LOCUS       sequence-464961-        6598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Cre-N; Cre-N229-FHY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT367
FEATURES             Location/Qualifiers
     source          1..6598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(79..870,871..939))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     702..721
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(940..1044)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1075..1454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1456..1733
                     /label=chicken beta-actin promoter
     intron          1734..2750
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2674..2696
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2758..2777
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4217..4237
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4247..4471
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4711..4730)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4776..4831
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4830..4849)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5192..5208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5192..5208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5205..5227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5216..5232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5240..5246,5247..5264,5265..5270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5285..5306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      5391..5525
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5545..5679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5582..5601)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5636..5655
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5747..5764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5918..6506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5998..6017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
       61 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
      121 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
      181 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
      241 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
      301 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
      361 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
      421 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
      481 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
      541 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
      601 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
      661 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
      721 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
      781 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
      841 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
      901 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
      961 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     1021 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt
     1081 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     1141 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     1201 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     1261 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     1321 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     1381 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     1441 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
     1501 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
     1561 cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
     1621 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     1681 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     1741 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     1801 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     1861 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     1921 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     1981 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     2041 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     2101 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     2161 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     2221 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     2281 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     2341 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     2401 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     2461 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     2521 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     2581 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     2641 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     2701 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     2761 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgattaat tcgagcgaag
     2821 gcgcgcccat tactcgcatc cattctcagg ctgtctcgtc tcgtctcgca cgcgtgccgc
     2881 caccatgtcc aacctgctga ctgtgcacca aaacctgcct gccctccctg tggatgccac
     2941 ctctgatgaa gtcaggaaga acctgatgga catgttcagg gacaggcagg ccttctctga
     3001 acacacctgg aagatgctcc tgtctgtgtg cagatcctgg gctgcctggt gcaagctgaa
     3061 caacaggaaa tggttccctg ctgaacctga ggatgtgagg gactacctcc tgtacctgca
     3121 agccagaggc ctggctgtga agaccatcca acagcacctg ggccagctca acatgctgca
     3181 caggagatct ggcctgcctc gcccttctga ctccaatgct gtgtccctgg tgatgaggag
     3241 aatcagaaag gagaatgtgg atgctgggga gagagccaag caggccctgg cctttgaacg
     3301 cactgacttt gaccaagtca gatccctgat ggagaactct gacagatgcc aggacatcag
     3361 gaacctggcc ttcctgggca ttgcctacaa caccctgctg cgcattgccg aaattgccag
     3421 aatcagagtg aaggacatct cccgcaccga tggtgggaga atgctgatcc acattggcag
     3481 gaccaagacc ctggtgtcca cagctggtgt ggagaaggcc ctgtccctgg gggttaccaa
     3541 gctggtggag agatggatct ctgtgtctgg ttccggaggg tctggctccg gatcaagtgg
     3601 tggcagcggt acccccgagg tggaggtcga caacaacaac gagaagccca gcgagatcaa
     3661 cagcttccac cacatgatta tcagcagcag caagaacgtg ctgaagatgg aggaggtgga
     3721 ggtgtccaag aagaggaagt tccagaccga ccagagcgac gagctgagct tattaccttt
     3781 atccaaacac acatgctttg ccaacgtggc ttgtagcgag aacaccaacg gcaacagcga
     3841 gatcgatacc gagtacagca tgtccagcta cgtgaactcc accacctcca tggagtgcaa
     3901 caacgacatc gagatgaagg aagaatccag cggcagctgc ggagaggaca agatgatcag
     3961 cttcgagtcc catttagact atatctacgg cacccagaat ctggaggact tctccgagaa
     4021 ggtgatcgag aacattctgt acctcgacga gcaagaagag gaggaggagg acgccaaggg
     4081 ctgtagcagc aacgctgcca agttcgtgct gtcctctggt cgttggaccg tgaaccaaga
     4141 tgatagcact ttacacgaaa ccaagaagcc caccatcgac caagaattcg agcagtactt
     4201 tagcacttta atgctgccca agaaaaagcg gaaggtgtga gaattcctgt gccttctagt
     4261 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4321 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4381 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4441 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     4501 tctagggggt atccccacgc gccctgtagc gtgtaccgct gggagttcgt agacggaaac
     4561 aaacgcagaa tccaagcgcg gccgcccagg atacatagat taccacaact ccgagccctt
     4621 ccaccgctag cgcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     4681 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     4741 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     4801 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     4861 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     4921 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     4981 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     5041 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     5101 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     5161 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5221 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5281 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5341 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
     5401 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5461 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5521 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa
     5581 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5641 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc
     5701 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5761 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5821 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5881 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5941 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6001 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6061 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6121 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6181 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6241 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6301 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6361 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6421 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6481 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6541 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggat
//
