LOCUS       sequence-464977-        1180 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector that can express hAqp1 with fkbp12 degron in 
            mammalian cells. EF1a promoter. EGFP marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Yun J, Huang Y, Miller ADC, Chang BL, Baldini L, Dhanabalan KM, Li 
            E, Li H, Mukherjee A
  TITLE     Destabilized reporters for background-subtracted, chemically-gated, 
            and multiplexed deep-tissue imaging.
  JOURNAL   Chem Sci. 2024 Jun 4;15(28):11108-11121. doi: 10.1039/d4sc00377b. 
            eCollection 2024 Jul 17.
  PUBMED    39027298
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/d4sc00377b"; journalName: "Chem Sci"; date: "2024-06-4- 4";
            volume: "15"; issue: "28"; pages: "11108-11121"
COMMENT     Sequence Label: pL(EF1a-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)
FEATURES             Location/Qualifiers
     source          1..1180
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..52
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             857..1177
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
ORIGIN
        1 gagctcggat ccagtaccct tcaccatgga ctacaaggac gacgacgaca aggccagcga
       61 gttcaagaag aagctcttct ggagggcagt ggtggccgag ttcctggcca cgaccctctt
      121 tgtcttcatc agcatcggtt ctgccctggg cttcaaatac ccggtgggga acaaccagac
      181 ggcggtccag gacaacgtga aggtgtcgct ggccttcggg ctgagcatcg ccacgctggc
      241 gcagagtgtg ggccacatca gcggcgccca cctcaacccg gctgtcacac tggggctgct
      301 gctcagctgc cagatcagca tcttccgtgc cctcatgtac atcatcgccc agtgcgtggg
      361 ggccatcgtc gccaccgcca tcctctcagg catcacctcc tccctgactg ggaactcgct
      421 tggccgcaat gacctggctg atggtgtgaa ctcgggccag ggcctgggca tcgagatcat
      481 cgggaccctc cagctggtgc tatgcgtgct ggctactacc gaccggaggc gccgtgacct
      541 tggtggctca gccccccttg ccatcggcct ctctgtagcc cttggacacc tcctggctat
      601 tgactacact ggctgtggga ttaaccctgc tcggtccttt ggctccgcgg tgatcacaca
      661 caacttcagc aaccactgga ttttctgggt ggggccattc atcgggggag ccctggctgt
      721 actcatctac gacttcatcc tggccccacg cagcagtgac ctcacagacc gcgtgaaggt
      781 gtggaccagc ggccaggtgg aggagtatga cctggatgcc gacgacatca actccagggt
      841 ggagatgaag cccaaaggag tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt
      901 ccccaagcgc ggccagacct gtgtggtgca ctacaccggg atgcttgaag atggaaagaa
      961 agtcgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt
     1021 gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac
     1081 tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc
     1141 cactctcgtc ttcgatgtgg agcttctaaa accggaatag
//
