agaggcctgg ctgtgaagac catccaacag cacctgggcc agctcaacat
     2461 gctgcacagg agatctggcc tgcctcgccc ttctgactcc aatgctgtgt ccctggtgat
     2521 gaggagaatc agaaaggaga atgtggatgc tggggagaga gccaagcagg ccctggcctt
     2581 tgaacgcact gactttgacc aagtcagatc cctgatggag aactctgaca gatgccagga
     2641 catcaggaac ctggccttcc tgggcattgc ctacaacacc ctgctgcgca ttgccgaaat
     2701 tgccagaatc agagtgaagg acatctcccg caccgatggt gggagaatgc tgatccacat
     2761 tggcaggacc aagaccctgg tgtccacagc tggtgtggag aaggccctgt ccctgggggt
     2821 taccaagctg gtggagagat ggatctctgt gtctggttcc ggagggtctg gctccggatc
     2881 aagtggtggc agcggtaccc atactcttta tgcccccggt ggatatgaca ttatgggata
     2941 tctgaggcag atcaggaacc ggccaaaccc gcaggtggaa ctgggccccg tggatacatc
     3001 ctgcgccttg attctttgtg acctgaaaca gaaagacacc ccgatagttt acgcgagtga
     3061 agccttcctc tacatgacag gttacagcaa cgcagaggtg ctgggccgga attgccggtt
     3121 tctgcaaagc cctgacggca tggtgaagcc caagagcacc cggaagtacg tggatagtaa
     3181 cacaatcaat actatgcgca aggcaatcga caggaatgcc gaggtgcagg ttgaagtagt
     3241 caattttaaa aagaatggac agcgatttgt taatttcctg actatgatac ctgttaggga
     3301 cgaaacaggc gagtatcgat actctatggg attccagtgc gaaacagaac ccaagaaaaa
     3361 gcggaaggtg tgagaattcc tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3421 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3481 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3541 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3601 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     3661 agcgtgtacc gctgggagtt cgtagacgga aacaaacgca gaatccaagc gcggccgccc
     3721 aggatacata gattaccaca actccgagcc cttccaccgc tagcgcactc ctcaggtgca
     3781 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     3841 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     3901 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     3961 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     4021 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     4081 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     4141 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     4201 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     4261 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttcgtgaggc
     4321 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     4381 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     4441 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     4501 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     4561 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     4621 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     4681 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     4741 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     4801 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     4861 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     4921 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     4981 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     5041 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     5101 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     5161 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     5221 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     5281 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     5341 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     5401 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     5461 gttcaaagtt tttttcttcc atttcaggtg tcgtgattaa ttaagccgcc accatggcca
     5521 agcccctgag ccaggaagag agcaccctga tcgagagagc caccgccacc atcaacagca
     5581 tccccatcag cgaggactac agcgtggcct ctgccgccct gtcctccgac ggcagaatct
     5641 tcacaggcgt gaacgtgtac cacttcaccg gcggaccttg cgccgaactg gtggtgctgg
     5701 gaacagctgc cgctgccgca gccggcaatc tgacatgtat cgtggccatc ggcaacgaga
     5761 accggggcat cctgagccct tgcggcagat gcagacaggt gctgctggat ctgcaccccg
     5821 gcatcaaggc catcgtgaag gatagcgacg gccagcctac agccgtgggc atcagagagc
     5881 tgctgcctag cggctatgtg tgggagggct gagtttaaac gttaactaaa cttgtttatt
     5941 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6001 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6061 aattgactca aatgatgtca attagtctat cagaagctca tctggtctcc cttccggggg
     6121 acaagacatc cctgtttaat atttaaacag cagtgttccc aaactgggtt cttatatccc
     6181 ttgctctggt caaccaggtt gcagggtttc ctgtcctcac aggaacgaag tccctaaaga
     6241 aacagtggca gccaggttta gccccggaat tgactggatt ccttttttag ggcccattgg
     6301 tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     6361 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     6421 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     6481 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga
     6541 tatctataac aagaaaatat atatataata agttatcacg taagtagaac atgaaataac
     6601 aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt
     6661 ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc
     6721 ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct
     6781 atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt
     6841 tctagggtta atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
     6901 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga
     6961 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
     7021 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
     7081 actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg
     7141 gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg
     7201 tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg
     7261 gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg
     7321 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7381 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7441 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7501 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7561 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7621 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7681 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7741 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7801 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7861 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7921 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7981 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8041 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8101 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8161 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8221 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8281 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8341 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8401 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8461 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8521 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8581 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8641 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8701 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8761 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8821 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8881 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8941 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca t
//
