LOCUS       sequence-464990-        8639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible blue light-inducible Cre-C; Cre-230C-nmag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 8639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: IK332
FEATURES             Location/Qualifiers
     source          1..8639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     436..455
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(674..778)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(804..1259)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(941..962)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1153..1172
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1386..1408
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1400..1417
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1401..1417
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1547..1609
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2136..2450
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2144..2162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2180..2198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2215..2233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2251..2269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2287..2305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2322..2340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2358..2376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2423..2447
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3303..3323
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3333..3557
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3797..3816)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3862..3917
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3916..3935)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        4271..5449
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4502..5440
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5397..5417
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5461..5470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5882..6003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5919..5938)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5973..5992
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6766..6800
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7291..7307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7291..7307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7304..7326)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7315..7331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7339..7345,7346..7363,7364..7369))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7384..7405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7522..7539)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7693..8281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7773..7792)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8452..8639,1..604,605..673))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
       61 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
      121 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
      181 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
      241 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
      301 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
      361 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
      421 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
      481 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
      541 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
      601 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
      661 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      721 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
      781 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
      841 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
      901 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
      961 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1021 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1081 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1141 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1201 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1261 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     1321 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     1381 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcctcgt tcattcacgt
     1441 ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat gtgttttaca gcgtgatgga
     1501 gcagatgaag atgctcgaca cgctgcagaa cacgcagcta gattaaccct agaaagataa
     1561 tcatattgtg acgtacgtta aagataatca tgtgtaaaat tgacgcatgt gttttatcgg
     1621 tctgtatatc gaggtttatt tattaatttg aatagatatt aagttttatt atatttacac
     1681 ttacatacta ataataaatt caacaaacaa tttatttatg tttatttatt tattaaaaaa
     1741 aacaaaaact caaaatttct tctataaagt aacaaaactt ttatgaggga cagccccccc
     1801 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc
     1861 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac
     1921 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct
     1981 ttgagcctgc agacacctgg ggggatacgg ggaaaaggcc tccacggcca gtcgacattg
     2041 attattgact agtattcgag cgaaggcgcg ccgctgggag ttcgtagacg gaaacaaacg
     2101 cagaatccaa gcgcgaggcc ctttcgtctt cactcgagtt tactccctat cagtgataga
     2161 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
     2221 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     2281 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
     2341 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
     2401 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc acgcgtgccg
     2461 ccaccatgca tactctttat gcccccggtg gatatgacat tatgggatat ctggaccaga
     2521 tcggcaaccg gccaaacccg caggtggaac tgggccccgt ggatacatcc tgcgccttga
     2581 ttctttgtga cctgaaacag aaagacaccc cgatagttta cgcgagtgaa gccttcctct
     2641 acatgacagg ttacagcaac gcagaggtgc tgggccggaa ttgccggttt ctgcaaagcc
     2701 ctgacggcat ggtgaagccc aagagcaccc ggaagtacgt ggatagtaac acaatcaata
     2761 ctatgcgcaa ggcaatcgac aggaatgccg aggtgcaggt tgaagtagtc aattttaaaa
     2821 agaatggaca gcgatttgtt aatttcctga ctatgatacc tgttagggac gaaacaggcg
     2881 agtatcgata ctctatggga ttccagtgcg aaacagaatc cggagggtct ggctccggat
     2941 caagtggtgg cagcggtacc gtggctgatg accccaacaa ctacctgttc tgccgggtca
     3001 gaaagaatgg tgtggctgcc ccttctgcca cctcccaact gtccacccgg gccctggaag
     3061 ggatctttga ggccacccac cgcctgatct atggtgccaa ggatgactct gggcagagat
     3121 acctggcctg gtctggccac tctgccagag tgggtgctgc cagggacatg gccagggctg
     3181 gtgtgtccat ccctgaaatc atgcaggctg gtggctggac caatgtgaac attgtgatga
     3241 actacatcag aaacctggac tctgagactg gggccatggt gaggctgctc gaggatgggg
     3301 accccaagaa aaagcggaag gtgtgagaat tcctgtgcct tctagttgcc agccatctgt
     3361 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3421 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3481 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     3541 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     3601 ccacgcgccc tgtagcgtgt accgctggga gttcgtagac ggaaacaaac gcagaatcca
     3661 agcgcggccg cccaggatac atagattacc acaactccga gcccttccac cgctagcgca
     3721 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     3781 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     3841 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     3901 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     3961 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     4021 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     4081 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     4141 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     4201 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     4261 agcttcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     4321 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     4381 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     4441 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     4501 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     4561 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     4621 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     4681 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     4741 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     4801 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     4861 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     4921 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     4981 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     5041 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     5101 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     5161 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     5221 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     5281 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     5341 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     5401 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgat taattaagcc
     5461 gccaccatgg ccaagcccct gagccaggaa gagagcaccc tgatcgagag agccaccgcc
     5521 accatcaaca gcatccccat cagcgaggac tacagcgtgg cctctgccgc cctgtcctcc
     5581 gacggcagaa tcttcacagg cgtgaacgtg taccacttca ccggcggacc ttgcgccgaa
     5641 ctggtggtgc tgggaacagc tgccgctgcc gcagccggca atctgacatg tatcgtggcc
     5701 atcggcaacg agaaccgggg catcctgagc ccttgcggca gatgcagaca ggtgctgctg
     5761 gatctgcacc ccggcatcaa ggccatcgtg aaggatagcg acggccagcc tacagccgtg
     5821 ggcatcagag agctgctgcc tagcggctat gtgtgggagg gctgagttta aacgttaact
     5881 aaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5941 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6001 ttatcatgtc tggaattgac tcaaatgatg tcaattagtc tatcagaagc tcatctggtc
     6061 tcccttccgg gggacaagac atccctgttt aatatttaaa cagcagtgtt cccaaactgg
     6121 gttcttatat cccttgctct ggtcaaccag gttgcagggt ttcctgtcct cacaggaacg
     6181 aagtccctaa agaaacagtg gcagccaggt ttagccccgg aattgactgg attccttttt
     6241 tagggcccat tggtatggct ttttccccgt atccccccag gtgtctgcag gctcaaagag
     6301 cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc
     6361 ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg
     6421 gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg
     6481 gggctgtccc tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag
     6541 aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata
     6601 atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca
     6661 ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg
     6721 acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta
     6781 cgcagactat ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt
     6841 tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg
     6901 ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca
     6961 ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg
     7021 cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc
     7081 ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca
     7141 gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg
     7201 tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt
     7261 tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7321 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7381 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7441 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7501 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7561 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7621 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7681 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7741 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7801 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7861 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7921 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7981 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8041 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8101 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8161 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8221 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8281 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8341 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8401 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8461 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8521 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8581 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccag
//
