LOCUS       sequence-465006-        7434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Cre-C; Cre-252C-PhyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 7434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT363
FEATURES             Location/Qualifiers
     source          1..7434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(107..126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      920..1508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1409..1428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1662..1679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1747..1881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1771..1790)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1825..1844
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1901..2035
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2120..2141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2156..2161,2162..2179,2180..2186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2194..2210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2199..2221
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2218..2234
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2218..2234
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2577..2596
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2595..2650
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2696..2715
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2955..3179
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3189..3209)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5375..5384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5486..5505)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5513..6529
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5567..5589)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6530..6806)
                     /label=chicken beta-actin promoter
     enhancer        6808..7187
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7218..7322
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7323..7391,7392..7434,1..749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
       61 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      121 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      421 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      601 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
      781 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
      841 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
      901 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      961 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1021 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1081 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1141 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1201 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1261 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1501 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1561 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1621 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1681 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1741 gcagcggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1801 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1861 ataagctgca ataaacaagt tagctttttg caaaagccta ggcctccaaa aaagcctcct
     1921 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     1981 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggactatg
     2041 gttgctgact aattgagatg cggatccgct ggcacgacag gtttcccgac tggaaagcgg
     2101 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     2161 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     2221 gaaacagcta tgaccatgat tacgccaagc ttgggctgca ggtcgaggga tctccataag
     2281 agaagaggga cagctatgac tgggagtagt caggagagga ggaaaaatct ggctagtaaa
     2341 acatgtaagg aaaattttag ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa
     2401 aaaatctaac ctcaagtcaa ggcttttcta tggaataagg aatggacagc agggggctgt
     2461 ttcatatact gatgacctct ttatagccaa cctttgttca tggcagccag catatgggca
     2521 tatgttgcca aactctaaac caaatactca ttctgatgtt ttaaatgatt tgccctccca
     2581 tatgtccttc cgagtgagag acacaaaaaa ttccaacaca ctattgcaat gaaaataaat
     2641 ttcctttatt agccagaagt cagatgctca aggggcttca tgatgtcccc ataatttttg
     2701 gcagagggaa aaagatctca gtggtatttg tgagccaggg cattggccac accagccacc
     2761 accttctgat aggcagcctg cacctgagga gtgcgctagc ggtggaaggg ctcggagttg
     2821 tggtaatcta tgtatcctgg gcggccgcgc ttggattctg cgtttgtttc cgtctacgaa
     2881 ctcccagcgg tacacgctac agggcgcgtg gggatacccc ctagagcccc agctggttct
     2941 ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca
     3001 atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag
     3061 acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag
     3121 ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagg
     3181 aattctcaca ccttccgctt tttcttgggg tccccatcct cgagcagcct caccatggcc
     3241 ccagtctcag agtccaggtt tctgatgtag ttcatcacaa tgttcacatt ggtccagcca
     3301 ccagcctgca tgatttcagg gatggacaca ccagccctgg ccatgtccct ggcagcaccc
     3361 actctggcag agtggccaga ccaggccagg tatctctgcc cagagtcatc cttggcacca
     3421 tagatcaggc ggtgggtggc ctcaaagatc ccttccaggg cccgggtgga cagttgggag
     3481 gtggcggtac cgctgccacc acttgatccg gagccagacc ctccggattg gattccatca
     3541 atcttgagat cgttgagctt gctatagatc acctttgtgt tcacgtcggt ggtctcgctg
     3601 tccttaaagg cgttacgtaa gatgagctgt aaagagtgga tggcatccat ttcgtagtcc
     3661 ttccaaggta agcttctggt cttcaccacc tccagaaaag ccttgaagga ggatctgggg
     3721 tgcatccttc tggcatcgtc cctatcatcg gggtcgtgtt tggcgccgcc ccatctcact
     3781 tcgccggcgg tatgggaacg aaaccagaaa atcatatctt tgctgctgat acgaacggca
     3841 gccattccgc agacgctgtc gcctaaagat aaagccctag gaaagccagc gtcgtgtaaa
     3901 gaatcggtgc ttaaaccggt ggaatccatg tggtactcac acagccaaga agcgatttct
     3961 tgtaaatgga actcgctagg ggtggtgccc agcttccaga tcttgtcctt gtataataag
     4021 gcggcgccgt cgcacttgac taaatccata atattggggg actggctcac gatgccgagg
     4081 ggagcgtccc tcatgagcat gtcgcagagg agtgtctggg tacgtaaaat gttcttctcc
     4141 accatctggt tgtccagctc gacctccttg ttgacgtgga tggcaaacac ttgggctaaa
     4201 aattcacaag cataacgtaa gggaaaaggg acaaatctgg gggtggtgtt gtggcagacc
     4261 actaaacccc acagcctctt tctcttctga ggctgtgtgg tggcatcggg agcatcgccc
     4321 tctccatcct cctcattcac caccacggcc atcactaaag aagcgatgct gtccatattg
     4381 gccatgtatt gcagatgaca agagtgggga gctcttaaag tgctgccgca gagggttaaa
     4441 tcgaagctca gcttctcatc ttgtaaaacc ctagcgtgct tggcgttgca gtccacgatc
     4501 attctcacct tgttcttcat aaataaaaat ctggcagctt gggggatgtc tgtggcggga
     4561 tagtgtaaac ctaaataggg ttctaaaccg ggcttggtca cttcgctcac cacctcgccg
     4621 tggtcgtcct cgtggaactt gtaggccatg acacgatcgt aaccagtcag ctcgaagact
     4681 tcttgcacca tggtgtcgca cagcctctcc atgcttccgc tgggtaaaga ctggagtctg
     4741 gtgatggcct tggcggccag cttatagctc tgtaaagctc cggcggctgt catgggcact
     4801 tcatagggct taacgggttc gaagtcgatg atgatggatc cggtcaccct atggataatg
     4861 gcgtagaagg gcttggcgga tgtacgacaa tggacgagga tagggttcag taaagacacg
     4921 tcgccgaagc cgagagcctt ctgtaaagcg gaagcgctgg gagcggtaaa taaagatctg
     4981 atatcggtgc cgatgcccag aacggggtgc tctcccacgc taggcacggc gtgggaagcc
     5041 attgtgagca gttcggaggc gttctcggag taggcgatga ccttgaaggt cttttcgtct
     5101 aaagccagta aacatccaaa gggctggatc agcttgccct tctggatatg atggaggtag
     5161 gttgtggtga ccttgtcgct cctaggaggc tggttctcga ccacgggacc agtcaccctc
     5221 acgctggtgg agtaatcaaa ggaggagccg ctctcctcga aatcggcgtg cagtttggcg
     5281 tccacggtgg tctgagcgat gatacgagcg gagtgacgag atctacgaga tccctcgctg
     5341 gactgtgtgg gacgagatcc gctcatcttc ttctccatgg tggcggcacg cgtgcgagac
     5401 gagacgagac agcctgagaa tggatgcgag taatgggcgc gccttcgctc gaattaatca
     5461 attctttgcc aaaatgatga gacagcacaa taaccagcac gttgcccagg agctgtagga
     5521 aaaagaagaa ggcatgaaca tggttagcag aggctctaga gccgccggtc acacgccaga
     5581 agccgaaccc cgccctgccc cgtccccccc gaaggcagcc gtccccccgc ggacagcccc
     5641 gaggctggag agggagaagg ggacggcggc gcggcgacgc acgaaggccc tccccgccca
     5701 tttccttcct gccggcgccg caccgcttcg ccccgcgccc gctagagggg gtgcggcggc
     5761 gcctcccaga tttcggctcc gcacagattt gggacaaagg aagtccctgc gccctctcgc
     5821 acgattacca taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg ccggcgctcc
     5881 gggggccgcc gcgcccctcc cccgagccct ccccggcccg aggcggcccc gccccgcccg
     5941 gcacccccac ctgccgccac cccccgcccg gcacggcgag ccccgcgcca cgccccgtac
     6001 ggagccccgc acccgaagcc gggccgtgct cagcaactcg gggagggggg tgcagggggg
     6061 gttgcagccc gaccgacgcg cccacacccc ctgctcaccc ccccacgcac acaccccgca
     6121 cgcagccttt gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc ccggccgcgc
     6181 tcccctcgcg cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag cgctcacagc
     6241 cgccgggcag cgcggagccg cacgcggcgc tccccacgca cacacacacg cacgcacccc
     6301 ccgagccgct ccccccgcac aaagggccct cccggagccc ctcaaggctt tcacgcagcc
     6361 acagaaaaga aacaagccgt cattaaacca agcgctaatt acagcccgga ggagaagggc
     6421 cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc gggcggcgcg
     6481 aggcggcggc ggagcggggc acggggcgaa ggcagcgcgc agcgactccc gcccgccgcg
     6541 cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga aactttcgga
     6601 gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc cgcccctcgc
     6661 cccgccccgc cccgcctggc gcgcgccccc cccccccccc gcccccatcg ctgcacaaaa
     6721 taattaaaaa ataaataaat acaaaattgg gggtggggag gggggggaga tggggagagt
     6781 gaagcagaac gtggggctca cctcgaccat ggtaatagcg atgactaata cgtagatgta
     6841 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     6901 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     6961 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     7021 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     7081 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     7141 tgaccccgta attgattact attaataact agtcaataat caatgtcgac ccaggtggca
     7201 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7261 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7321 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7381 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagt
//
